##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	770713
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180357668807975	32.0	32.0	32.0	32.0	32.0
2	31.415312833700742	32.0	32.0	32.0	32.0	32.0
3	31.505703160579877	32.0	32.0	32.0	32.0	32.0
4	31.618423459835242	32.0	32.0	32.0	32.0	32.0
5	31.55018664535307	32.0	32.0	32.0	32.0	32.0
6	35.163113895834115	36.0	36.0	36.0	36.0	36.0
7	35.17794691409124	36.0	36.0	36.0	36.0	36.0
8	35.10823484228241	36.0	36.0	36.0	36.0	36.0
9	35.230759050385814	36.0	36.0	36.0	36.0	36.0
10	35.12387101294516	36.0	36.0	36.0	36.0	36.0
11	35.23723227712521	36.0	36.0	36.0	36.0	36.0
12	35.157807121457665	36.0	36.0	36.0	36.0	36.0
13	35.184556378314625	36.0	36.0	36.0	36.0	36.0
14	35.14472183549518	36.0	36.0	36.0	36.0	36.0
15	35.11726025122192	36.0	36.0	36.0	36.0	36.0
16	35.117727351167034	36.0	36.0	36.0	36.0	36.0
17	35.09359644900242	36.0	36.0	36.0	36.0	36.0
18	35.1031395603811	36.0	36.0	36.0	36.0	36.0
19	35.074843683705865	36.0	36.0	36.0	36.0	36.0
20	35.053492026214684	36.0	36.0	36.0	36.0	36.0
21	35.015602435666715	36.0	36.0	36.0	36.0	36.0
22	34.999880630014026	36.0	36.0	36.0	36.0	36.0
23	34.93682862492264	36.0	36.0	36.0	32.0	36.0
24	34.910436180523746	36.0	36.0	36.0	32.0	36.0
25	34.88032639906165	36.0	36.0	36.0	32.0	36.0
26	34.817248443973305	36.0	36.0	36.0	32.0	36.0
27	34.7884919548522	36.0	36.0	36.0	32.0	36.0
28	34.74302885769411	36.0	36.0	36.0	32.0	36.0
29	34.70268699243428	36.0	36.0	36.0	32.0	36.0
30	34.68472699954458	36.0	36.0	36.0	32.0	36.0
31	34.65959312999781	36.0	36.0	36.0	32.0	36.0
32	34.62395599918517	36.0	36.0	36.0	32.0	36.0
33	34.573405405124866	36.0	36.0	36.0	32.0	36.0
34	34.55312418500791	36.0	36.0	36.0	32.0	36.0
35	34.48304492074222	36.0	36.0	36.0	32.0	36.0
36	34.44450787777033	36.0	36.0	36.0	32.0	36.0
37	34.387009172026424	36.0	36.0	36.0	32.0	36.0
38	34.323670419468726	36.0	36.0	36.0	32.0	36.0
39	34.19367520724316	36.0	36.0	36.0	32.0	36.0
40	34.13067250714598	36.0	36.0	36.0	32.0	36.0
41	34.1249310703206	36.0	36.0	36.0	32.0	36.0
42	33.98796309391434	36.0	36.0	36.0	32.0	36.0
43	34.0128374634916	36.0	36.0	36.0	32.0	36.0
44	34.00225635223488	36.0	36.0	36.0	32.0	36.0
45	33.832097032228596	36.0	36.0	36.0	32.0	36.0
46	33.90754275586372	36.0	36.0	36.0	32.0	36.0
47	33.81898709376902	36.0	36.0	36.0	32.0	36.0
48	33.805364642869655	36.0	36.0	36.0	32.0	36.0
49	33.82346606324274	36.0	36.0	36.0	32.0	36.0
50	33.26645327119174	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	19.0
22	77.0
23	243.0
24	685.0
25	1641.0
26	3709.0
27	7155.0
28	11484.0
29	16024.0
30	21783.0
31	29580.0
32	42510.0
33	60820.0
34	127196.0
35	447784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.790436567774286	17.585082831344845	11.939107347242805	25.685373253638065
2	15.124305807857997	20.673690766595733	37.299268178751234	26.90273524679504
3	19.75876820234822	24.327406771150276	27.92330717999354	27.990517846507966
4	12.545023303127497	15.803568648211005	35.129464702768345	36.52194334589315
5	13.96460160915931	37.065288894828555	33.551399807710524	15.418709688301613
6	34.671017633091566	34.07001336430045	17.05686963968289	14.202099362925095
7	29.936214824733494	30.49816273783203	20.52660397139269	19.03901846604179
8	28.493745401984917	33.13126935707585	19.78920817476804	18.585777066171193
9	27.097505816043068	15.048662731782128	18.513376574678254	39.34045487749655
10	15.90929438065791	27.302900041909243	31.87515975466873	24.912645822764116
11	37.05957989549936	21.55666246709216	21.275364500144672	20.108393137263807
12	24.1562034116461	23.93783418730448	29.084367332586837	22.82159506846258
13	30.699105892855055	19.68813293664438	25.01761356043041	24.595147610070157
14	23.68728696674378	19.491691459726255	25.119597048447346	31.701424525082615
15	25.558930496825667	26.873557342357014	23.64109597217122	23.926416188646098
16	25.56788324577372	25.859561211501557	25.045899057106862	23.526656485617863
17	23.877240944424187	25.979579947399355	26.58421487635475	23.5589642318217
18	25.152423794590206	24.366657886917697	27.88314197373082	22.597776344761282
19	24.77575958884825	26.739136358151477	25.461099008320865	23.024005044679406
20	24.64977235365175	24.166194160472187	27.713039743717832	23.470993742158235
21	25.298522277423636	25.33524152310912	24.384303884844293	24.981932314622952
22	24.19422015717913	25.85450096209614	25.51183125235983	24.439447628364906
23	23.233421520072973	25.324212774404998	25.571386495362088	25.870979210159945
24	23.43816699601538	26.657653367725732	25.643657236870276	24.260522399388616
25	25.00411956201595	24.85257157982284	25.139448796114767	25.00386006204644
26	23.066044039739825	25.47368475684204	26.393093148811552	25.067178054606575
27	25.023452309744354	25.921711454198903	25.86319421107468	23.191642024982063
28	23.913311440185904	24.607344108637065	26.125678430232785	25.353666020944242
29	23.2816885144016	25.93507570262861	26.924030086426466	23.85920569654333
30	24.607863108576083	25.151515544696924	26.622491121857294	23.6181302248697
31	23.559612981745477	25.428921012101778	25.77768897112155	25.233777035031196
32	23.53521998461165	26.025770941971913	26.023694942215847	24.41531413120059
33	23.215386272192113	25.465770007772026	26.397764148262716	24.92107957177315
34	23.31672101028528	26.11659593129998	27.113854314122115	23.452828744292624
35	24.86943907784091	25.650144736107993	26.047049939471634	23.433366246579464
36	23.481373740938587	26.40048889794255	26.196911171862936	23.921226189255922
37	24.82000433364949	25.948439951058305	25.89887545688213	23.33268025841007
38	24.006990929178563	25.882397208818333	25.327586274008613	24.78302558799449
39	24.02191217742532	25.501062003625215	26.288514661099526	24.18851115784994
40	24.88578757591996	25.71164622888157	25.41698401350438	23.98558218169409
41	23.122615033092732	25.80532571787423	26.753149356504952	24.318909892528087
42	25.218207036860672	25.950515950814374	26.04679043950212	22.78448657282283
43	24.132069914481786	24.749809591897375	25.759913223210194	25.35820727041064
44	23.678463967780484	25.370403768977557	27.007718826593035	23.94341343664892
45	24.125841915213574	25.784176470359267	25.950256450844865	24.13972516358229
46	23.269262707729997	25.11171488182356	27.063676185137897	24.555346225308547
47	24.393775633731362	24.712571346272867	27.138506811225447	23.75514620877032
48	23.677555717887202	27.27435504526328	24.983489314440003	24.06459992240951
49	23.028416544161058	25.42450951262013	27.563438011296032	23.983635931922777
50	22.863634063522998	27.556172012149787	24.732552843925042	24.847641080402173
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	17.0
2	20.0
3	73.0
4	126.0
5	152.0
6	178.0
7	173.0
8	168.0
9	211.0
10	254.0
11	403.0
12	552.0
13	920.0
14	1288.0
15	1827.5
16	2367.0
17	2774.5
18	3182.0
19	3594.0
20	4006.0
21	4445.0
22	4884.0
23	5240.5
24	5597.0
25	5709.5
26	5822.0
27	7320.0
28	8818.0
29	9983.0
30	11148.0
31	12754.0
32	14360.0
33	16753.5
34	19147.0
35	21887.0
36	24627.0
37	27532.0
38	30437.0
39	32018.0
40	33599.0
41	36133.5
42	38668.0
43	42027.5
44	45387.0
45	51816.0
46	58245.0
47	63429.5
48	68614.0
49	66700.0
50	64786.0
51	58929.5
52	53073.0
53	50188.0
54	47303.0
55	46865.5
56	46428.0
57	44753.0
58	43078.0
59	39280.5
60	35483.0
61	31385.5
62	27288.0
63	23802.5
64	20317.0
65	17380.0
66	14443.0
67	12867.5
68	11292.0
69	10339.0
70	9386.0
71	7462.0
72	5538.0
73	4901.5
74	4265.0
75	3237.0
76	2209.0
77	1904.5
78	1600.0
79	1395.0
80	1190.0
81	936.5
82	683.0
83	546.5
84	410.0
85	329.0
86	248.0
87	185.5
88	123.0
89	84.0
90	45.0
91	27.5
92	10.0
93	6.5
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014402248307735826
2	0.004281749496894434
3	2.5949996950875356E-4
4	1.2974998475437678E-4
5	0.0
6	3.8924995426313036E-4
7	1.2974998475437678E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2974998475437678E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	770713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.72423737526186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.44772915412328	50.27298967985142
2	11.886951090871245	14.674259816021292
3	3.174941961158053	5.879126137895973
4	1.1949875695826977	2.950387856216387
5	0.575501599908503	1.7761198681297712
6	0.3527491028254814	1.30639016140264
7	0.2265267287821131	0.9787532705432099
8	0.16332425546964233	0.8064852090996891
9	0.11875549852191551	0.6597083322345846
>10	0.7207209725811806	8.555637996536763
>50	0.07609301817868015	3.3606687535724697
>100	0.05812581575325495	6.635029189250166
>500	0.0027477658335864725	1.085724932456146
>1k	8.454664103342993E-4	1.0587187967894287
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2920	0.37886995548278024	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2718	0.35266045856239614	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1352	0.17542197938791743	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1125	0.1459687328486739	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	827	0.10730323739186962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.5949996950875356E-4	0.0
6	0.0	0.0	0.0	2.5949996950875356E-4	0.0
7	0.0	0.0	0.0	2.5949996950875356E-4	0.0
8	0.0	0.0	0.0	2.5949996950875356E-4	0.0
9	0.0	0.0	0.0	2.5949996950875356E-4	0.0
10	0.0	0.0	0.0	3.8924995426313036E-4	0.0
11	0.0	0.0	0.0	3.8924995426313036E-4	0.0
12	0.0	0.0	0.0	3.8924995426313036E-4	0.0
13	0.0	0.0	0.0	3.8924995426313036E-4	0.0
14	0.0	0.0	0.0	3.8924995426313036E-4	0.0
15	0.0	0.0	0.0	9.082498932806376E-4	0.0
16	0.0	0.0	0.0	0.0011677498627893912	0.0
17	0.0	0.0	0.0	0.0011677498627893912	0.0
18	0.0	0.0	0.0	0.001297499847543768	0.0
19	0.0	0.0	0.0	0.0015569998170525214	0.0
20	0.0	0.0	0.0	0.0018164997865612752	0.0
21	0.0	0.0	0.0	0.002594999695087536	0.0
22	0.0	0.0	0.0	0.004670999451157565	0.0
23	0.0	0.0	0.0	0.0071362491614907235	0.0
24	0.0	0.0	0.0	0.01089899871936765	0.0
25	0.0	0.0	0.0	0.013104748460192056	0.0
26	0.0	0.0	0.0	0.017516247941840868	0.0
27	0.0	0.0	0.0	0.02387399719480533	0.0
28	0.0	0.0	0.0	0.033215996097120455	0.0
29	0.0	0.0	0.0	0.05021324409994382	0.0
30	0.0	0.0	0.0	0.08083424050197674	0.0
31	0.0	0.0	0.0	0.13623748399209562	0.0
32	0.0	0.0	0.0	0.20721072565273974	0.0
33	0.0	0.0	0.0	0.27195596804517375	0.0
34	0.0	0.0	0.0	0.35694220805929056	0.0
35	0.0	0.0	0.0	0.44426394779898615	0.0
36	0.0	0.0	0.0	0.5708999329192579	0.0
37	0.0	0.0	0.0	0.7600754106911393	0.0
38	0.0	0.0	0.0	0.9837643844076849	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGGG	45	0.0013967702	24.4474	2
TAGGACG	395	0.0	22.835241	4
TCGGACT	50	0.0025797295	21.999805	23
ATAAGGT	60	2.8716424E-4	21.999805	4
CGCAATA	180	0.0	21.999805	36
GTCCTAC	375	0.0	21.71211	1
TGGTCGG	185	0.0	21.405216	37
AGGACGT	455	0.0	21.274538	5
TCAATAC	105	7.737799E-8	20.952196	3
GTATAGA	95	6.843147E-7	20.84733	1
GATATAC	150	2.1827873E-11	20.538483	1
TTGCGCT	75	5.469709E-5	20.533152	14
GATTGCG	75	5.469709E-5	20.533152	12
TAAGGCG	65	4.930228E-4	20.307512	5
TACGAAT	185	0.0	20.216038	41
ATACGAA	185	0.0	20.216038	40
CAATACG	185	0.0	20.216038	38
CTAGCGG	175	0.0	20.114107	29
CTCGACG	55	0.0044823913	19.999825	13
TGTAGGA	420	0.0	19.90717	2
>>END_MODULE