##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062424_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283154
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131990392423667	32.0	32.0	32.0	32.0	32.0
2	30.846283454674964	32.0	32.0	32.0	32.0	32.0
3	30.8671515655954	32.0	32.0	32.0	32.0	32.0
4	30.78021967745103	32.0	32.0	32.0	32.0	32.0
5	30.78208539271202	32.0	32.0	32.0	32.0	32.0
6	34.206223882714	36.0	36.0	36.0	32.0	36.0
7	34.183192352593686	36.0	36.0	36.0	32.0	36.0
8	34.15789920773344	36.0	36.0	36.0	32.0	36.0
9	34.28639040988065	36.0	36.0	36.0	32.0	36.0
10	34.002299801894395	36.0	36.0	36.0	32.0	36.0
11	34.30571544802884	36.0	36.0	36.0	32.0	36.0
12	34.09901617420824	36.0	36.0	36.0	32.0	36.0
13	34.22055341759446	36.0	36.0	36.0	32.0	36.0
14	34.10162926663518	36.0	36.0	36.0	32.0	36.0
15	34.0402765373447	36.0	36.0	36.0	32.0	36.0
16	34.0581426703264	36.0	36.0	36.0	32.0	36.0
17	33.98579204055008	36.0	36.0	36.0	32.0	36.0
18	34.00272141925287	36.0	36.0	36.0	32.0	36.0
19	33.975419162469976	36.0	36.0	36.0	32.0	36.0
20	33.94046388820048	36.0	36.0	36.0	32.0	36.0
21	33.915565084159816	36.0	36.0	36.0	32.0	36.0
22	33.87159842076633	36.0	36.0	36.0	32.0	36.0
23	33.85257965918354	36.0	36.0	36.0	32.0	36.0
24	33.82945850614969	36.0	36.0	36.0	32.0	36.0
25	33.20836703934212	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	36.0
5	122.0
6	208.0
7	40.0
8	201.0
9	231.0
10	142.0
11	50.0
12	91.0
13	87.0
14	348.0
15	563.0
16	897.0
17	1138.0
18	1347.0
19	1593.0
20	2009.0
21	2487.0
22	3315.0
23	4529.0
24	6327.0
25	8941.0
26	12565.0
27	17404.0
28	24217.0
29	33470.0
30	46295.0
31	67886.0
32	104429.0
33	159842.0
34	346323.0
35	436012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72021417965995	17.866387121161935	11.185665239062068	26.227733460116053
2	16.951963513593558	20.172814759162907	36.660281129881504	26.21494059736203
3	19.03169416112608	23.816408140552582	28.531525430069355	28.620372268251987
4	12.790466562585982	15.38248092915005	35.83215313734675	35.99489937091722
5	14.828749422122364	36.05137868155804	33.479871927503545	15.639999968816046
6	34.03499361052007	35.247084580496924	16.917268904712934	13.800652904270075
7	29.881363558734513	30.405101374772777	21.13176030026803	18.581774766224683
8	27.919645259795722	32.53243790291444	19.968800333836427	19.579116503453413
9	27.408422643958534	14.222874700943642	18.963858936665968	39.404843718431856
10	16.214560857756567	26.358131467641023	31.32017705039289	26.10713062420953
11	37.364889801262294	21.06583835229733	22.418403310136473	19.150868536303907
12	24.634309833700225	23.64529790141251	28.758756470053008	22.961635794834258
13	29.33228362184794	19.442081986732436	25.36941469481641	25.856219696603212
14	23.478551269476768	19.857271157490963	24.936972872000506	31.727204701031763
15	25.090445223782197	27.31293618316084	22.34201831140308	25.25460028165388
16	25.66569011051599	25.59110254619787	24.06634860323863	24.676858740047514
17	23.899426031753958	25.720971532548276	25.54790715384783	24.83169528184993
18	24.863265810951376	24.441728878869387	26.741297899884586	23.953707410294648
19	25.551956438106664	24.88491884818184	25.484222625746494	24.078902087964995
20	25.571135748466972	24.23360249935043	25.234138542602174	24.96112320958043
21	26.801028824889073	24.063920740529177	24.3297109083219	24.80533952625985
22	25.780795722843376	24.442623270697965	24.894924779020076	24.881656227438583
23	24.301477388103322	24.18371400276108	25.636675373405144	25.878133235730456
24	24.752112100113468	24.95852277072574	25.371422150011004	24.91794297914979
25	24.672583304655355	24.47164235476261	25.513101740109605	25.342672600472426
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	121.0
2	385.5
3	650.0
4	650.0
5	650.0
6	1658.5
7	2667.0
8	2667.0
9	2667.0
10	2969.5
11	3272.0
12	3272.0
13	3272.0
14	3597.5
15	3923.0
16	3923.0
17	3923.0
18	6538.0
19	9153.0
20	9153.0
21	9153.0
22	14797.0
23	20441.0
24	20441.0
25	20441.0
26	30433.5
27	40426.0
28	40426.0
29	40426.0
30	49624.5
31	58823.0
32	58823.0
33	58823.0
34	71012.0
35	83201.0
36	83201.0
37	83201.0
38	93161.0
39	103121.0
40	103121.0
41	103121.0
42	116606.0
43	130091.0
44	130091.0
45	130091.0
46	147077.5
47	164064.0
48	164064.0
49	164064.0
50	165133.5
51	166203.0
52	166203.0
53	166203.0
54	153739.0
55	141275.0
56	141275.0
57	141275.0
58	130646.0
59	120017.0
60	120017.0
61	120017.0
62	107794.5
63	95572.0
64	95572.0
65	95572.0
66	79729.0
67	63886.0
68	63886.0
69	63886.0
70	49144.5
71	34403.0
72	34403.0
73	34403.0
74	27002.5
75	19602.0
76	19602.0
77	19602.0
78	16322.0
79	13042.0
80	13042.0
81	13042.0
82	9158.5
83	5275.0
84	5275.0
85	5275.0
86	3887.0
87	2499.0
88	2499.0
89	2499.0
90	1663.5
91	828.0
92	828.0
93	828.0
94	467.5
95	107.0
96	107.0
97	107.0
98	299.5
99	492.0
100	492.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00927402322714187
2	0.0035849165415842523
3	0.0036628495098795623
4	0.013638269451679222
5	0.034524304954822255
6	0.04496732270639378
7	0.0649181625899931
8	0.08486900247359241
9	0.09390922679584836
10	0.1157304579185351
11	0.11806844696739441
12	0.13038185595805335
13	0.11939330742841467
14	0.12180922944556927
15	0.11214554137695086
16	0.11214554137695086
17	0.12079610085773025
18	0.12882319659214717
19	0.1299142581482815
20	0.12009470414307247
21	0.13186258235566425
22	0.15033269584165268
23	0.13801928685099374
24	0.1346681692142954
25	0.13116118564100646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1283154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.84024390013896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73143236516552	42.65868576582918
2	11.679182796126874	12.34261735003302
3	3.2738602190402597	5.189747174071489
4	1.3314771475851215	2.8142230890343654
5	0.7567639863126344	1.9993796805800514
6	0.43735528122224787	1.3865975838478468
7	0.29883017202374945	1.105318142211878
8	0.21643413034050962	0.9149145788405597
9	0.15131002550836833	0.7195732787158589
>10	0.9008852465662712	8.862424760799975
>50	0.10027249671245872	3.735523048095434
>100	0.10817605415033688	11.991923838562641
>500	0.010183004925852623	3.5322507718328175
>1k	0.003837074319886495	2.746820937545019
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2840	0.22132962995867994	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1841	0.1434745946316654	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1653	0.12882319659214717	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1455	0.11339246886967581	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1442	0.1123793402818368	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1433	0.11167794356717899	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1421	0.11074274794763528	No Hit
GAACTACGACGGTATCTGATCGTCT	1420	0.11066481497933997	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1414	0.11019721716956812	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1413	0.1101192842012728	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1396	0.10879442374025254	No Hit
GTACATGGAAGCAGTGGTATCAACG	1378	0.10739163031093694	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1322	0.1030273840863996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.558659365906197E-4
2	0.0	0.0	0.0	0.0	1.558659365906197E-4
3	0.0	0.0	0.0	0.0	1.558659365906197E-4
4	0.0	0.0	0.0	0.0	1.558659365906197E-4
5	0.0	0.0	0.0	0.0	1.558659365906197E-4
6	0.0	0.0	0.0	0.0	2.3379890488592952E-4
7	0.0	0.0	0.0	7.793296829530984E-5	2.3379890488592952E-4
8	0.0	0.0	0.0	7.793296829530984E-5	2.3379890488592952E-4
9	0.0	0.0	0.0	7.793296829530984E-5	2.3379890488592952E-4
10	0.0	0.0	0.0	7.793296829530984E-5	2.3379890488592952E-4
11	0.0	0.0	0.0	7.793296829530984E-5	2.3379890488592952E-4
12	0.0	0.0	0.0	2.3379890488592952E-4	3.117318731812394E-4
13	0.0	0.0	0.0	3.117318731812394E-4	4.6759780977185904E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCA	85	1.022272E-9	15.643204	4
CGAACGA	105	1.364242E-9	13.574969	16
CGGTCCA	295	0.0	13.204772	10
CCCTTAC	65	5.483682E-5	13.145477	1
GCGTTAT	140	3.6379788E-12	12.884655	1
ATCCCCG	165	0.0	12.6689825	12
ACGCGCG	60	4.0848507E-4	12.668982	13
GTCCTAA	190	0.0	12.492048	1
CAAGACG	350	0.0	12.482638	4
ACGAACG	115	5.311449E-9	12.392603	15
CGCAAGA	345	0.0	12.384386	2
CGGAATT	70	1.0895463E-4	12.215566	15
TCAGGAC	195	0.0	12.17411	3
TAACGAA	120	9.977157E-9	11.877171	13
TCGGCGT	200	0.0	11.877171	13
CGCATCG	210	0.0	11.764054	13
CATCGCC	210	0.0	11.763137	15
CGCGTAA	105	2.7145325E-7	11.763137	10
TAGAGTG	195	0.0	11.690338	5
ATTTAGA	195	0.0	11.684869	1
>>END_MODULE