##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506065
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262980017462727	32.0	32.0	32.0	32.0	32.0
2	31.333659569806084	32.0	32.0	32.0	32.0	32.0
3	31.43751962896688	32.0	32.0	32.0	32.0	32.0
4	31.563739280841133	32.0	32.0	32.0	32.0	32.0
5	31.47699269287846	32.0	32.0	32.0	32.0	32.0
6	35.06842599754991	36.0	36.0	36.0	36.0	36.0
7	35.08795769106911	36.0	36.0	36.0	36.0	36.0
8	35.01635121990087	36.0	36.0	36.0	36.0	36.0
9	35.16436408787138	36.0	36.0	36.0	36.0	36.0
10	35.01997988134642	36.0	36.0	36.0	36.0	36.0
11	35.16994552027967	36.0	36.0	36.0	36.0	36.0
12	35.07476569736366	36.0	36.0	36.0	36.0	36.0
13	35.11900814373882	36.0	36.0	36.0	36.0	36.0
14	35.0780484242048	36.0	36.0	36.0	36.0	36.0
15	35.03051063533115	36.0	36.0	36.0	36.0	36.0
16	35.05319956309987	36.0	36.0	36.0	36.0	36.0
17	35.02128194998224	36.0	36.0	36.0	36.0	36.0
18	35.031379123743	36.0	36.0	36.0	36.0	36.0
19	35.02158074186705	36.0	36.0	36.0	36.0	36.0
20	35.007720118321586	36.0	36.0	36.0	36.0	36.0
21	35.00631181257117	36.0	36.0	36.0	36.0	36.0
22	34.98836769993327	36.0	36.0	36.0	36.0	36.0
23	34.94669419978553	36.0	36.0	36.0	32.0	36.0
24	34.934364718654244	36.0	36.0	36.0	32.0	36.0
25	34.90139867801191	36.0	36.0	36.0	32.0	36.0
26	34.859363971674526	36.0	36.0	36.0	32.0	36.0
27	34.841476961485725	36.0	36.0	36.0	32.0	36.0
28	34.829806150464954	36.0	36.0	36.0	32.0	36.0
29	34.805473867329766	36.0	36.0	36.0	32.0	36.0
30	34.784643425084575	36.0	36.0	36.0	32.0	36.0
31	34.7858253129845	36.0	36.0	36.0	32.0	36.0
32	34.74269238047495	36.0	36.0	36.0	32.0	36.0
33	34.725773455992936	36.0	36.0	36.0	32.0	36.0
34	34.71488415174644	36.0	36.0	36.0	32.0	36.0
35	34.696836457921805	36.0	36.0	36.0	32.0	36.0
36	34.66886621759353	36.0	36.0	36.0	32.0	36.0
37	34.66591946562731	36.0	36.0	36.0	32.0	36.0
38	34.62981212630265	36.0	36.0	36.0	32.0	36.0
39	34.61718385328655	36.0	36.0	36.0	32.0	36.0
40	34.618581535325504	36.0	36.0	36.0	32.0	36.0
41	34.5871984276907	36.0	36.0	36.0	32.0	36.0
42	34.563101194171566	36.0	36.0	36.0	32.0	36.0
43	34.54626061956157	36.0	36.0	36.0	32.0	36.0
44	34.515134472947715	36.0	36.0	36.0	32.0	36.0
45	34.48313917394004	36.0	36.0	36.0	32.0	36.0
46	34.482324468067446	36.0	36.0	36.0	32.0	36.0
47	34.467688313585406	36.0	36.0	36.0	32.0	36.0
48	34.410698077440216	36.0	36.0	36.0	32.0	36.0
49	34.40565115051475	36.0	36.0	36.0	32.0	36.0
50	33.846896382294254	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	23.0
22	111.0
23	370.0
24	912.0
25	2163.0
26	4504.0
27	8536.0
28	14417.0
29	23038.0
30	34320.0
31	50510.0
32	76348.0
33	128823.0
34	283212.0
35	878773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4162878649529	18.27930497494762	11.631077567744356	26.67332959235512
2	16.304584558423173	20.49392700722172	36.658406440320526	26.54308199403458
3	18.833824352561766	23.947728197783473	28.435823222453955	28.782624227200802
4	12.790318586033367	15.900949563131906	35.6769745714151	35.631757279419624
5	14.697639212118998	36.28814161407376	33.306198603645925	15.708020570161315
6	34.43282691159764	35.126027766545356	16.64365956932573	13.79748575253128
7	30.182356370454865	30.173458994384028	20.85949980810883	18.784684827052274
8	28.008020902152296	33.09146683576074	19.436876894423545	19.463635367663414
9	27.50505456271808	14.143347066693668	18.612277690537926	39.73932068005033
10	16.089079820592072	26.71876711828507	31.472014820077487	25.72013824104537
11	37.63051395524097	21.052477814702552	22.154422285890714	19.162585944165755
12	24.883321768980753	23.48251901478356	28.62359858306248	23.010560633173203
13	29.936755717714707	19.52332734642927	25.012665456006218	25.527251479849806
14	23.466517049396938	19.822185629438305	24.856563295740887	31.85473402542387
15	25.180520097074165	27.530352275632193	22.00449515791151	25.284632469382128
16	25.73235550922437	25.684947196834134	23.98853967126253	24.59415762267897
17	24.130764608433235	25.84795476954846	25.190479826567913	24.830800795450394
18	24.922363908596243	24.70597218579543	26.474554551098393	23.897109354509933
19	25.771397648839855	24.974552891143475	25.067576764615072	24.18647269540159
20	25.796695361753976	24.49548990249425	25.01160308486022	24.696211650891563
21	26.608479713690976	24.28766354705806	24.20791931291146	24.8959374263395
22	25.930222135166808	24.82382898480477	24.561888099119226	24.68406078090919
23	24.52019003163874	24.54993642372673	25.277328667753384	25.652544876881144
24	24.905897155833248	24.996929083406094	25.150109723019927	24.947064037740734
25	24.952375893470734	24.673304273055944	25.209204118016153	25.16511571545717
26	24.364220667766663	25.519084501664935	25.611510791366904	24.505184039201495
27	25.311590137211876	25.127733530757308	24.764336200628794	24.79634013140203
28	24.311832490629552	25.063061687244577	25.434426800968087	25.190679021157784
29	24.54940523815373	24.999186622091344	25.215578344892155	25.235829794862774
30	24.48938126840475	25.205950606381535	25.489935693346567	24.81473243186715
31	25.05310195775083	24.910412233203747	24.626626340828583	25.409859468216844
32	24.69461809417256	25.26577538154064	24.685853532218065	25.353752992068735
33	24.37484437922666	24.879337877183254	25.372410885320356	25.373406858269732
34	24.882657787014505	25.02222679632021	25.553478767516673	24.54163664914861
35	25.327592102598494	24.835183076427644	25.400563720689345	24.436661100284514
36	24.187003880974594	25.34671478322649	25.20017396327516	25.26610737252376
37	25.205220226218657	25.05901139725045	24.75975472506167	24.976013651469227
38	24.559232171254227	24.982255081951973	25.255085271884013	25.203427474909784
39	25.09645998014694	24.54323020586761	24.97687682802535	25.383432985960102
40	25.482432697127944	24.5732421907421	25.445780892590957	24.498544219539
41	24.260241091851945	24.95582859969523	26.01169272242566	24.772237586027163
42	25.24705109009239	25.32765850079512	25.373207663679853	24.052082745432635
43	24.392506299528904	24.485397376607253	25.718013498753372	25.404082825110468
44	24.06695594147663	24.903971608131123	25.50733202086231	25.52174042952993
45	24.11748496910824	25.158077506614916	25.54942847752255	25.175009046754287
46	23.98926736699244	24.597011674825918	25.79291277046547	25.62080818771617
47	24.451401499935262	24.351206621228165	26.10889968228463	25.088492196551943
48	24.591900083993718	25.212324833257526	25.290674705275006	24.905100377473747
49	23.93601869773217	25.08152038590632	25.664297357683765	25.318163558677746
50	23.602566954281524	25.696633279440128	25.355213752394484	25.345586013883864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	15.5
2	22.0
3	59.5
4	97.0
5	121.0
6	145.0
7	160.5
8	176.0
9	224.0
10	272.0
11	429.0
12	586.0
13	972.0
14	1358.0
15	1819.5
16	2281.0
17	2795.5
18	3310.0
19	3823.0
20	4336.0
21	5214.5
22	6093.0
23	7036.0
24	7979.0
25	9593.0
26	11207.0
27	13942.0
28	16677.0
29	19516.5
30	22356.0
31	25143.5
32	27931.0
33	32665.0
34	37399.0
35	43123.5
36	48848.0
37	55337.0
38	61826.0
39	65002.0
40	68178.0
41	72459.5
42	76741.0
43	78445.0
44	80149.0
45	87187.0
46	94225.0
47	99839.0
48	105453.0
49	108394.5
50	111336.0
51	107342.5
52	103349.0
53	101323.0
54	99297.0
55	99987.5
56	100678.0
57	98415.5
58	96153.0
59	88433.5
60	80714.0
61	71369.0
62	62024.0
63	54779.0
64	47534.0
65	41527.5
66	35521.0
67	31423.5
68	27326.0
69	24708.5
70	22091.0
71	17689.0
72	13287.0
73	11958.5
74	10630.0
75	8201.5
76	5773.0
77	5150.5
78	4528.0
79	3945.0
80	3362.0
81	2710.0
82	2058.0
83	1687.5
84	1317.0
85	1090.0
86	863.0
87	617.0
88	371.0
89	250.5
90	130.0
91	89.0
92	48.0
93	30.0
94	12.0
95	7.5
96	3.0
97	3.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014607603257495527
2	0.0035191044211239225
3	9.295747527497153E-4
4	2.655927864999186E-4
5	0.0
6	5.311855729998372E-4
7	1.9919458987493902E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.655927864999186E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1506065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.299690456051636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3255533216655	49.03905691463552
2	11.995937431553271	14.467026277056508
3	3.121753884142466	5.6472237868130275
4	1.2070822326872002	2.911467399441515
5	0.6056075449910355	1.8258973750404406
6	0.3441338955144145	1.2450700424972647
7	0.24135236387242673	1.0187430982640573
8	0.17607927960053416	0.8494020844509423
9	0.12756396374545234	0.6922860774477994
>10	0.7111440754824692	7.9759231855395365
>50	0.07137483458813734	2.989318228686305
>100	0.06544131035344154	8.141904299527477
>500	0.005757854187203916	2.2959725437397447
>1k	0.0012180076165239054	0.9007086868598354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	6.639819662497967E-5	0.0	0.0	0.0	0.0
11	6.639819662497967E-5	0.0	0.0	0.0	0.0
12	6.639819662497967E-5	0.0	0.0	0.0	1.3279639324995934E-4
13	6.639819662497967E-5	0.0	0.0	0.0	1.99194589874939E-4
14	6.639819662497967E-5	0.0	0.0	0.0	3.3199098312489833E-4
15	6.639819662497967E-5	0.0	0.0	0.0	4.6478737637485764E-4
16	6.639819662497967E-5	0.0	0.0	0.0	4.6478737637485764E-4
17	6.639819662497967E-5	0.0	0.0	6.639819662497967E-5	4.6478737637485764E-4
18	6.639819662497967E-5	0.0	0.0	1.99194589874939E-4	4.6478737637485764E-4
19	6.639819662497967E-5	0.0	0.0	1.99194589874939E-4	4.6478737637485764E-4
20	6.639819662497967E-5	0.0	0.0	5.311855729998373E-4	4.6478737637485764E-4
21	6.639819662497967E-5	0.0	0.0	5.311855729998373E-4	4.6478737637485764E-4
22	6.639819662497967E-5	0.0	0.0	6.639819662497967E-4	5.311855729998373E-4
23	6.639819662497967E-5	0.0	0.0	0.0012615657358746137	5.311855729998373E-4
24	6.639819662497967E-5	0.0	0.0	0.0018591495054994306	5.311855729998373E-4
25	6.639819662497967E-5	0.0	0.0	0.0025231314717492274	5.311855729998373E-4
26	6.639819662497967E-5	0.0	0.0	0.0030543170447490647	5.311855729998373E-4
27	6.639819662497967E-5	0.0	0.0	0.004581475567123597	5.311855729998373E-4
28	6.639819662497967E-5	0.0	0.0	0.01195167539249634	5.311855729998373E-4
29	6.639819662497967E-5	0.0	0.0	0.026957667829741745	5.311855729998373E-4
30	6.639819662497967E-5	0.0	0.0	0.05716884729410749	5.311855729998373E-4
31	6.639819662497967E-5	0.0	0.0	0.12814851948621075	5.311855729998373E-4
32	6.639819662497967E-5	0.0	0.0	0.21187664543031012	5.311855729998373E-4
33	6.639819662497967E-5	0.0	0.0	0.29919027399215836	5.311855729998373E-4
34	6.639819662497967E-5	0.0	0.0	0.39752600319375325	5.311855729998373E-4
35	6.639819662497967E-5	0.0	0.0	0.502767144844346	5.311855729998373E-4
36	6.639819662497967E-5	0.0	0.0	0.6546198205256745	5.311855729998373E-4
37	6.639819662497967E-5	0.0	0.0	0.8774521683991062	5.311855729998373E-4
38	6.639819662497967E-5	0.0	0.0	1.1718617722342661	5.311855729998373E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	45	0.0013984161	24.444315	4
CGCAATA	435	0.0	19.724033	36
TAGGACG	820	0.0	18.243805	4
TTAGGAC	560	0.0	17.285622	3
TACACGG	115	4.2745105E-6	17.2173	5
TAGCGGC	500	0.0	17.15991	30
TCTAGCG	500	0.0	17.15991	28
AGGACGT	815	0.0	17.006044	5
GTATAGG	220	1.8189894E-12	17.002731	1
CAATACG	495	0.0	16.888802	38
GACGTGA	365	0.0	16.876625	7
ATACGAA	510	0.0	16.82344	40
GTCCTAA	460	0.0	16.741821	1
GGACGTG	805	0.0	16.397429	6
GTATCAA	2970	0.0	16.373001	1
AATACGA	525	0.0	16.34277	39
TGTAGGA	925	0.0	16.173962	2
CGAATGC	545	0.0	16.146706	43
GCAATAC	545	0.0	16.146706	37
GTATATC	260	0.0	16.076838	3
>>END_MODULE