##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062423_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2794822
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202261539375318	32.0	32.0	32.0	32.0	32.0
2	30.889430167645738	32.0	32.0	32.0	32.0	32.0
3	30.876025020555872	32.0	32.0	32.0	32.0	32.0
4	30.939102382906675	32.0	32.0	32.0	32.0	32.0
5	30.75762177340811	32.0	32.0	32.0	32.0	32.0
6	34.41116500442604	36.0	36.0	36.0	32.0	36.0
7	34.29355500994339	36.0	36.0	36.0	32.0	36.0
8	34.2926007452353	36.0	36.0	36.0	32.0	36.0
9	34.43652941045977	36.0	36.0	36.0	32.0	36.0
10	34.125829480374776	36.0	36.0	36.0	32.0	36.0
11	34.43166362652076	36.0	36.0	36.0	32.0	36.0
12	34.21528490902104	36.0	36.0	36.0	32.0	36.0
13	34.33155134745612	36.0	36.0	36.0	32.0	36.0
14	34.24031226317812	36.0	36.0	36.0	32.0	36.0
15	34.18888143860325	36.0	36.0	36.0	32.0	36.0
16	34.19053449557789	36.0	36.0	36.0	32.0	36.0
17	34.12171615938332	36.0	36.0	36.0	32.0	36.0
18	34.13170641994374	36.0	36.0	36.0	32.0	36.0
19	34.11823400560036	36.0	36.0	36.0	32.0	36.0
20	34.10144724780326	36.0	36.0	36.0	32.0	36.0
21	34.079367487446426	36.0	36.0	36.0	32.0	36.0
22	34.03991416984695	36.0	36.0	36.0	32.0	36.0
23	34.0030438432215	36.0	36.0	36.0	32.0	36.0
24	33.97800539712368	36.0	36.0	36.0	32.0	36.0
25	33.533040744634185	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	18.0
4	111.0
5	301.0
6	486.0
7	103.0
8	412.0
9	516.0
10	320.0
11	97.0
12	215.0
13	156.0
14	579.0
15	662.0
16	985.0
17	1322.0
18	1778.0
19	2446.0
20	3622.0
21	5117.0
22	7816.0
23	11139.0
24	15547.0
25	22063.0
26	30598.0
27	39912.0
28	54409.0
29	73060.0
30	95259.0
31	130651.0
32	189694.0
33	275789.0
34	629217.0
35	1200422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06467347294421	17.542690718487986	11.383332790615539	26.009303017952266
2	16.47634992612874	20.117170657455986	37.40880594061603	25.99767347579925
3	19.016941789903484	24.14943068758684	28.453991448774048	28.379636073735625
4	12.642531770051072	15.457542056780458	35.96146833444449	35.93845783872398
5	14.551263557805951	36.50819785967246	33.6745439329825	15.265994649539083
6	33.88462502237336	35.368641489171296	17.076534812958656	13.670198675496689
7	29.779218258909935	30.584404563868162	21.191208832128964	18.445168345092938
8	27.83280975362387	33.103631537404794	19.877084112256636	19.186474596714696
9	27.43501720040758	14.364569846548367	18.93015434702024	39.27025860602381
10	15.932247227478252	26.756921542596064	31.744988898449648	25.56584233147603
11	36.89725052561742	21.272701675348443	22.72920555734075	19.100842241693382
12	24.687229385658643	23.752151031330623	29.047978523185996	22.512641059824738
13	29.46028302380217	19.733677339109114	25.37247987229466	25.43355976479405
14	23.354486792761058	20.067866851428057	25.250237519650003	31.32740883616088
15	24.844008303318283	27.696796344820545	22.586458570274623	24.87273678158655
16	25.250032239113924	25.82528549526443	24.59016921952687	24.33451304609477
17	23.621315791453494	26.010527564011394	25.8449081247387	24.52324851979641
18	24.436188278792482	24.79703924505048	27.240378621228295	23.52639385492874
19	25.402680728470816	25.212612773354802	25.55086566011264	23.833840838061743
20	25.301669243046014	24.64314309921419	25.71705443713426	24.338133220605542
21	26.214560611412974	24.423701281252978	24.607351601870754	24.754386505463295
22	25.28868219587675	25.05364414687531	25.093133692537695	24.56453996471025
23	23.908365984266638	24.717285018909774	25.89225281586307	25.482096180960518
24	24.451955846903065	25.4137203083035	25.64034029185071	24.493983552942726
25	24.549553906995108	24.98278470091956	25.742622500915402	24.72503889116993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	495.0
1	495.0
2	1114.5
3	1734.0
4	1734.0
5	1734.0
6	4578.5
7	7423.0
8	7423.0
9	7423.0
10	7683.0
11	7943.0
12	7943.0
13	7943.0
14	8494.5
15	9046.0
16	9046.0
17	9046.0
18	14766.0
19	20486.0
20	20486.0
21	20486.0
22	32719.0
23	44952.0
24	44952.0
25	44952.0
26	67617.5
27	90283.0
28	90283.0
29	90283.0
30	112936.0
31	135589.0
32	135589.0
33	135589.0
34	161095.5
35	186602.0
36	186602.0
37	186602.0
38	207959.5
39	229317.0
40	229317.0
41	229317.0
42	258708.5
43	288100.0
44	288100.0
45	288100.0
46	330560.5
47	373021.0
48	373021.0
49	373021.0
50	368326.0
51	363631.0
52	363631.0
53	363631.0
54	335682.5
55	307734.0
56	307734.0
57	307734.0
58	281843.0
59	255952.0
60	255952.0
61	255952.0
62	226964.5
63	197977.0
64	197977.0
65	197977.0
66	163971.5
67	129966.0
68	129966.0
69	129966.0
70	98551.5
71	67137.0
72	67137.0
73	67137.0
74	52155.0
75	37173.0
76	37173.0
77	37173.0
78	30273.5
79	23374.0
80	23374.0
81	23374.0
82	16409.5
83	9445.0
84	9445.0
85	9445.0
86	6959.0
87	4473.0
88	4473.0
89	4473.0
90	2994.5
91	1516.0
92	1516.0
93	1516.0
94	902.0
95	288.0
96	288.0
97	288.0
98	726.5
99	1165.0
100	1165.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00876621122919456
2	0.0033991431296876867
3	0.004830361289556186
4	0.015922302028537057
5	0.03735479397256784
6	0.04730176018365392
7	0.06898471530566168
8	0.0896658177157615
9	0.09793110258900209
10	0.11879110726908547
11	0.11961405771100987
12	0.13306750841377374
13	0.12151042177283562
14	0.12430129718457919
15	0.11331669780758845
16	0.11385340461753915
17	0.1246233212705496
18	0.1303124134560269
19	0.1329243865977869
20	0.12297742038670084
21	0.13052709618000718
22	0.15063571132615958
23	0.14086764738505708
24	0.13618040791148775
25	0.13238767978783622
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2794822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.096781833964606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90668604921592	34.52957164715987
2	14.1893951374937	13.081709040179307
3	4.7723007025048645	6.599631129883282
4	1.9826590128871846	3.6557679987281664
5	1.076281353122571	2.480655336342769
6	0.6267152947919405	1.733373492961974
7	0.4292608215285946	1.3851279707910427
8	0.3004897533920936	1.1081288484365732
9	0.22446502201118831	0.9312403634105231
>10	1.257901342937221	10.665653094506569
>50	0.11300568433111532	3.6214837725976805
>100	0.09787530147080739	9.568856984380659
>500	0.016659010857449922	5.333108012341433
>1k	0.00614982176512884	4.873643778357763
>5k	1.5569169025642635E-4	0.432048529922523
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6807	0.24355755035562193	No Hit
GTACATGGAAGCAGTGGTATCAACG	5233	0.18723911576479646	No Hit
TCCATGTACTCTGCGTTGATACCAC	4661	0.16677269607867692	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4390	0.15707619304556783	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4221	0.15102929632012344	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3137	0.11224328418768707	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.156090799342498E-5
2	0.0	0.0	0.0	0.0	7.156090799342498E-5
3	0.0	0.0	0.0	7.156090799342498E-5	7.156090799342498E-5
4	0.0	0.0	0.0	1.0734136199013748E-4	7.156090799342498E-5
5	0.0	0.0	0.0	1.4312181598684996E-4	7.156090799342498E-5
6	0.0	0.0	0.0	1.4312181598684996E-4	2.1468272398027496E-4
7	0.0	0.0	0.0	1.4312181598684996E-4	2.1468272398027496E-4
8	0.0	0.0	0.0	1.4312181598684996E-4	2.1468272398027496E-4
9	0.0	0.0	0.0	1.7890226998356246E-4	2.1468272398027496E-4
10	0.0	0.0	0.0	2.1468272398027496E-4	2.1468272398027496E-4
11	0.0	0.0	0.0	2.1468272398027496E-4	2.1468272398027496E-4
12	0.0	0.0	0.0	2.1468272398027496E-4	3.2202408597041245E-4
13	0.0	0.0	0.0	2.5046317797698745E-4	4.651459019572624E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	60	2.5682435E-5	14.25086	13
AAGACGG	665	0.0	13.142709	5
CAAGACG	720	0.0	12.005518	4
CGCAAGA	710	0.0	11.901919	2
CGCTTAC	80	2.862829E-5	11.877207	18
ACGGTAT	425	0.0	11.625065	9
CGTCTTA	230	0.0	11.56633	15
ATCGCCA	470	0.0	11.522798	16
GCGCAAG	765	0.0	11.416322	1
AGGCCCG	550	0.0	11.4013	10
GACGGAC	760	0.0	11.376299	7
CGGTCCA	570	0.0	11.334626	10
CGAGCCG	560	0.0	11.197506	15
CGAACGA	205	0.0	11.123419	16
CGTTATT	240	0.0	11.077254	2
AGAGCGA	730	0.0	11.062708	15
CGCATCG	465	0.0	11.032925	13
GTATTAG	610	0.0	10.893516	1
CGGACCA	765	0.0	10.805347	9
CGCCAGT	520	0.0	10.7808485	18
>>END_MODULE