##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062427_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	770713
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1419412933219	32.0	32.0	32.0	32.0	32.0
2	30.87108690264729	32.0	32.0	32.0	32.0	32.0
3	30.864892638375114	32.0	32.0	32.0	32.0	32.0
4	30.90240076396791	32.0	32.0	32.0	32.0	32.0
5	30.75044536682267	32.0	32.0	32.0	32.0	32.0
6	34.3832723724655	36.0	36.0	36.0	32.0	36.0
7	34.24709068096684	36.0	36.0	36.0	32.0	36.0
8	34.25767308972341	36.0	36.0	36.0	32.0	36.0
9	34.367335181838115	36.0	36.0	36.0	32.0	36.0
10	34.1166919462887	36.0	36.0	36.0	32.0	36.0
11	34.39912263060309	36.0	36.0	36.0	32.0	36.0
12	34.196646481894035	36.0	36.0	36.0	32.0	36.0
13	34.29483089035737	36.0	36.0	36.0	32.0	36.0
14	34.2056978408305	36.0	36.0	36.0	32.0	36.0
15	34.16148293852576	36.0	36.0	36.0	32.0	36.0
16	34.15787459144973	36.0	36.0	36.0	32.0	36.0
17	34.07554303612369	36.0	36.0	36.0	32.0	36.0
18	34.10656236497892	36.0	36.0	36.0	32.0	36.0
19	34.07544572363513	36.0	36.0	36.0	32.0	36.0
20	34.05866515810684	36.0	36.0	36.0	32.0	36.0
21	34.003421507097976	36.0	36.0	36.0	32.0	36.0
22	33.967762318787926	36.0	36.0	36.0	32.0	36.0
23	33.89777777201111	36.0	36.0	36.0	32.0	36.0
24	33.881337151442885	36.0	36.0	36.0	32.0	36.0
25	33.3584953153768	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	22.0
5	85.0
6	126.0
7	31.0
8	105.0
9	139.0
10	87.0
11	34.0
12	64.0
13	45.0
14	174.0
15	212.0
16	307.0
17	422.0
18	609.0
19	779.0
20	1206.0
21	1627.0
22	2395.0
23	3297.0
24	4679.0
25	6231.0
26	8655.0
27	11147.0
28	15220.0
29	20455.0
30	26597.0
31	36885.0
32	53407.0
33	76888.0
34	171272.0
35	327506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.39288295571182	16.821361513617603	11.566754081257939	25.21900144941264
2	15.611015021545544	20.18352314086154	37.68387671874534	26.521585118847586
3	20.347225826542793	24.106633103233506	27.48027715783464	28.065863912389062
4	12.75985263067737	15.34854869248009	35.11376480863141	36.77783386821113
5	14.154357363516004	36.87975390046987	33.64281820305807	15.323070532956052
6	34.50981308471874	33.89736340282132	17.26477220065191	14.328051311808023
7	29.692691407330273	30.732287461876933	20.601711496839144	18.973309633953647
8	28.383385170803926	32.98278649671768	20.011947508879466	18.62188082359893
9	27.1277134596952	15.344992843765503	18.45035703246151	39.076936664077785
10	15.960447023459249	27.14707529069125	31.808787443021135	25.08369024282836
11	36.76585595252633	21.73482086950196	21.353280313442646	20.146042864529072
12	24.185254284853084	24.059099271393084	29.035921048528756	22.71972539522508
13	30.42048705204385	19.891032952528924	24.93524073299436	24.75323926243287
14	23.660210874208182	19.738537807178453	25.063917022194275	31.53733429641909
15	25.535355897196077	26.82397718932471	23.81819008463072	23.82247682884849
16	25.45060843179433	25.93719799233095	25.060661325352534	23.551532250522182
17	23.593495660352602	25.95240353199621	26.753689122496144	23.70041168515504
18	24.92526124459354	24.462342191115543	28.097094770430868	22.515301793860054
19	24.554943755700435	26.97790504600625	25.58745345456152	22.879697743731793
20	24.62946483667093	24.113723019363054	27.789909907569193	23.466902236396823
21	25.07135980542841	25.471780233886065	24.517111412242055	24.939748548443465
22	24.044079426135774	26.07963405759434	25.512267387462312	24.36401912880757
23	23.067977312742414	25.537717890578808	25.6636282720132	25.73067652466557
24	23.25282858048656	26.80797875381345	25.788286532474753	24.15090613322523
25	24.699164598734555	25.225350465771935	25.206121945198717	24.869362990294793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	123.0
2	334.5
3	546.0
4	546.0
5	546.0
6	1499.5
7	2453.0
8	2453.0
9	2453.0
10	2511.0
11	2569.0
12	2569.0
13	2569.0
14	2420.5
15	2272.0
16	2272.0
17	2272.0
18	3753.5
19	5235.0
20	5235.0
21	5235.0
22	8462.5
23	11690.0
24	11690.0
25	11690.0
26	17124.5
27	22559.0
28	22559.0
29	22559.0
30	29180.0
31	35801.0
32	35801.0
33	35801.0
34	41415.0
35	47029.0
36	47029.0
37	47029.0
38	53341.5
39	59654.0
40	59654.0
41	59654.0
42	71471.5
43	83289.0
44	83289.0
45	83289.0
46	106081.5
47	128874.0
48	128874.0
49	128874.0
50	117033.0
51	105192.0
52	105192.0
53	105192.0
54	92539.5
55	79887.0
56	79887.0
57	79887.0
58	72525.0
59	65163.0
60	65163.0
61	65163.0
62	57056.0
63	48949.0
64	48949.0
65	48949.0
66	40609.0
67	32269.0
68	32269.0
69	32269.0
70	24725.0
71	17181.0
72	17181.0
73	17181.0
74	13542.5
75	9904.0
76	9904.0
77	9904.0
78	7878.5
79	5853.0
80	5853.0
81	5853.0
82	4095.5
83	2338.0
84	2338.0
85	2338.0
86	1726.5
87	1115.0
88	1115.0
89	1115.0
90	729.5
91	344.0
92	344.0
93	344.0
94	205.5
95	67.0
96	67.0
97	67.0
98	212.0
99	357.0
100	357.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007265999146245101
2	0.002594999695087536
3	0.004281749496894434
4	0.017126997987577736
5	0.0380167455330324
6	0.04722899445059315
7	0.06617249222473216
8	0.08797048966346746
9	0.09796123848955449
10	0.11807248612648287
11	0.1218352356843598
12	0.1329937343732362
13	0.1218352356843598
14	0.1252087352879736
15	0.11651548630943036
16	0.11223373681253593
17	0.12326248551665796
18	0.13390198426651684
19	0.13429123422078
20	0.1230029855471492
21	0.1326044844189731
22	0.1547917318119715
23	0.14506048295539326
24	0.1387027337024288
25	0.13273423440372745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	770713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.74589723891473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63127134254114	44.948625665043494
2	12.21002620257518	13.613177319464246
3	3.3146268349337134	5.543305407766919
4	1.2797259755233599	2.8535789090198054
5	0.6418013863655447	1.7888897066063336
6	0.3834744038631273	1.2826274826904789
7	0.24768524267389685	0.9665205259986299
8	0.17182306679607584	0.7662744819911382
9	0.14118431845121782	0.7083401857315023
>10	0.7999674932144424	8.577194292031455
>50	0.09738047223458993	3.8753641948614357
>100	0.0726263519239522	7.917723055590893
>500	0.00420345445190704	1.5228543480202406
>1k	0.0037364039572507016	3.865456843542913
>5k	4.670504946563377E-4	1.7700675816405547
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7342	0.9526243880666344	No Hit
TCCATGTACTCTGCGTTGATACCAC	6255	0.8115861546386268	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3543	0.459704195984757	No Hit
CATGTACTCTGCGTTGATACCACTG	3055	0.3963862034246211	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2694	0.34954645892829106	No Hit
GCGTTGATACCACTGCTTCCATGTA	1962	0.2545694700880873	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1954	0.25353147021005223	No Hit
CCCATGTACTCTGCGTTGATACCAC	1895	0.24587622110954405	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1757	0.22797072321344003	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1714	0.2223914738690018	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1713	0.22226172388424745	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1615	0.20954622537831852	No Hit
GTATCAACGCAGAGTACATGGAAGC	1500	0.19462497713156518	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1378	0.17879547899153123	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1269	0.16465273065330413	No Hit
CAGTGGTATCAACGCAGAGTACATG	1253	0.16257673089723412	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1247	0.16179823098870788	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1144	0.14843398255900705	No Hit
GGTATCAACGCAGAGTACTTTTTTT	952	0.1235219854861667	No Hit
GTATCAACGCAGAGTACATGGGAAG	895	0.11612623635516722	No Hit
GTACATGGGGTGGTATCAACGCAAA	871	0.11301223672106218	No Hit
TATCAACGCAGAGTACATGGAAGCA	790	0.10250248795595766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2974998475437678E-4	0.0
4	0.0	0.0	0.0	1.2974998475437678E-4	0.0
5	0.0	0.0	0.0	1.2974998475437678E-4	0.0
6	0.0	0.0	0.0	1.2974998475437678E-4	0.0
7	0.0	0.0	0.0	1.2974998475437678E-4	0.0
8	0.0	0.0	0.0	1.2974998475437678E-4	0.0
9	0.0	0.0	0.0	1.2974998475437678E-4	0.0
10	0.0	0.0	0.0	2.5949996950875356E-4	0.0
11	0.0	0.0	0.0	2.5949996950875356E-4	0.0
12	0.0	0.0	0.0	2.5949996950875356E-4	0.0
13	0.0	0.0	0.0	2.5949996950875356E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAAT	30	7.700507E-4	19.00637	16
CGCAAGA	145	0.0	15.717151	2
ACGCGGG	45	6.752988E-4	14.77889	14
ACCGACC	45	6.756316E-4	14.777929	8
AATCGTA	45	6.756316E-4	14.777929	13
CGCGGTC	40	0.0052778134	14.24922	10
CAAGACG	160	0.0	14.246444	4
GACCATA	115	2.5465852E-11	14.04271	11
GCGCAAG	170	0.0	13.963472	1
AGAGCGA	150	0.0	13.9343815	15
CGACCAT	110	1.8553692E-10	13.817427	10
GCTCGTA	50	0.0014982318	13.300137	9
CTCGTAG	50	0.0014989657	13.299273	10
GAGCGAA	160	0.0	13.06688	16
AAGACGG	175	0.0	13.026166	5
GGTTCTA	95	7.366725E-8	13.0001335	13
TTAGAAC	95	7.398012E-8	12.995912	3
CCGACCA	125	1.0186341E-10	12.920133	9
CGAAAGC	170	0.0	12.858086	19
CGGACCA	170	0.0	12.853072	9
>>END_MODULE