FastQCFastQC Report
Thu 2 Feb 2017
SRR4062425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416121
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG204934.924769478108531No Hit
TCCATGTACTCTGCGTTGATACCAC174564.194933685154078No Hit
GAGTACATGGAAGCAGTGGTATCAA89092.1409638062006002No Hit
CATGTACTCTGCGTTGATACCACTG78481.8859898923630387No Hit
GCTTCCATGTACTCTGCGTTGATAC53571.2873659344277266No Hit
CCCATGTACTCTGCGTTGATACCAC51241.2313726055642469No Hit
GCGTTGATACCACTGCTTCCATGTA48161.1573556729893468No Hit
GTACATGGGAAGCAGTGGTATCAAC47381.1386111251294695No Hit
CATGGAAGCAGTGGTATCAACGCAG46431.1157812270950036No Hit
GTATCAACGCAGAGTACTTTTTTTT46021.1059283237327604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41510.9975463867480853No Hit
GTATCAACGCAGAGTACATGGAAGC38120.9160796979724648No Hit
ACTCTGCGTTGATACCACTGCTTCC31830.764921741512685No Hit
TATCAACGCAGAGTACTTTTTTTTT31810.7644411120803805No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31000.7449756200720464No Hit
ACGCAGAGTACATGGAAGCAGTGGT30710.7380064933036304No Hit
CAGTGGTATCAACGCAGAGTACATG30660.7368049197228691No Hit
TATCAACGCAGAGTACATGGAAGCA23320.5604139180671007No Hit
GTATCAACGCAGAGTACATGGGAAG21570.5183588427404529No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21060.506102792216687No Hit
GTACATGGGGTGGTATCAACGCAAA18560.44602411317861873No Hit
ATACCACTGCTTCCATGTACTCTGC17630.4236748445764573No Hit
GGTATCAACGCAGAGTACATGGAAG17560.4219926415633914No Hit
GAGTACATGGGAAGCAGTGGTATCA16890.4058915555811891No Hit
AAGCAGTGGTATCAACGCAGAGTAC15510.3727281247521755No Hit
GCGTTGATACCACTGCTTCCCATGT15390.3698443481583482No Hit
GGTATCAACGCAGAGTACTTTTTTT14880.3575882976345823No Hit
GTACTCTGCGTTGATACCACTGCTT14370.34533224711081634No Hit
AAAAAGTACTCTGCGTTGATACCAC13930.3347583996001163No Hit
TATCAACGCAGAGTACATGGGAAGC13340.3205798313471322No Hit
CCACTGCTTCCATGTACTCTGCGTT13330.3203395166309799No Hit
GTGGTATCAACGCAGAGTACATGGA12830.30832378082336626No Hit
GCTTCCCATGTACTCTGCGTTGATA12530.3011143393387981No Hit
GAAGCAGTGGTATCAACGCAGAGTA12390.29774993331266625No Hit
ACATGGAAGCAGTGGTATCAACGCA11670.28044727374970263No Hit
ACGCAGAGTACATGGGAAGCAGTGG11120.26722996436132757No Hit
CTGCTTCCATGTACTCTGCGTTGAT11000.2643461877675003No Hit
GTACATGGGTGGTATCAACGCAAAA10980.26386555833519576No Hit
GGTATCAACGCAGAGTACATGGGAA10460.25136919309527755No Hit
TGGAAGCAGTGGTATCAACGCAGAG10400.24992730479836395No Hit
GTTGATACCACTGCTTCCATGTACT9980.23983408671996845No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9740.2340665335323139No Hit
GTACATGGGAGTGGTATCAACGCAA9200.2210895388600912No Hit
GATACCACTGCTTCCATGTACTCTG8970.21556230038858887No Hit
CCCCATGTACTCTGCGTTGATACCA8740.2100350619170866No Hit
GCAGAGTACATGGAAGCAGTGGTAT8480.2037868792971275No Hit
CTTCCATGTACTCTGCGTTGATACC7960.19129051405720932No Hit
GCAGTGGTATCAACGCAGAGTACAT7680.18456170200494568No Hit
GTATCAACGCAGAGTACATGGGGTG7320.17591037222346387No Hit
CCATGTACTCTGCGTTGATACCACT7320.17591037222346387No Hit
GTGGTATCAACGCAGAGTACATGGG6070.14587103270442972No Hit
AAAGTACTCTGCGTTGATACCACTG6060.14563071798827745No Hit
ATCAACGCAGAGTACATGGAAGCAG5840.14034379423292745No Hit
CTGCTTCCCATGTACTCTGCGTTGA5730.13770033235525242No Hit
CCATAGGGTCTTCTCGTCTTATTAT5210.12520396711533424No Hit
TATCAACGCAGAGTACATGGGGTGG5070.12183956108920242No Hit
TGATACCACTGCTTCCATGTACTCT5030.12087830222459334No Hit
GTATTAGAGGCACTGCCTGCCCAGT4970.1194364139276797No Hit
CACTGCTTCCATGTACTCTGCGTTG4810.11559137846924332No Hit
GTATCAACGCAGAGTACATGGGAGT4770.11463011960463423No Hit
ATCAACGCAGAGTACTTTTTTTTTT4600.1105447694300456No Hit
TCCATGTACTCTGCGTTGATACCAA4560.10958351056543648No Hit
GTCCTACAGTGGACATTTCTAAATT4470.10742067812006605No Hit
GTATCAACGCAGAGTACATGGGCAG4350.10453690152623876No Hit
GATACCACTGCTTCCCATGTACTCT4300.10333532794547738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGA250.006017751519.0038516
CGACCTC250.006021938319.00156414
TAGGACC307.7116914E-418.9992774
CCTAAGG250.0060387118.992423
TTAGATT307.730444E-418.9924183
TAGGGTC1000.018.0493134
AGGGTCT1000.017.1014085
AATCGTA453.5263307E-516.8902813
CATGGCA402.7558097E-416.6243694
GTACATA402.778672E-416.6063791
AAAAAGT2350.015.7482981
GGTCTTC1055.456968E-1215.382227
AAATCGT508.683368E-515.20125112
CTACACT456.7495287E-414.7772174
CTTCTCG1101.0913936E-1114.68302710
CTGTAGA851.729859E-814.5131381
GTCCTAC1900.014.4837581
TTGAACT400.00526340314.2528919
GACCTCG400.00526340314.25289115
AAGAAGG400.0052676114.2511747
Produced by FastQC (version 0.11.5)