##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1292237
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243721546434593	32.0	32.0	32.0	32.0	32.0
2	31.352063901590807	32.0	32.0	32.0	32.0	32.0
3	31.47724449926755	32.0	32.0	32.0	32.0	32.0
4	31.59669549780729	32.0	32.0	32.0	32.0	32.0
5	31.51768677107992	32.0	32.0	32.0	32.0	32.0
6	35.11629523067363	36.0	36.0	36.0	36.0	36.0
7	35.13878491329377	36.0	36.0	36.0	36.0	36.0
8	35.07123228943298	36.0	36.0	36.0	36.0	36.0
9	35.21999757010517	36.0	36.0	36.0	36.0	36.0
10	35.08608637579639	36.0	36.0	36.0	36.0	36.0
11	35.23045849948578	36.0	36.0	36.0	36.0	36.0
12	35.13918499470299	36.0	36.0	36.0	36.0	36.0
13	35.18506434965103	36.0	36.0	36.0	36.0	36.0
14	35.145100318285266	36.0	36.0	36.0	36.0	36.0
15	35.10048466341701	36.0	36.0	36.0	36.0	36.0
16	35.1127239043612	36.0	36.0	36.0	36.0	36.0
17	35.09344106382962	36.0	36.0	36.0	36.0	36.0
18	35.10763582841228	36.0	36.0	36.0	36.0	36.0
19	35.09025588959301	36.0	36.0	36.0	36.0	36.0
20	35.08363017000752	36.0	36.0	36.0	36.0	36.0
21	35.062545028504836	36.0	36.0	36.0	36.0	36.0
22	35.049583009927744	36.0	36.0	36.0	36.0	36.0
23	35.01357336154281	36.0	36.0	36.0	36.0	36.0
24	34.995062051311024	36.0	36.0	36.0	36.0	36.0
25	34.967398395186024	36.0	36.0	36.0	36.0	36.0
26	34.91425644057553	36.0	36.0	36.0	32.0	36.0
27	34.8907561074323	36.0	36.0	36.0	32.0	36.0
28	34.87216741201498	36.0	36.0	36.0	32.0	36.0
29	34.84734147064354	36.0	36.0	36.0	32.0	36.0
30	34.83439338139985	36.0	36.0	36.0	32.0	36.0
31	34.82377690779632	36.0	36.0	36.0	32.0	36.0
32	34.79076283994345	36.0	36.0	36.0	32.0	36.0
33	34.772958830307445	36.0	36.0	36.0	32.0	36.0
34	34.75725505460686	36.0	36.0	36.0	32.0	36.0
35	34.72566410031597	36.0	36.0	36.0	32.0	36.0
36	34.688691780222975	36.0	36.0	36.0	32.0	36.0
37	34.665545097377645	36.0	36.0	36.0	32.0	36.0
38	34.62040090169218	36.0	36.0	36.0	32.0	36.0
39	34.590986792670385	36.0	36.0	36.0	32.0	36.0
40	34.571724846138906	36.0	36.0	36.0	32.0	36.0
41	34.55754246318593	36.0	36.0	36.0	32.0	36.0
42	34.515122226031295	36.0	36.0	36.0	32.0	36.0
43	34.520167740128166	36.0	36.0	36.0	32.0	36.0
44	34.491279850368	36.0	36.0	36.0	32.0	36.0
45	34.42577329081276	36.0	36.0	36.0	32.0	36.0
46	34.4282387828239	36.0	36.0	36.0	32.0	36.0
47	34.395153520600324	36.0	36.0	36.0	32.0	36.0
48	34.36563107231878	36.0	36.0	36.0	32.0	36.0
49	34.35709780790985	36.0	36.0	36.0	32.0	36.0
50	33.832193320575094	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	5.0
21	33.0
22	91.0
23	314.0
24	831.0
25	1952.0
26	4234.0
27	7771.0
28	13089.0
29	19741.0
30	28902.0
31	41866.0
32	63142.0
33	102977.0
34	229238.0
35	778047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78377834517357	18.175893899910452	11.886185116360425	26.154142638555548
2	15.68697555241361	20.336126259776986	37.72308006621302	26.25381812159639
3	18.748336212073376	24.091417021092187	28.788715958125167	28.371530808709274
4	12.188640465054371	15.923175023757272	36.47878560882068	35.40939890236768
5	13.862405938234193	37.12619057200078	34.163264295376386	14.84813919438864
6	34.30269046323761	35.81480400949985	16.647410563591183	13.235094963671356
7	29.84377466654698	30.685029669503095	20.792873747519803	18.678321916430125
8	27.586503095020497	34.46651040018201	19.34459390963113	18.602392595166368
9	27.095494092801864	14.237249049516459	18.538859357842252	40.12839749983942
10	15.292163898727557	27.671549413923298	32.70367587369809	24.33261081365106
11	37.313356605638134	21.12723904361197	22.37329530109415	19.18610904965575
12	23.857233618910463	23.96626934532907	29.730769201005696	22.44572783475477
13	29.853424720078436	19.868878541629748	25.29845531431154	24.979241423980277
14	22.694443821063786	20.041911816485676	25.576732441494865	31.686911920955673
15	24.743371378470048	28.09028065285238	22.523035635104087	24.643312333573487
16	25.000909275930034	26.22483337034925	24.766819089687107	24.00743826403361
17	23.378451476006337	26.54242217178428	26.09180823641484	23.987318115794547
18	24.176447509241726	25.37359632946588	27.264580723195515	23.18537543809688
19	25.157382121081508	25.704804923555045	25.55591582658599	23.58189712877746
20	24.863627956791206	25.349374766393474	26.075789503009123	23.711207773806198
21	25.595459656394297	24.98597393512181	24.78245089716515	24.636115511318742
22	24.764110608193388	25.72817524958657	25.368101981292906	24.139612160927136
23	23.8233389076462	25.347207981198494	25.875516642844925	24.95393646831038
24	24.14827930170704	25.894785554043104	25.732586205162058	24.2243489390878
25	24.4859108661956	25.288704780934147	25.83109754634792	24.394286806522334
26	23.540031743403105	25.88201699842985	26.427350400893957	24.150600857273087
27	24.51098366630889	25.98548099148995	25.437439107532136	24.066096234669025
28	23.793932537142954	25.55963031549166	25.97596261366916	24.67047453369622
29	23.914885582133927	25.67888088640087	25.88673749474748	24.519496036717722
30	24.21575918349343	25.55668967844134	26.10713050315074	24.120420634914495
31	24.21181253903115	25.418170196333957	25.69141728645751	24.678599978177377
32	23.970989841646695	25.806411672162305	25.693351916095885	24.529246570095115
33	23.785497551919654	25.393329551777267	26.1665623256415	24.65461057066157
34	23.939726226690613	25.87249862060907	26.464495289950683	23.723279862749635
35	24.610346244535638	25.57727413779361	25.995308910052877	23.817070707617873
36	23.78124136671524	25.81801944999253	26.068437910383313	24.332301272908914
37	24.68804097081263	25.583000641523192	25.472030285466214	24.25692810219797
38	24.02531424189216	25.833651257470574	25.734907760728102	24.406126739909165
39	24.38995323613238	25.39627018882759	25.587643752655282	24.626132822384747
40	25.029077483464718	25.37351894428034	25.882249153986457	23.715154418268476
41	23.989484900989524	25.377697744299226	26.554881186655386	24.07793616805586
42	24.75134205258014	25.974492295144003	25.85129507977252	23.422870572503342
43	23.975787723149857	25.360750388667096	26.060312465902154	24.603149422280897
44	23.6688006921331	25.75812331638856	26.162151370066017	24.410924621412324
45	23.733417322054702	25.89045198365315	26.058068295521643	24.318062398770504
46	23.892134337586683	25.294276514292658	26.093510710496602	24.720078437624057
47	24.397072673201585	25.085259128163024	26.2853485854375	24.232319613197888
48	24.231468376157007	26.19070650352838	25.373209403538205	24.204615716776413
49	23.763597544413294	25.719198568064527	26.076795510421075	24.440408377101104
50	23.509696750673445	26.52686774949177	25.327242603330504	24.63619289650428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	34.0
2	37.0
3	95.5
4	154.0
5	183.5
6	213.0
7	201.5
8	190.0
9	242.0
10	294.0
11	445.0
12	596.0
13	1073.5
14	1551.0
15	2071.0
16	2591.0
17	3104.5
18	3618.0
19	4004.0
20	4390.0
21	5411.5
22	6433.0
23	7104.0
24	7775.0
25	9548.5
26	11322.0
27	14837.5
28	18353.0
29	20618.5
30	22884.0
31	25394.5
32	27905.0
33	32327.0
34	36749.0
35	41869.5
36	46990.0
37	52821.0
38	58652.0
39	60668.5
40	62685.0
41	66032.5
42	69380.0
43	70509.5
44	71639.0
45	77954.5
46	84270.0
47	89065.5
48	93861.0
49	94187.5
50	94514.0
51	91342.5
52	88171.0
53	85810.0
54	83449.0
55	82952.0
56	82455.0
57	79946.5
58	77438.0
59	70397.5
60	63357.0
61	56085.5
62	48814.0
63	42562.0
64	36310.0
65	30855.0
66	25400.0
67	22370.5
68	19341.0
69	17187.0
70	15033.0
71	11887.5
72	8742.0
73	7759.5
74	6777.0
75	5043.0
76	3309.0
77	2945.0
78	2581.0
79	2116.5
80	1652.0
81	1324.0
82	996.0
83	814.5
84	633.0
85	512.0
86	391.0
87	292.0
88	193.0
89	127.0
90	61.0
91	42.5
92	24.0
93	16.5
94	9.0
95	10.5
96	12.0
97	10.5
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013465022283064173
2	0.0034049481635334694
3	3.869259276742579E-4
4	7.738518553485158E-5
5	7.738518553485158E-5
6	4.643111132091095E-4
7	7.738518553485158E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1292237.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.48612807694952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51104056204304	50.93309320245248
2	11.806115457013	14.754368850763356
3	3.1177173145487576	5.844422502738504
4	1.2377729394351724	3.0937455369491387
5	0.619069804455585	1.934163754489189
6	0.35013218834992743	1.3127042859057707
7	0.2538144745113237	1.110191863346875
8	0.16382306478773484	0.8189335206627839
9	0.12257870149449017	0.6893521596981791
>10	0.6872352068474054	8.06779293493991
>50	0.06934285215505295	3.0472297096945424
>100	0.05799027211520992	6.962106452183637
>500	0.003117742817906808	1.2637303337628376
>1k	2.494194254325446E-4	0.16816489241291258
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.738518553485158E-5
2	0.0	0.0	0.0	0.0	7.738518553485158E-5
3	0.0	0.0	0.0	0.0	7.738518553485158E-5
4	0.0	0.0	0.0	0.0	7.738518553485158E-5
5	0.0	0.0	0.0	0.0	7.738518553485158E-5
6	0.0	0.0	0.0	0.0	7.738518553485158E-5
7	0.0	0.0	0.0	0.0	7.738518553485158E-5
8	0.0	0.0	0.0	0.0	7.738518553485158E-5
9	0.0	0.0	0.0	0.0	7.738518553485158E-5
10	0.0	0.0	0.0	0.0	1.5477037106970316E-4
11	0.0	0.0	0.0	0.0	1.5477037106970316E-4
12	0.0	0.0	0.0	1.5477037106970316E-4	3.8692592767425787E-4
13	0.0	0.0	0.0	1.5477037106970316E-4	3.8692592767425787E-4
14	0.0	0.0	0.0	2.3215555660455474E-4	3.8692592767425787E-4
15	0.0	0.0	0.0	2.3215555660455474E-4	6.964666698136642E-4
16	0.0	0.0	0.0	2.3215555660455474E-4	7.738518553485157E-4
17	0.0	0.0	0.0	3.095407421394063E-4	7.738518553485157E-4
18	0.0	7.738518553485158E-5	0.0	3.095407421394063E-4	7.738518553485157E-4
19	0.0	7.738518553485158E-5	0.0	4.643111132091095E-4	7.738518553485157E-4
20	0.0	7.738518553485158E-5	0.0	5.41696298743961E-4	0.0010060074119530706
21	0.0	7.738518553485158E-5	0.0	6.964666698136642E-4	0.0011607777830227738
22	0.0	7.738518553485158E-5	0.0	0.0013929333396273285	0.0011607777830227738
23	0.0	7.738518553485158E-5	0.0	0.002708481493719805	0.0011607777830227738
24	0.0	7.738518553485158E-5	0.0	0.004024029647812282	0.0011607777830227738
25	0.0	7.738518553485158E-5	0.0	0.005262192616369907	0.0011607777830227738
26	0.0	7.738518553485158E-5	0.0	0.007583748182415455	0.0011607777830227738
27	0.0	7.738518553485158E-5	0.0	0.00982791856292615	0.0011607777830227738
28	0.0	7.738518553485158E-5	0.0	0.016328274147853684	0.0012381629685576253
29	0.0	7.738518553485158E-5	0.0	0.026697889009523794	0.0012381629685576253
30	0.0	7.738518553485158E-5	0.0	0.04728234836179431	0.0012381629685576253
31	0.0	7.738518553485158E-5	0.0	0.08218306703801238	0.0012381629685576253
32	0.0	7.738518553485158E-5	0.0	0.12203643758846094	0.0012381629685576253
33	0.0	7.738518553485158E-5	0.0	0.16490783037476872	0.0012381629685576253
34	0.0	7.738518553485158E-5	0.0	0.21420219356046918	0.0015477037106970315
35	0.0	7.738518553485158E-5	0.0	0.2648121049002621	0.0015477037106970315
36	0.0	7.738518553485158E-5	0.0	0.3416555941363697	0.0015477037106970315
37	0.0	1.5477037106970316E-4	0.0	0.4551022761304621	0.0015477037106970315
38	0.0	1.5477037106970316E-4	0.0	0.5946277656497996	0.0015477037106970315
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	145	0.0	22.75836	8
TACCGTC	155	0.0	22.70942	7
ATACCGT	160	0.0	22.000599	6
ACGATTA	100	1.1236825E-6	19.799774	40
ACGTATG	100	1.1236825E-6	19.799774	22
TGTAGGA	890	0.0	19.529377	2
TCTAGCG	295	0.0	19.38961	28
TATAGGA	205	0.0	19.318348	2
CGTCGTA	160	5.2750693E-11	19.249779	10
GATATAC	310	0.0	19.166264	1
TAGCGGC	300	0.0	19.06645	30
GACGTGG	455	0.0	18.856926	7
TAGGACG	605	0.0	18.54596	4
TAGGACC	775	0.0	18.452116	4
GTCCTAC	780	0.0	18.33809	1
CGCAATA	300	0.0	18.333124	36
GTAGGAC	940	0.0	18.255817	3
CTGTAGG	935	0.0	18.12235	1
CCGTCGT	170	1.2732926E-10	18.11744	9
TAACGCC	270	0.0	17.926414	4
>>END_MODULE