FastQCFastQC Report
Thu 2 Feb 2017
SRR4062426_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062426_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292237
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG30360.2349414232838094No Hit
GTATCAACGCAGAGTACTTTTTTTT24700.1911414082710834No Hit
TCCATGTACTCTGCGTTGATACCAC24250.18765907492201508No Hit
TATCAACGCAGAGTACTTTTTTTTT16670.12900110428659758No Hit
GAGTACATGGAAGCAGTGGTATCAA13950.10795233382111795No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13420.10385091898777081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG453.5577395E-516.8780121
CGTCGTA1151.8189894E-1214.87035410
ATAATAC802.003555E-613.059653
CGACCCG1102.746674E-912.9537275
CCGTCGT1251.0186341E-1012.9206859
TACCGTC1251.0186341E-1012.91968257
ACCGTCG1301.9826984E-1012.4227728
ATACCGT1301.9826984E-1012.4227726
GTCCTAA2500.012.1521691
CGAACGA550.00306407412.09295616
AGGACGG550.003069414512.0901455
CGTTATT550.0030774412.085932
TAGGAGT658.0286677E-411.6911164
CCGGCAC658.0365E-411.6897563
TAGACTG907.462955E-611.6103775
ATTAGCT907.475841E-611.6085783
GATATAC3050.011.5171691
CCAACGA752.0700372E-411.40193119
TTCCGGT752.0730197E-411.40016311
GTCTTAG1001.9435574E-611.3926581
Produced by FastQC (version 0.11.5)