##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062428_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506065
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258063894984613	32.0	32.0	32.0	32.0	32.0
2	31.04996397897833	32.0	32.0	32.0	32.0	32.0
3	31.028579111791323	32.0	32.0	32.0	32.0	32.0
4	31.04869112554903	32.0	32.0	32.0	32.0	32.0
5	30.95363015540498	32.0	32.0	32.0	32.0	32.0
6	34.541006530262635	36.0	36.0	36.0	32.0	36.0
7	34.47286737292215	36.0	36.0	36.0	32.0	36.0
8	34.47268278593553	36.0	36.0	36.0	32.0	36.0
9	34.5670884058789	36.0	36.0	36.0	32.0	36.0
10	34.36316028856656	36.0	36.0	36.0	32.0	36.0
11	34.547609166935025	36.0	36.0	36.0	32.0	36.0
12	34.40521690630882	36.0	36.0	36.0	32.0	36.0
13	34.48323877123497	36.0	36.0	36.0	32.0	36.0
14	34.420556217693125	36.0	36.0	36.0	32.0	36.0
15	34.379897945971784	36.0	36.0	36.0	32.0	36.0
16	34.37426737889799	36.0	36.0	36.0	32.0	36.0
17	34.31959775972484	36.0	36.0	36.0	32.0	36.0
18	34.31996826166201	36.0	36.0	36.0	32.0	36.0
19	34.30640111814563	36.0	36.0	36.0	32.0	36.0
20	34.28570944813139	36.0	36.0	36.0	32.0	36.0
21	34.26544538250341	36.0	36.0	36.0	32.0	36.0
22	34.22418355117475	36.0	36.0	36.0	32.0	36.0
23	34.19506528602683	36.0	36.0	36.0	32.0	36.0
24	34.170978012237185	36.0	36.0	36.0	32.0	36.0
25	33.68556602802668	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	60.0
5	121.0
6	264.0
7	58.0
8	241.0
9	287.0
10	167.0
11	56.0
12	126.0
13	110.0
14	236.0
15	338.0
16	524.0
17	756.0
18	1008.0
19	1414.0
20	1845.0
21	2463.0
22	3438.0
23	4788.0
24	6514.0
25	9108.0
26	12877.0
27	17222.0
28	24216.0
29	33551.0
30	45189.0
31	64923.0
32	98094.0
33	149908.0
34	359987.0
35	666167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.135320167310006	17.63607882614727	11.131268231883325	26.0973327746594
2	17.03675766553831	19.919775621403616	36.984886051104034	26.058580661954046
3	19.50183002300145	23.64136064467806	28.071663063283204	28.785146269037288
4	13.048311584819206	15.343825746256268	35.45545705083508	36.15240561808945
5	14.909765996027977	36.15065790784641	33.407570755813566	15.53200534031205
6	34.15992070925887	34.927119745228005	16.927536925065734	13.985422620447391
7	30.078504200954807	30.12368647488563	20.906236814915466	18.891572509244096
8	28.037838444939332	32.84034895691072	19.706947437817046	19.4148651603329
9	27.40224592447654	14.364292778374468	18.81918819188192	39.41427310526708
10	16.167503849089115	26.60425247897294	31.494722958653202	25.73352071328474
11	37.44075955409038	21.096013896616988	22.29288862031669	19.17033792897595
12	24.857165843205227	23.582113766001314	28.696218194342215	22.864502196451237
13	29.669911793879024	19.616621149790852	25.134489736764426	25.5789773195657
14	23.318015976853772	19.85545574454053	25.066149174032827	31.760379104572866
15	25.070562036760062	27.498321534217435	22.209259813208362	25.221856615814136
16	25.55396902001295	25.66498219136012	24.28855181656104	24.492496972065894
17	23.9893975243605	25.751291237666845	25.478185080612185	24.781126157360468
18	24.691087153813164	24.679584991340136	26.782486129256384	23.846841725590316
19	25.442004818969504	24.901134000329776	25.469264442281403	24.187596738419316
20	25.55453691472381	24.463645713320787	25.365597138114175	24.61622023384123
21	26.29864323188322	24.217848810808405	24.571684255386483	24.911823701921897
22	25.741434550670512	24.765091030966087	24.938255349540555	24.555219068822844
23	24.324631629341987	24.5092159157402	25.60594978456301	25.560202670354805
24	24.690877007047145	24.988281351291512	25.471521855196734	24.849319786464612
25	24.641553766768766	24.723332364844243	25.55095837203411	25.08415549635287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	139.0
2	448.5
3	758.0
4	758.0
5	758.0
6	1836.0
7	2914.0
8	2914.0
9	2914.0
10	3292.0
11	3670.0
12	3670.0
13	3670.0
14	4218.5
15	4767.0
16	4767.0
17	4767.0
18	7820.5
19	10874.0
20	10874.0
21	10874.0
22	17169.5
23	23465.0
24	23465.0
25	23465.0
26	35081.5
27	46698.0
28	46698.0
29	46698.0
30	58609.0
31	70520.0
32	70520.0
33	70520.0
34	84010.5
35	97501.0
36	97501.0
37	97501.0
38	109734.0
39	121967.0
40	121967.0
41	121967.0
42	138014.0
43	154061.0
44	154061.0
45	154061.0
46	172927.0
47	191793.0
48	191793.0
49	191793.0
50	193375.0
51	194957.0
52	194957.0
53	194957.0
54	181191.5
55	167426.0
56	167426.0
57	167426.0
58	154127.0
59	140828.0
60	140828.0
61	140828.0
62	125536.0
63	110244.0
64	110244.0
65	110244.0
66	92093.0
67	73942.0
68	73942.0
69	73942.0
70	57216.5
71	40491.0
72	40491.0
73	40491.0
74	32067.0
75	23643.0
76	23643.0
77	23643.0
78	19124.0
79	14605.0
80	14605.0
81	14605.0
82	10456.5
83	6308.0
84	6308.0
85	6308.0
86	4547.0
87	2786.0
88	2786.0
89	2786.0
90	1892.0
91	998.0
92	998.0
93	998.0
94	592.5
95	187.0
96	187.0
97	187.0
98	355.0
99	523.0
100	523.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0078349872017476
2	0.002589529668374207
3	0.004847068353623515
4	0.014275612274370627
5	0.03552303519436412
6	0.04774030337336038
7	0.06971810645622864
8	0.08977036183697251
9	0.09780454362859503
10	0.12037993048108814
11	0.12044632867771311
12	0.13598350668795833
13	0.12356704391908716
14	0.12542619342458658
15	0.11387290721184012
16	0.11546646393083963
17	0.1253597952279616
18	0.13279639324995934
19	0.13339397701958414
20	0.12496140604821174
21	0.13120283653095982
22	0.15211826846782842
23	0.14242413176058139
24	0.1362490994744583
25	0.13306198603645925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1506065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.57372673288681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8451198161852	44.37290869619282
2	12.611646273365148	14.017523672956504
3	3.4518490982921217	5.754963555349443
4	1.355872876244889	3.014036348358669
5	0.6649051000284125	1.8475627166140884
6	0.42404747796901554	1.4139539197451947
7	0.28643047561956164	1.114260628603662
8	0.20321110937905987	0.9034558929374915
9	0.15170069899583974	0.7587515872064443
>10	0.8229387543881229	8.653732569842356
>50	0.0862937502815276	3.3355110209103174
>100	0.08579902751143291	9.9212817638083
>500	0.0073096240720757445	2.7452686954304246
>1k	0.0028759176677019323	2.146788932044212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3290	0.2184500668961831	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2064	0.13704587783395802	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1663	0.11042020098734119	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1584	0.1051747434539678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.639819662497967E-5	0.0
8	0.0	0.0	0.0	6.639819662497967E-5	0.0
9	0.0	0.0	0.0	1.3279639324995934E-4	0.0
10	0.0	0.0	0.0	1.3279639324995934E-4	0.0
11	0.0	0.0	0.0	1.3279639324995934E-4	0.0
12	0.0	6.639819662497967E-5	0.0	1.3279639324995934E-4	1.3279639324995934E-4
13	0.0	6.639819662497967E-5	0.0	1.3279639324995934E-4	2.655927864999187E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGT	45	6.752911E-4	14.780441	16
CGAAATC	45	6.7597267E-4	14.778476	13
ATTCGCA	70	1.0907126E-4	12.214457	14
GAGTGTT	245	0.0	12.022176	7
TTAGACT	105	2.7236456E-7	11.760506	4
CGAACGA	130	2.6066118E-9	11.694416	16
GCGTTAT	155	2.5465852E-11	11.63565	1
CGACCAT	205	0.0	11.586684	10
CCGACCA	205	0.0	11.586684	9
CAAGACG	320	0.0	11.576748	4
CTAACAC	140	6.8394E-10	11.53089	3
GTATAAG	165	7.2759576E-12	11.505746	1
AACCGCG	175	1.8189894E-12	11.401677	7
AGAGTGT	250	0.0	11.401676	6
GACCATA	225	0.0	11.400918	11
GTGCTAA	100	1.9483086E-6	11.390689	1
ATCGCCA	260	0.0	11.328966	16
CGCAAGA	320	0.0	11.274658	2
CGCATCG	255	0.0	11.176999	13
CGCGTAA	145	1.2150849E-9	11.139198	10
>>END_MODULE