FastQCFastQC Report
Thu 2 Feb 2017
SRR4062424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283154
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28400.22132962995867994No Hit
TATCAACGCAGAGTACTTTTTTTTT18410.1434745946316654No Hit
GCGCAAGACGGACCAGAGCGAAAGC16530.12882319659214717No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14550.11339246886967581No Hit
GATTAAGAGGGACGGCCGGGGGCAT14420.1123793402818368No Hit
GAATAGGACCGCGGTTCTATTTTGT14330.11167794356717899No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14210.11074274794763528No Hit
GAACTACGACGGTATCTGATCGTCT14200.11066481497933997No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14140.11019721716956812No Hit
GAATAACGCCGCCGCATCGCCAGTC14130.1101192842012728No Hit
GTATCTGATCGTCTTCGAACCTCCG13960.10879442374025254No Hit
GTACATGGAAGCAGTGGTATCAACG13780.10739163031093694No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13220.1030273840863996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCA851.022272E-915.6432044
CGAACGA1051.364242E-913.57496916
CGGTCCA2950.013.20477210
CCCTTAC655.483682E-513.1454771
GCGTTAT1403.6379788E-1212.8846551
ATCCCCG1650.012.668982512
ACGCGCG604.0848507E-412.66898213
GTCCTAA1900.012.4920481
CAAGACG3500.012.4826384
ACGAACG1155.311449E-912.39260315
CGCAAGA3450.012.3843862
CGGAATT701.0895463E-412.21556615
TCAGGAC1950.012.174113
TAACGAA1209.977157E-911.87717113
TCGGCGT2000.011.87717113
CGCATCG2100.011.76405413
CATCGCC2100.011.76313715
CGCGTAA1052.7145325E-711.76313710
TAGAGTG1950.011.6903385
ATTTAGA1950.011.6848691
Produced by FastQC (version 0.11.5)