FastQCFastQC Report
Thu 2 Feb 2017
SRR4062427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770713
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG73420.9526243880666344No Hit
TCCATGTACTCTGCGTTGATACCAC62550.8115861546386268No Hit
GAGTACATGGAAGCAGTGGTATCAA35430.459704195984757No Hit
CATGTACTCTGCGTTGATACCACTG30550.3963862034246211No Hit
GTATCAACGCAGAGTACTTTTTTTT26940.34954645892829106No Hit
GCGTTGATACCACTGCTTCCATGTA19620.2545694700880873No Hit
GCTTCCATGTACTCTGCGTTGATAC19540.25353147021005223No Hit
CCCATGTACTCTGCGTTGATACCAC18950.24587622110954405No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17570.22797072321344003No Hit
TATCAACGCAGAGTACTTTTTTTTT17140.2223914738690018No Hit
GTACATGGGAAGCAGTGGTATCAAC17130.22226172388424745No Hit
CATGGAAGCAGTGGTATCAACGCAG16150.20954622537831852No Hit
GTATCAACGCAGAGTACATGGAAGC15000.19462497713156518No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13780.17879547899153123No Hit
ACTCTGCGTTGATACCACTGCTTCC12690.16465273065330413No Hit
CAGTGGTATCAACGCAGAGTACATG12530.16257673089723412No Hit
ACGCAGAGTACATGGAAGCAGTGGT12470.16179823098870788No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11440.14843398255900705No Hit
GGTATCAACGCAGAGTACTTTTTTT9520.1235219854861667No Hit
GTATCAACGCAGAGTACATGGGAAG8950.11612623635516722No Hit
GTACATGGGGTGGTATCAACGCAAA8710.11301223672106218No Hit
TATCAACGCAGAGTACATGGAAGCA7900.10250248795595766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT307.700507E-419.0063716
CGCAAGA1450.015.7171512
ACGCGGG456.752988E-414.7788914
ACCGACC456.756316E-414.7779298
AATCGTA456.756316E-414.77792913
CGCGGTC400.005277813414.2492210
CAAGACG1600.014.2464444
GACCATA1152.5465852E-1114.0427111
GCGCAAG1700.013.9634721
AGAGCGA1500.013.934381515
CGACCAT1101.8553692E-1013.81742710
GCTCGTA500.001498231813.3001379
CTCGTAG500.001498965713.29927310
GAGCGAA1600.013.0668816
AAGACGG1750.013.0261665
GGTTCTA957.366725E-813.000133513
TTAGAAC957.398012E-812.9959123
CCGACCA1251.0186341E-1012.9201339
CGAAAGC1700.012.85808619
CGGACCA1700.012.8530729
Produced by FastQC (version 0.11.5)