FastQCFastQC Report
Thu 2 Feb 2017
SRR4062428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506065
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG450.001398416124.4443154
CGCAATA4350.019.72403336
TAGGACG8200.018.2438054
TTAGGAC5600.017.2856223
TACACGG1154.2745105E-617.21735
TAGCGGC5000.017.1599130
TCTAGCG5000.017.1599128
AGGACGT8150.017.0060445
GTATAGG2201.8189894E-1217.0027311
CAATACG4950.016.88880238
GACGTGA3650.016.8766257
ATACGAA5100.016.8234440
GTCCTAA4600.016.7418211
GGACGTG8050.016.3974296
GTATCAA29700.016.3730011
AATACGA5250.016.3427739
TGTAGGA9250.016.1739622
CGAATGC5450.016.14670643
GCAATAC5450.016.14670637
GTATATC2600.016.0768383
Produced by FastQC (version 0.11.5)