##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283154
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17825529905218	32.0	32.0	32.0	32.0	32.0
2	31.167019703013043	32.0	32.0	32.0	32.0	32.0
3	31.335621445282484	32.0	32.0	32.0	32.0	32.0
4	31.49662706113218	32.0	32.0	32.0	32.0	32.0
5	31.369890909430982	32.0	32.0	32.0	32.0	32.0
6	34.95379899840549	36.0	36.0	36.0	36.0	36.0
7	34.961629703059806	36.0	36.0	36.0	36.0	36.0
8	34.89194749811792	36.0	36.0	36.0	32.0	36.0
9	35.06378891387939	36.0	36.0	36.0	36.0	36.0
10	34.889862011886336	36.0	36.0	36.0	32.0	36.0
11	35.07749732300254	36.0	36.0	36.0	36.0	36.0
12	34.95191457923211	36.0	36.0	36.0	32.0	36.0
13	35.019153585617936	36.0	36.0	36.0	36.0	36.0
14	34.97148510623043	36.0	36.0	36.0	32.0	36.0
15	34.921084296974485	36.0	36.0	36.0	32.0	36.0
16	34.93935801938037	36.0	36.0	36.0	32.0	36.0
17	34.90883557234751	36.0	36.0	36.0	32.0	36.0
18	34.93165512479406	36.0	36.0	36.0	32.0	36.0
19	34.90993520653016	36.0	36.0	36.0	32.0	36.0
20	34.89057198122751	36.0	36.0	36.0	32.0	36.0
21	34.89172928580669	36.0	36.0	36.0	32.0	36.0
22	34.867761001407466	36.0	36.0	36.0	32.0	36.0
23	34.82535767335799	36.0	36.0	36.0	32.0	36.0
24	34.79900775744766	36.0	36.0	36.0	32.0	36.0
25	34.78311176990447	36.0	36.0	36.0	32.0	36.0
26	34.72945647989251	36.0	36.0	36.0	32.0	36.0
27	34.70805063149084	36.0	36.0	36.0	32.0	36.0
28	34.69868932333921	36.0	36.0	36.0	32.0	36.0
29	34.667920608126536	36.0	36.0	36.0	32.0	36.0
30	34.64703613128276	36.0	36.0	36.0	32.0	36.0
31	34.656063107000406	36.0	36.0	36.0	32.0	36.0
32	34.619235882832456	36.0	36.0	36.0	32.0	36.0
33	34.59799213500484	36.0	36.0	36.0	32.0	36.0
34	34.586753421646975	36.0	36.0	36.0	32.0	36.0
35	34.566512671121316	36.0	36.0	36.0	32.0	36.0
36	34.52356069497504	36.0	36.0	36.0	32.0	36.0
37	34.52581607507751	36.0	36.0	36.0	32.0	36.0
38	34.48445237282509	36.0	36.0	36.0	32.0	36.0
39	34.472301064408484	36.0	36.0	36.0	32.0	36.0
40	34.475850911114335	36.0	36.0	36.0	32.0	36.0
41	34.4294862502864	36.0	36.0	36.0	32.0	36.0
42	34.41829273805015	36.0	36.0	36.0	32.0	36.0
43	34.40475110547916	36.0	36.0	36.0	32.0	36.0
44	34.3631972467841	36.0	36.0	36.0	32.0	36.0
45	34.310598727822224	36.0	36.0	36.0	32.0	36.0
46	34.3350860457903	36.0	36.0	36.0	32.0	36.0
47	34.31274266378003	36.0	36.0	36.0	32.0	36.0
48	34.26928412333983	36.0	36.0	36.0	32.0	36.0
49	34.267592977927826	36.0	36.0	36.0	32.0	36.0
50	33.657058311005535	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	29.0
22	100.0
23	276.0
24	830.0
25	1999.0
26	4154.0
27	7767.0
28	13524.0
29	21890.0
30	32548.0
31	48952.0
32	74291.0
33	126160.0
34	268617.0
35	682012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10312037699554	18.489835430784122	11.734032951255898	26.67301124096444
2	16.3921892596475	20.700098588969727	36.247228402975594	26.66048374840718
3	18.577494030288104	24.108673695236078	28.77151746023829	28.54231481423753
4	12.684399575731927	15.921197115538233	35.984930846018905	35.40947246271094
5	14.825967888499743	36.2018900303471	33.16803750757898	15.804104573574177
6	34.396136997553675	35.431793863056996	16.518840008198595	13.653229131190736
7	30.054225759339875	30.45012523828005	20.921962601527174	18.5736864008529
8	28.125306861062665	32.741666238035336	19.6038823087486	19.529144592153397
9	27.56255289700223	14.00681445874774	18.64608612839924	39.784546515850785
10	16.205615226231615	26.508041902998393	31.18628005679755	26.100062813972446
11	37.76522537435101	20.901310364928918	22.227339820473617	19.106124440246454
12	24.77270849796673	23.636523753189405	28.359495430790073	23.231272318053794
13	29.515864814355876	19.341482004498292	25.27568787534466	25.866965305801177
14	23.614702522066718	19.822796016690127	24.667109325926585	31.89539213531657
15	25.385573360641047	27.295242815749315	21.891526660089124	25.42765716352051
16	25.8798242455699	25.67345774552392	23.725289404077763	24.721428604828414
17	24.148387488953002	25.780927308803154	25.125900710281073	24.944784491962775
18	25.15754149540897	24.6087375326734	26.13840583437374	24.095315137543896
19	25.84444267796383	25.096208249360558	24.902233091273533	24.15711598140208
20	25.827375357907158	24.36176795614556	24.70740067053526	25.103456015412025
21	26.965118762050384	24.32700985228585	23.872193049314426	24.83567833634934
22	26.01098543121091	24.590501218092296	24.376107622311896	25.0224057283849
23	24.526985848931616	24.267469064508234	25.16814037909713	26.03740470746302
24	24.92421018833281	25.248021671599822	24.713245643157407	25.11452249690996
25	25.0157814260798	24.581071328928562	24.989128350922805	25.414018894068835
26	24.56330261215723	25.41994179965928	25.40708285989055	24.609672728292942
27	25.461869736602154	24.95164259317276	24.519036686165496	25.06745098405959
28	24.44071405302871	24.7914124103576	25.372558554935733	25.39531498167796
29	24.48373305152772	24.951019129426395	25.26041301355878	25.304834805487104
30	24.570082780398923	25.161360210855438	25.394847383868186	24.87370962487745
31	25.1386817170815	24.85196632672306	24.398552317180947	25.61079963901449
32	24.725559052148068	25.098234506536237	24.44811768501676	25.728088756298934
33	24.481083330605678	24.615361834978497	25.213029768835227	25.690525065580594
34	25.15169652278682	24.78533363883057	25.464831189397373	24.598138648985234
35	25.751234847882635	24.569069651811084	25.202430885147066	24.477264615159207
36	24.41515203942785	25.242800162724038	24.899037839573428	25.443009958274686
37	25.5491546610929	25.040330311092823	24.509684729970058	24.900830297844216
38	24.67583781837566	24.723065197162615	25.182947643073238	25.418149341388485
39	25.329461623468426	24.521686407087536	24.552391996595887	25.59645997284815
40	25.582665837459885	24.736547600677707	25.186298760709942	24.494487801152474
41	24.37088611343611	24.9692554440075	25.801813344306296	24.85804509825009
42	25.71600914621316	25.23025295482849	25.097065512011806	23.95667238694654
43	24.67653921509032	24.201148108488926	25.49982309216197	25.62248958425879
44	24.31391711361224	24.82336492735868	25.152943450279547	25.709774508749533
45	24.393486674241753	25.115379759561208	25.26454346087843	25.226590105318614
46	24.193373666273622	24.639618190504173	25.56133212485183	25.60567601837037
47	24.565017137459726	24.394266003924706	25.879200781823535	25.16151607679203
48	25.048591205732123	25.25152865517311	24.849082806896135	24.850797332198628
49	24.109576870741936	25.107586462731675	25.43802224830379	25.3448144182226
50	23.982642755774254	25.507947999965708	24.956727685630632	25.55268155862941
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	17.5
2	21.0
3	51.0
4	81.0
5	92.5
6	104.0
7	113.5
8	123.0
9	187.0
10	251.0
11	343.0
12	435.0
13	731.0
14	1027.0
15	1341.5
16	1656.0
17	2129.5
18	2603.0
19	2990.5
20	3378.0
21	4315.0
22	5252.0
23	5910.0
24	6568.0
25	7891.5
26	9215.0
27	11806.0
28	14397.0
29	16723.5
30	19050.0
31	20754.0
32	22458.0
33	25906.5
34	29355.0
35	34084.5
36	38814.0
37	45338.0
38	51862.0
39	54389.5
40	56917.0
41	61410.5
42	65904.0
43	66364.0
44	66824.0
45	73282.5
46	79741.0
47	85176.0
48	90611.0
49	93058.0
50	95505.0
51	91022.5
52	86540.0
53	85368.5
54	84197.0
55	85792.5
56	87388.0
57	86714.5
58	86041.0
59	78459.0
60	70877.0
61	62968.0
62	55059.0
63	48433.5
64	41808.0
65	36108.5
66	30409.0
67	26747.0
68	23085.0
69	21353.0
70	19621.0
71	15540.0
72	11459.0
73	10368.0
74	9277.0
75	6935.5
76	4594.0
77	4141.5
78	3689.0
79	3242.5
80	2796.0
81	2211.5
82	1627.0
83	1401.5
84	1176.0
85	969.5
86	763.0
87	581.5
88	400.0
89	259.5
90	119.0
91	77.0
92	35.0
93	24.0
94	13.0
95	8.5
96	4.0
97	3.5
98	3.0
99	5.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013092738673612053
2	0.003818715446470182
3	7.793296829530984E-4
4	2.337989048859295E-4
5	0.0
6	5.455307780671689E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.793296829530984E-5
47	0.0
48	0.0
49	0.0
50	7.793296829530984E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1283154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.41269479465962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.00773253970189	47.08284919103981
2	11.22099061519759	12.884546189681584
3	2.960198398110792	5.098589014871257
4	1.214257074353948	2.7885508324855817
5	0.6438131299920249	1.8481523368513324
6	0.4064769569550911	1.4002162482434786
7	0.26488431334735213	1.064540556667316
8	0.19317701533371404	0.8872650418158246
9	0.148207247649574	0.7658079728095374
>10	0.7621942453254901	8.153346292528607
>50	0.08457759304704128	3.450004446610549
>100	0.08286072491752138	9.872242653450623
>500	0.008585887208846028	3.2318797091341236
>1k	0.0020442588592490545	1.472009513810441
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1596	0.12438101739931451	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1504	0.117211184316146	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1451	0.11308073699649458	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1442	0.1123793402818368	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1392	0.1084826918670713	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1328	0.10349498189617147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.793296829530984E-5
2	0.0	0.0	0.0	0.0	7.793296829530984E-5
3	0.0	0.0	0.0	0.0	7.793296829530984E-5
4	0.0	0.0	0.0	0.0	7.793296829530984E-5
5	0.0	0.0	0.0	0.0	7.793296829530984E-5
6	0.0	0.0	0.0	0.0	7.793296829530984E-5
7	0.0	0.0	0.0	7.793296829530984E-5	7.793296829530984E-5
8	0.0	0.0	0.0	7.793296829530984E-5	1.558659365906197E-4
9	0.0	0.0	0.0	2.3379890488592952E-4	1.558659365906197E-4
10	0.0	0.0	0.0	2.3379890488592952E-4	1.558659365906197E-4
11	0.0	0.0	0.0	2.3379890488592952E-4	1.558659365906197E-4
12	0.0	0.0	0.0	3.896648414765492E-4	3.896648414765492E-4
13	0.0	0.0	0.0	3.896648414765492E-4	3.896648414765492E-4
14	0.0	0.0	0.0	3.896648414765492E-4	3.896648414765492E-4
15	0.0	0.0	0.0	3.896648414765492E-4	3.896648414765492E-4
16	0.0	0.0	0.0	3.896648414765492E-4	4.6759780977185904E-4
17	0.0	0.0	0.0	3.896648414765492E-4	4.6759780977185904E-4
18	0.0	0.0	0.0	3.896648414765492E-4	5.455307780671689E-4
19	0.0	0.0	0.0	4.6759780977185904E-4	5.455307780671689E-4
20	0.0	0.0	0.0	4.6759780977185904E-4	6.234637463624788E-4
21	0.0	0.0	0.0	6.234637463624788E-4	7.013967146577885E-4
22	0.0	0.0	0.0	7.013967146577885E-4	7.793296829530984E-4
23	0.0	0.0	0.0	0.0010910615561343377	7.793296829530984E-4
24	0.0	0.0	0.0	0.002026257175678056	7.793296829530984E-4
25	0.0	0.0	0.0	0.0025717879537452246	7.793296829530984E-4
26	0.0	0.0	0.0	0.003351117636698323	8.572626512484082E-4
27	0.0	0.0	0.0	0.0047539110660139005	0.001013128587839028
28	0.0	0.0	0.0	0.010598883688162138	0.001013128587839028
29	0.0	0.0	0.0	0.02275642674223047	0.001013128587839028
30	0.0	0.0	0.0	0.04800670846991086	0.001013128587839028
31	0.0	0.0	0.0	0.10279358518151367	0.001013128587839028
32	0.0	0.0	0.0	0.1757388435059237	0.001013128587839028
33	0.0	0.0	0.0	0.24759304027419934	0.001013128587839028
34	0.0	0.0	0.0	0.32989025479404654	0.0010910615561343377
35	0.0	0.0	0.0	0.42434501236796207	0.0010910615561343377
36	0.0	0.0	0.0	0.5570648573748748	0.0010910615561343377
37	0.0	0.0	0.0	0.7459743725227058	0.0010910615561343377
38	0.0	0.0	0.0	0.9983992568312143	0.0012469274927249575
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	90	1.0017546E-5	19.556196	2
CGCAATA	450	0.0	18.577663	36
TACCGTC	345	0.0	18.49264	7
TAGGACG	335	0.0	18.387945	4
ATAACCG	60	0.007412609	18.333221	4
ACCGTCG	360	0.0	18.33322	8
ATACCGT	360	0.0	18.33322	6
CGTCGTA	365	0.0	17.479345	10
ACACGCT	440	0.0	16.999895	9
TATACCA	105	3.7748356E-5	16.7618	5
CTAGCGG	500	0.0	16.719896	29
TAACGCC	620	0.0	16.677317	4
CAATACG	490	0.0	16.61214	38
ATCGTTT	610	0.0	16.590061	29
GTCTATA	120	6.3956704E-6	16.503115	1
TTAGGAC	375	0.0	16.427204	3
TCTAGCG	510	0.0	16.392056	28
AATAACG	645	0.0	16.37263	2
CCGTCGT	390	0.0	16.358873	9
CTTACAC	135	1.0852E-6	16.296831	3
>>END_MODULE