##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062426_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1292237
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169785418619032	32.0	32.0	32.0	32.0	32.0
2	30.764934760419337	32.0	32.0	32.0	32.0	32.0
3	30.75025246916781	32.0	32.0	32.0	32.0	32.0
4	30.832472681094877	32.0	32.0	32.0	32.0	32.0
5	30.592563902751586	32.0	32.0	32.0	32.0	32.0
6	34.25285377217956	36.0	36.0	36.0	32.0	36.0
7	34.14065608708	36.0	36.0	36.0	32.0	36.0
8	34.12589641064294	36.0	36.0	36.0	32.0	36.0
9	34.33178356601769	36.0	36.0	36.0	32.0	36.0
10	33.93338064147676	36.0	36.0	36.0	32.0	36.0
11	34.3173605151377	36.0	36.0	36.0	32.0	36.0
12	34.070656543652596	36.0	36.0	36.0	32.0	36.0
13	34.20272829210122	36.0	36.0	36.0	32.0	36.0
14	34.09303401775371	36.0	36.0	36.0	32.0	36.0
15	34.02133664335567	36.0	36.0	36.0	32.0	36.0
16	34.027183094122826	36.0	36.0	36.0	32.0	36.0
17	33.946228903831106	36.0	36.0	36.0	32.0	36.0
18	33.95151431200314	36.0	36.0	36.0	32.0	36.0
19	33.94530956782695	36.0	36.0	36.0	32.0	36.0
20	33.933948648738586	36.0	36.0	36.0	32.0	36.0
21	33.914984635171415	36.0	36.0	36.0	32.0	36.0
22	33.86192703041315	36.0	36.0	36.0	32.0	36.0
23	33.81027396677235	36.0	36.0	36.0	32.0	36.0
24	33.78636581370136	36.0	36.0	36.0	32.0	36.0
25	33.290852219832736	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	45.0
5	131.0
6	244.0
7	58.0
8	223.0
9	220.0
10	162.0
11	48.0
12	88.0
13	77.0
14	248.0
15	313.0
16	422.0
17	618.0
18	845.0
19	1216.0
20	1889.0
21	2648.0
22	3966.0
23	5850.0
24	8207.0
25	11432.0
26	15853.0
27	21125.0
28	28211.0
29	37893.0
30	48501.0
31	66291.0
32	94807.0
33	135243.0
34	291291.0
35	514061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.115264329778185	17.662564612956878	11.378226584744262	25.843944472520676
2	16.19869708352874	19.82226906819339	38.160686840357904	25.81834700791996
3	19.31144107966633	23.756420366559688	28.508865271553358	28.423273282220624
4	12.355480704187634	15.357859883811825	36.305863122096646	35.980796289903886
5	14.08589830928346	36.91359075989348	34.36048491979935	14.640026011023718
6	34.0671983549201	35.56827182220006	16.936085146511058	13.428444676368779
7	29.715633607908764	30.711353514640365	20.900825275085243	18.67218760236563
8	27.675764351955483	34.25469439476523	19.520224063773902	18.549317189505377
9	26.981612495168218	14.41602998146299	18.744533983613376	39.857823539755415
10	15.306394426262024	27.62160009421799	32.72936192105524	24.342643558464744
11	37.079467402210504	21.279350407351306	22.435507552366822	19.205674638071365
12	23.816114221507583	23.954280400627674	29.811813479532727	22.41779189833201
13	29.70522262297241	19.96156930503202	25.36828770333983	24.964920368655736
14	22.649178935688656	20.166498654481973	25.587777907779518	31.596544502049852
15	24.708149199521507	28.02945314582158	22.75041681903546	24.511980835621447
16	25.028627122242074	26.14024268362033	24.912259226145743	23.918870967991857
17	23.33641458514289	26.56010649302147	26.136737845391412	23.96674107644423
18	23.99997210413835	25.48038998414585	27.374518990107816	23.14511892160798
19	25.095757680955476	25.771233873461153	25.590373321286297	23.542635124297075
20	24.769083314414733	25.312931721804375	26.195997728218362	23.721987235562526
21	25.342031608865856	25.05067708930636	24.925611612027364	24.681679689800422
22	24.550395119062753	25.742727732118887	25.471538716707848	24.235338432110513
23	23.70539371174071	25.46008014736824	25.90444778370285	24.930078357188194
24	24.097250098028123	25.85315464575813	25.883609232776884	24.165986023436858
25	24.343956339437163	25.52109607044086	25.90962734627869	24.22532024384328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	171.0
1	171.0
2	414.5
3	658.0
4	658.0
5	658.0
6	1758.5
7	2859.0
8	2859.0
9	2859.0
10	3207.5
11	3556.0
12	3556.0
13	3556.0
14	4124.0
15	4692.0
16	4692.0
17	4692.0
18	7593.5
19	10495.0
20	10495.0
21	10495.0
22	16678.0
23	22861.0
24	22861.0
25	22861.0
26	33625.5
27	44390.0
28	44390.0
29	44390.0
30	55918.5
31	67447.0
32	67447.0
33	67447.0
34	78562.5
35	89678.0
36	89678.0
37	89678.0
38	99853.0
39	110028.0
40	110028.0
41	110028.0
42	123357.5
43	136687.0
44	136687.0
45	136687.0
46	155514.0
47	174341.0
48	174341.0
49	174341.0
50	170595.0
51	166849.0
52	166849.0
53	166849.0
54	154218.0
55	141587.0
56	141587.0
57	141587.0
58	128146.5
59	114706.0
60	114706.0
61	114706.0
62	100521.5
63	86337.0
64	86337.0
65	86337.0
66	70972.0
67	55607.0
68	55607.0
69	55607.0
70	42253.0
71	28899.0
72	28899.0
73	28899.0
74	22074.0
75	15249.0
76	15249.0
77	15249.0
78	11922.5
79	8596.0
80	8596.0
81	8596.0
82	6076.5
83	3557.0
84	3557.0
85	3557.0
86	2605.5
87	1654.0
88	1654.0
89	1654.0
90	1095.0
91	536.0
92	536.0
93	536.0
94	350.5
95	165.0
96	165.0
97	165.0
98	398.5
99	632.0
100	632.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00928622226418219
2	0.004565725946556244
3	0.004643111132091095
4	0.015090111179296057
5	0.0369127335001242
6	0.048597896515886786
7	0.07042051883671493
8	0.09100497818898545
9	0.10106505230851616
10	0.1248223042677156
11	0.1224233635161352
12	0.13635269691240848
13	0.12397106722683224
14	0.1281498672457142
15	0.11677424497209103
16	0.11630993385888191
17	0.1266021635350172
18	0.13333467467654928
19	0.13465022283064174
20	0.12536400056645955
21	0.13256082282120074
22	0.1542286747709592
23	0.14424598583696335
24	0.13859686729291917
25	0.13434068208850236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1292237.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.970535542706045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91033954564764	47.71326053949029
2	12.046874610960609	14.208212948483515
3	3.2124042091752703	5.6831158978412635
4	1.289194520945151	3.0409796527543174
5	0.6482952923674923	1.9115160290363107
6	0.394188339272867	1.3947298482966524
7	0.25100117690825724	1.0361171676890604
8	0.17645958834945893	0.8324733141290439
9	0.13466785552078564	0.7147292004403645
>10	0.7739652096775568	8.539232969639894
>50	0.08400115745159582	3.4342889951438096
>100	0.07215655580632813	8.195970681615933
>500	0.005398560297872337	2.1737502424253177
>1k	0.0010533776190970413	1.1216225130142423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3036	0.2349414232838094	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2470	0.1911414082710834	No Hit
TCCATGTACTCTGCGTTGATACCAC	2425	0.18765907492201508	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1667	0.12900110428659758	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1395	0.10795233382111795	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1342	0.10385091898777081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.738518553485158E-5
2	0.0	0.0	0.0	0.0	7.738518553485158E-5
3	0.0	0.0	0.0	0.0	7.738518553485158E-5
4	0.0	0.0	0.0	7.738518553485158E-5	7.738518553485158E-5
5	0.0	0.0	0.0	7.738518553485158E-5	7.738518553485158E-5
6	0.0	0.0	0.0	7.738518553485158E-5	7.738518553485158E-5
7	0.0	0.0	0.0	1.5477037106970316E-4	7.738518553485158E-5
8	0.0	0.0	0.0	1.5477037106970316E-4	7.738518553485158E-5
9	0.0	0.0	0.0	1.5477037106970316E-4	7.738518553485158E-5
10	0.0	0.0	0.0	1.5477037106970316E-4	7.738518553485158E-5
11	0.0	0.0	0.0	1.5477037106970316E-4	7.738518553485158E-5
12	0.0	0.0	0.0	2.3215555660455474E-4	1.5477037106970316E-4
13	0.0	0.0	0.0	2.3215555660455474E-4	2.3215555660455474E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATTAG	45	3.5577395E-5	16.878012	1
CGTCGTA	115	1.8189894E-12	14.870354	10
ATAATAC	80	2.003555E-6	13.05965	3
CGACCCG	110	2.746674E-9	12.953727	5
CCGTCGT	125	1.0186341E-10	12.920685	9
TACCGTC	125	1.0186341E-10	12.9196825	7
ACCGTCG	130	1.9826984E-10	12.422772	8
ATACCGT	130	1.9826984E-10	12.422772	6
GTCCTAA	250	0.0	12.152169	1
CGAACGA	55	0.003064074	12.092956	16
AGGACGG	55	0.0030694145	12.090145	5
CGTTATT	55	0.00307744	12.08593	2
TAGGAGT	65	8.0286677E-4	11.691116	4
CCGGCAC	65	8.0365E-4	11.689756	3
TAGACTG	90	7.462955E-6	11.610377	5
ATTAGCT	90	7.475841E-6	11.608578	3
GATATAC	305	0.0	11.517169	1
CCAACGA	75	2.0700372E-4	11.401931	19
TTCCGGT	75	2.0730197E-4	11.400163	11
GTCTTAG	100	1.9435574E-6	11.392658	1
>>END_MODULE