##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062425_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416121
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.67225879011153	32.0	32.0	32.0	32.0	32.0
2	30.73743694742635	32.0	32.0	32.0	32.0	32.0
3	30.735110700973994	32.0	32.0	32.0	32.0	32.0
4	30.63790099514324	32.0	32.0	32.0	32.0	32.0
5	30.686434955217354	32.0	32.0	32.0	32.0	32.0
6	34.16146265148839	36.0	36.0	36.0	32.0	36.0
7	33.88594423256697	36.0	36.0	36.0	32.0	36.0
8	33.88466575827704	36.0	36.0	36.0	32.0	36.0
9	33.88509832476611	36.0	36.0	36.0	32.0	36.0
10	33.82657207879439	36.0	36.0	36.0	32.0	36.0
11	34.12755664818646	36.0	36.0	36.0	32.0	36.0
12	33.981983605730065	36.0	36.0	36.0	32.0	36.0
13	33.988409621239974	36.0	36.0	36.0	32.0	36.0
14	33.92131615563742	36.0	36.0	36.0	32.0	36.0
15	33.98007310373665	36.0	36.0	36.0	32.0	36.0
16	33.84604718339137	36.0	36.0	36.0	32.0	36.0
17	33.77718019518361	36.0	36.0	36.0	32.0	36.0
18	33.82617315636558	36.0	36.0	36.0	32.0	36.0
19	33.81549837667409	36.0	36.0	36.0	32.0	36.0
20	33.71262925927795	36.0	36.0	36.0	27.0	36.0
21	33.49983057812511	36.0	36.0	36.0	27.0	36.0
22	33.466626293794356	36.0	36.0	36.0	27.0	36.0
23	33.25893430035975	36.0	36.0	36.0	21.0	36.0
24	33.27346613124548	36.0	36.0	36.0	21.0	36.0
25	32.64995277815827	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	17.0
5	42.0
6	57.0
7	21.0
8	60.0
9	76.0
10	57.0
11	20.0
12	49.0
13	27.0
14	167.0
15	209.0
16	305.0
17	414.0
18	609.0
19	878.0
20	1242.0
21	1774.0
22	2371.0
23	3127.0
24	3811.0
25	4704.0
26	5910.0
27	7120.0
28	9064.0
29	11828.0
30	15566.0
31	21249.0
32	30527.0
33	42109.0
34	87009.0
35	165699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.19855653799843	16.9559919535285	13.968126551673585	20.877324956799484
2	10.07577377934041	24.426649876113597	38.958489042481865	26.539087302064136
3	20.55837136478228	32.01635172060764	27.328329764505423	20.09694715010466
4	11.454364969679197	19.066893241453574	34.47918644589245	34.99955534297478
5	9.985960998499941	41.41745836378322	34.508538789953455	14.088041847763375
6	29.626566391936986	32.64682970927381	21.925880689741394	15.800723209047806
7	24.504381450736336	34.28641509796943	23.776223776223777	17.43297967507046
8	29.880360218209105	30.39389245408276	24.077806748318693	15.647940579389438
9	27.877394119882805	18.053624436500794	19.0370117342545	35.0319697093619
10	15.61568353805989	29.774419807745524	33.92413286974097	20.685763784453613
11	32.08990418625691	24.861043604637175	19.506590503322922	23.54246170578299
12	20.85945377494026	27.782924992118748	30.9426461280782	20.414975104862794
13	32.583980385127155	22.85354051823495	25.203860414867073	19.358618681770825
14	26.14082622673109	20.257907097618727	26.270525728145994	27.33074094750419
15	26.9893438106764	23.966922264434213	32.730068065837884	16.313665859051504
16	24.54886675328425	26.681824743756316	29.894615273567197	18.874693229392232
17	21.62301334263749	25.533164093987992	32.99541610019611	19.84840646317841
18	24.831791623752274	22.024468423028424	35.83512210147369	17.308617851745613
19	19.951099891466786	35.556298897100405	27.97413492290773	16.51846628852508
20	20.10159442121018	22.66720247176195	39.29841616655517	17.932786940472695
21	21.285320319380872	29.839683891864837	24.540252289714452	24.334743499039835
22	18.516075924383703	31.363817977885073	27.26745389514425	22.852652202586974
23	18.232973676800107	29.564922296378484	26.51381865705229	25.688285369769115
24	17.16099502583342	34.7532288766373	27.36465429903236	20.721121798496924
25	24.65491019001598	26.282415339891806	24.90807231003215	24.154602160060065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	251.0
2	613.0
3	975.0
4	975.0
5	975.0
6	3185.5
7	5396.0
8	5396.0
9	5396.0
10	5099.5
11	4803.0
12	4803.0
13	4803.0
14	3500.5
15	2198.0
16	2198.0
17	2198.0
18	3408.5
19	4619.0
20	4619.0
21	4619.0
22	7038.0
23	9457.0
24	9457.0
25	9457.0
26	13653.0
27	17849.0
28	17849.0
29	17849.0
30	21310.0
31	24771.0
32	24771.0
33	24771.0
34	25097.5
35	25424.0
36	25424.0
37	25424.0
38	26461.0
39	27498.0
40	27498.0
41	27498.0
42	37713.5
43	47929.0
44	47929.0
45	47929.0
46	91452.5
47	134976.0
48	134976.0
49	134976.0
50	98119.5
51	61263.0
52	61263.0
53	61263.0
54	41192.5
55	21122.0
56	21122.0
57	21122.0
58	16368.0
59	11614.0
60	11614.0
61	11614.0
62	9244.5
63	6875.0
64	6875.0
65	6875.0
66	5529.5
67	4184.0
68	4184.0
69	4184.0
70	3352.5
71	2521.0
72	2521.0
73	2521.0
74	1932.5
75	1344.0
76	1344.0
77	1344.0
78	931.0
79	518.0
80	518.0
81	518.0
82	337.5
83	157.0
84	157.0
85	157.0
86	105.0
87	53.0
88	53.0
89	53.0
90	39.5
91	26.0
92	26.0
93	26.0
94	20.5
95	15.0
96	15.0
97	15.0
98	149.0
99	283.0
100	283.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009131959213786374
2	0.0033644060261318227
3	0.004325664890740914
4	0.01682203013065911
5	0.0329231161128614
6	0.047341999081997786
7	0.06656717637417962
8	0.08963738912479784
9	0.09973060720319331
10	0.1264055406960956
11	0.12568459654763878
12	0.13890190593601381
13	0.12448302296687741
14	0.1309715203029888
15	0.11871546977922287
16	0.12039767279228879
17	0.12880868785761834
18	0.13577781462603425
19	0.13938253536831835
20	0.13145214973529334
21	0.13625844405833878
22	0.15740613907973883
23	0.15187890060823656
24	0.13794064707140472
25	0.13866159121986155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	416121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.006701822210697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74531423970174	18.4412708397745
2	14.444497936601017	7.224185057442386
3	4.597451729410881	3.4490131361815446
4	1.9124951301762814	1.913007818269932
5	1.0338525366421685	1.2926621055973433
6	0.6991252672616817	1.0489690256871742
7	0.4451622098864507	0.7792427051602794
8	0.3405929886548789	0.6813685848022518
9	0.2569384568043579	0.5782665038368987
>10	1.9529312823948128	10.171462869837248
>50	0.2848232258278825	4.864461084582246
>100	0.2300495285532897	11.188783474535972
>500	0.016930051884874134	3.0980338858446292
>1k	0.03386010376974827	19.035226693290532
>5k	0.003983541619970384	6.7832995966749206
>10k+	0.001991770809985192	9.45074661848214
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	20493	4.924769478108531	No Hit
TCCATGTACTCTGCGTTGATACCAC	17456	4.194933685154078	No Hit
GAGTACATGGAAGCAGTGGTATCAA	8909	2.1409638062006002	No Hit
CATGTACTCTGCGTTGATACCACTG	7848	1.8859898923630387	No Hit
GCTTCCATGTACTCTGCGTTGATAC	5357	1.2873659344277266	No Hit
CCCATGTACTCTGCGTTGATACCAC	5124	1.2313726055642469	No Hit
GCGTTGATACCACTGCTTCCATGTA	4816	1.1573556729893468	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4738	1.1386111251294695	No Hit
CATGGAAGCAGTGGTATCAACGCAG	4643	1.1157812270950036	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4602	1.1059283237327604	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4151	0.9975463867480853	No Hit
GTATCAACGCAGAGTACATGGAAGC	3812	0.9160796979724648	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3183	0.764921741512685	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3181	0.7644411120803805	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3100	0.7449756200720464	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	3071	0.7380064933036304	No Hit
CAGTGGTATCAACGCAGAGTACATG	3066	0.7368049197228691	No Hit
TATCAACGCAGAGTACATGGAAGCA	2332	0.5604139180671007	No Hit
GTATCAACGCAGAGTACATGGGAAG	2157	0.5183588427404529	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2106	0.506102792216687	No Hit
GTACATGGGGTGGTATCAACGCAAA	1856	0.44602411317861873	No Hit
ATACCACTGCTTCCATGTACTCTGC	1763	0.4236748445764573	No Hit
GGTATCAACGCAGAGTACATGGAAG	1756	0.4219926415633914	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1689	0.4058915555811891	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1551	0.3727281247521755	No Hit
GCGTTGATACCACTGCTTCCCATGT	1539	0.3698443481583482	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1488	0.3575882976345823	No Hit
GTACTCTGCGTTGATACCACTGCTT	1437	0.34533224711081634	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1393	0.3347583996001163	No Hit
TATCAACGCAGAGTACATGGGAAGC	1334	0.3205798313471322	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1333	0.3203395166309799	No Hit
GTGGTATCAACGCAGAGTACATGGA	1283	0.30832378082336626	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1253	0.3011143393387981	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1239	0.29774993331266625	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1167	0.28044727374970263	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1112	0.26722996436132757	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1100	0.2643461877675003	No Hit
GTACATGGGTGGTATCAACGCAAAA	1098	0.26386555833519576	No Hit
GGTATCAACGCAGAGTACATGGGAA	1046	0.25136919309527755	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1040	0.24992730479836395	No Hit
GTTGATACCACTGCTTCCATGTACT	998	0.23983408671996845	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	974	0.2340665335323139	No Hit
GTACATGGGAGTGGTATCAACGCAA	920	0.2210895388600912	No Hit
GATACCACTGCTTCCATGTACTCTG	897	0.21556230038858887	No Hit
CCCCATGTACTCTGCGTTGATACCA	874	0.2100350619170866	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	848	0.2037868792971275	No Hit
CTTCCATGTACTCTGCGTTGATACC	796	0.19129051405720932	No Hit
GCAGTGGTATCAACGCAGAGTACAT	768	0.18456170200494568	No Hit
GTATCAACGCAGAGTACATGGGGTG	732	0.17591037222346387	No Hit
CCATGTACTCTGCGTTGATACCACT	732	0.17591037222346387	No Hit
GTGGTATCAACGCAGAGTACATGGG	607	0.14587103270442972	No Hit
AAAGTACTCTGCGTTGATACCACTG	606	0.14563071798827745	No Hit
ATCAACGCAGAGTACATGGAAGCAG	584	0.14034379423292745	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	573	0.13770033235525242	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	521	0.12520396711533424	No Hit
TATCAACGCAGAGTACATGGGGTGG	507	0.12183956108920242	No Hit
TGATACCACTGCTTCCATGTACTCT	503	0.12087830222459334	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	497	0.1194364139276797	No Hit
CACTGCTTCCATGTACTCTGCGTTG	481	0.11559137846924332	No Hit
GTATCAACGCAGAGTACATGGGAGT	477	0.11463011960463423	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	460	0.1105447694300456	No Hit
TCCATGTACTCTGCGTTGATACCAA	456	0.10958351056543648	No Hit
GTCCTACAGTGGACATTTCTAAATT	447	0.10742067812006605	No Hit
GTATCAACGCAGAGTACATGGGCAG	435	0.10453690152623876	No Hit
GATACCACTGCTTCCCATGTACTCT	430	0.10333532794547738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.4031471615227303E-4	0.0
11	0.0	0.0	0.0	2.4031471615227303E-4	0.0
12	0.0	0.0	0.0	4.8062943230454605E-4	0.0
13	0.0	0.0	0.0	7.20944148456819E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTCGA	25	0.0060177515	19.00385	16
CGACCTC	25	0.0060219383	19.001564	14
TAGGACC	30	7.7116914E-4	18.999277	4
CCTAAGG	25	0.00603871	18.99242	3
TTAGATT	30	7.730444E-4	18.992418	3
TAGGGTC	100	0.0	18.049313	4
AGGGTCT	100	0.0	17.101408	5
AATCGTA	45	3.5263307E-5	16.89028	13
CATGGCA	40	2.7558097E-4	16.624369	4
GTACATA	40	2.778672E-4	16.606379	1
AAAAAGT	235	0.0	15.748298	1
GGTCTTC	105	5.456968E-12	15.38222	7
AAATCGT	50	8.683368E-5	15.201251	12
CTACACT	45	6.7495287E-4	14.777217	4
CTTCTCG	110	1.0913936E-11	14.683027	10
CTGTAGA	85	1.729859E-8	14.513138	1
GTCCTAC	190	0.0	14.483758	1
TTGAACT	40	0.005263403	14.252891	9
GACCTCG	40	0.005263403	14.252891	15
AAGAAGG	40	0.00526761	14.251174	7
>>END_MODULE