FastQCFastQC Report
Thu 2 Feb 2017
SRR4062423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794822
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68070.24355755035562193No Hit
GTACATGGAAGCAGTGGTATCAACG52330.18723911576479646No Hit
TCCATGTACTCTGCGTTGATACCAC46610.16677269607867692No Hit
TATCAACGCAGAGTACTTTTTTTTT43900.15707619304556783No Hit
GTACTTTTTTTTTTTTTTTTTTTTT42210.15102929632012344No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31370.11224328418768707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG602.5682435E-514.2508613
AAGACGG6650.013.1427095
CAAGACG7200.012.0055184
CGCAAGA7100.011.9019192
CGCTTAC802.862829E-511.87720718
ACGGTAT4250.011.6250659
CGTCTTA2300.011.5663315
ATCGCCA4700.011.52279816
GCGCAAG7650.011.4163221
AGGCCCG5500.011.401310
GACGGAC7600.011.3762997
CGGTCCA5700.011.33462610
CGAGCCG5600.011.19750615
CGAACGA2050.011.12341916
CGTTATT2400.011.0772542
AGAGCGA7300.011.06270815
CGCATCG4650.011.03292513
GTATTAG6100.010.8935161
CGGACCA7650.010.8053479
CGCCAGT5200.010.780848518
Produced by FastQC (version 0.11.5)