FastQCFastQC Report
Thu 2 Feb 2017
SRR4062423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794822
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG12850.019.517734
TGTAGGA17900.019.1737462
AGGACGT14200.018.1269685
TTAGGAC10800.017.7224253
GGACGTG14650.017.5701666
GTAGGAC19050.017.5540073
CTGTAGG18900.017.4633271
ATACCGT6850.017.022096
AGCGAAA9150.016.83049217
GTCCTAA9950.016.806921
GAGCGAA9450.016.52899216
TAGGACA8700.016.4369684
GACGTGA8450.016.402267
GCGAAAG9300.016.32247518
ACCGTCG7050.016.2268458
CACGCGA1105.625793E-515.99989510
TACCGTC6900.015.6230877
CGCAATA8350.015.54480936
CGCACGA850.002981378515.5293128
GTACTAA2001.2751116E-915.4026551
Produced by FastQC (version 0.11.5)