FastQCFastQC Report
Thu 2 Feb 2017
SRR4062424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283154
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15960.12438101739931451No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15040.117211184316146No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14510.11308073699649458No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14420.1123793402818368No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13920.1084826918670713No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13280.10349498189617147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG901.0017546E-519.5561962
CGCAATA4500.018.57766336
TACCGTC3450.018.492647
TAGGACG3350.018.3879454
ATAACCG600.00741260918.3332214
ACCGTCG3600.018.333228
ATACCGT3600.018.333226
CGTCGTA3650.017.47934510
ACACGCT4400.016.9998959
TATACCA1053.7748356E-516.76185
CTAGCGG5000.016.71989629
TAACGCC6200.016.6773174
CAATACG4900.016.6121438
ATCGTTT6100.016.59006129
GTCTATA1206.3956704E-616.5031151
TTAGGAC3750.016.4272043
TCTAGCG5100.016.39205628
AATAACG6450.016.372632
CCGTCGT3900.016.3588739
CTTACAC1351.0852E-616.2968313
Produced by FastQC (version 0.11.5)