FastQCFastQC Report
Thu 2 Feb 2017
SRR4062427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770713
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29200.37886995548278024No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27180.35266045856239614No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA13520.17542197938791743No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC11250.1459687328486739No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG8270.10730323739186962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGGG450.001396770224.44742
TAGGACG3950.022.8352414
TCGGACT500.002579729521.99980523
ATAAGGT602.8716424E-421.9998054
CGCAATA1800.021.99980536
GTCCTAC3750.021.712111
TGGTCGG1850.021.40521637
AGGACGT4550.021.2745385
TCAATAC1057.737799E-820.9521963
GTATAGA956.843147E-720.847331
GATATAC1502.1827873E-1120.5384831
TTGCGCT755.469709E-520.53315214
GATTGCG755.469709E-520.53315212
TAAGGCG654.930228E-420.3075125
TACGAAT1850.020.21603841
ATACGAA1850.020.21603840
CAATACG1850.020.21603838
CTAGCGG1750.020.11410729
CTCGACG550.004482391319.99982513
TGTAGGA4200.019.907172
Produced by FastQC (version 0.11.5)