##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2794822
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310441953011676	32.0	32.0	32.0	32.0	32.0
2	31.45568698113869	32.0	32.0	32.0	32.0	32.0
3	31.52938398223572	32.0	32.0	32.0	32.0	32.0
4	31.621261389813018	32.0	32.0	32.0	32.0	32.0
5	31.565886127989547	32.0	32.0	32.0	32.0	32.0
6	35.166894707426806	36.0	36.0	36.0	36.0	36.0
7	35.17857308980679	36.0	36.0	36.0	36.0	36.0
8	35.124098064205874	36.0	36.0	36.0	36.0	36.0
9	35.237430147608684	36.0	36.0	36.0	36.0	36.0
10	35.1295145093319	36.0	36.0	36.0	36.0	36.0
11	35.25742533871567	36.0	36.0	36.0	36.0	36.0
12	35.16456039060806	36.0	36.0	36.0	36.0	36.0
13	35.209521035686706	36.0	36.0	36.0	36.0	36.0
14	35.17343179637201	36.0	36.0	36.0	36.0	36.0
15	35.1316488134128	36.0	36.0	36.0	36.0	36.0
16	35.145913407007676	36.0	36.0	36.0	36.0	36.0
17	35.12940323211997	36.0	36.0	36.0	36.0	36.0
18	35.14027726989411	36.0	36.0	36.0	36.0	36.0
19	35.11861005817186	36.0	36.0	36.0	36.0	36.0
20	35.10730307690436	36.0	36.0	36.0	36.0	36.0
21	35.100046443029285	36.0	36.0	36.0	36.0	36.0
22	35.081556177817404	36.0	36.0	36.0	36.0	36.0
23	35.04620008000509	36.0	36.0	36.0	36.0	36.0
24	35.022541328213386	36.0	36.0	36.0	36.0	36.0
25	34.997634911990815	36.0	36.0	36.0	36.0	36.0
26	34.96080823751924	36.0	36.0	36.0	36.0	36.0
27	34.94428553947264	36.0	36.0	36.0	36.0	36.0
28	34.929292098029855	36.0	36.0	36.0	32.0	36.0
29	34.9046543930168	36.0	36.0	36.0	32.0	36.0
30	34.891839265613335	36.0	36.0	36.0	32.0	36.0
31	34.884020520805976	36.0	36.0	36.0	32.0	36.0
32	34.842370641135645	36.0	36.0	36.0	32.0	36.0
33	34.83196890535426	36.0	36.0	36.0	32.0	36.0
34	34.80907621308262	36.0	36.0	36.0	32.0	36.0
35	34.7818533702683	36.0	36.0	36.0	32.0	36.0
36	34.751327991550085	36.0	36.0	36.0	32.0	36.0
37	34.73512087710774	36.0	36.0	36.0	32.0	36.0
38	34.69569332143514	36.0	36.0	36.0	32.0	36.0
39	34.668597141427966	36.0	36.0	36.0	32.0	36.0
40	34.658083412825576	36.0	36.0	36.0	32.0	36.0
41	34.63203989377499	36.0	36.0	36.0	32.0	36.0
42	34.59392512295953	36.0	36.0	36.0	32.0	36.0
43	34.59128667228181	36.0	36.0	36.0	32.0	36.0
44	34.55004898344152	36.0	36.0	36.0	32.0	36.0
45	34.502103890695004	36.0	36.0	36.0	32.0	36.0
46	34.504193827012955	36.0	36.0	36.0	32.0	36.0
47	34.468998383439086	36.0	36.0	36.0	32.0	36.0
48	34.43242968604083	36.0	36.0	36.0	32.0	36.0
49	34.421358497965166	36.0	36.0	36.0	32.0	36.0
50	33.91054170891742	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	19.0
21	63.0
22	210.0
23	659.0
24	1750.0
25	3951.0
26	8468.0
27	15833.0
28	26999.0
29	41922.0
30	60169.0
31	87013.0
32	128711.0
33	209822.0
34	464917.0
35	1744310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5207292861018	18.209034914063967	11.848644648375824	26.42159115145841
2	15.906414585561501	20.625904163904003	37.16991643852723	26.297764812007262
3	18.494560097265968	24.50226509055532	28.7104924089679	28.292682403210804
4	12.395668407390113	15.938693616572117	36.1090090041738	35.556628971863965
5	14.21933640830665	36.845758637136335	33.662298945084494	15.27260600947252
6	34.14902676399027	35.4842206955775	16.8466795477315	13.52007299270073
7	29.800012236919642	30.600385498749294	21.12900969328626	18.4705925710448
8	27.90431734114015	33.243762930161566	19.7300937233212	19.121826005377088
9	27.455523106659385	14.232212284002344	18.837586078827204	39.47467853051106
10	15.954432876226107	26.840385541547906	31.756405237972224	25.448776344253766
11	37.185516644709395	21.079589326261207	22.69089051109516	19.04400351793424
12	24.682072776012213	23.751709411189694	29.039845829179818	22.526371983618276
13	29.662890874624576	19.67967906363983	25.360219720611905	25.297210341123694
14	23.3804872009738	20.01558596576097	25.211408812439572	31.392518020825655
15	24.969783406599777	27.67095722017359	22.53789328980522	24.821366083421413
16	25.409704088489356	25.74707798922436	24.5472162448986	24.296001677387682
17	23.74931212077191	26.008060620676382	25.771265576126133	24.47136168242557
18	24.587361914282912	24.76544123382455	27.205059928682402	23.442136923210136
19	25.524845589450777	25.17773940522867	25.53228792388209	23.76512708143846
20	25.495899202167436	24.533118746023895	25.661562704172212	24.309419347636453
21	26.352805294934704	24.364950612239348	24.55934581880349	24.72289827402246
22	25.39986446364026	25.024169696674782	25.046496699968728	24.52946913971623
23	24.025072079724577	24.633482919484674	25.853131254870615	25.48831374592013
24	24.633912284932634	25.2655446393366	25.60449288004746	24.496050195683303
25	24.758392484387198	24.729267194833877	25.76003051357117	24.75230980720776
26	23.99891656785298	25.481479679206764	26.25945409045728	24.260149662482977
27	24.931033174921335	25.270410781080155	25.315601494478003	24.482954549520507
28	24.016484770765363	25.05855471296562	25.93116842503744	24.99379209123157
29	24.1532376659408	25.23387893754951	25.749260596918155	24.863622799591532
30	24.314070806656023	25.1936974877112	26.115437763120514	24.376793942512258
31	24.76994957102814	24.912999826106994	25.209190424291776	25.107860178573087
32	24.28558956527464	25.334350452372277	25.31878595488371	25.06127402746937
33	23.95050561359543	24.886558070603424	26.01521671147572	25.14771960432543
34	24.558308185637586	25.08610566254309	26.261815600421063	24.093770551398265
35	25.276135653719628	25.014258510917692	25.737560388461233	23.97204544690145
36	24.15398905547473	25.40651962808365	25.679846516164535	24.759644800277083
37	25.05483354574996	25.206363768426037	25.15423164695283	24.584571038871168
38	24.32988576732257	25.253236163161734	25.565349063375052	24.85152900614064
39	24.79506744973383	24.724758857630288	25.325190656149122	25.15498303648676
40	25.33206050331649	24.91654209105267	25.765469142578667	23.985928263052173
41	24.239826364612846	24.930031322209427	26.351875003130793	24.478267310046935
42	25.291091883490253	25.578874074985812	25.59826708105203	23.5317669604719
43	24.235604271041232	24.737782942885094	25.919539777488517	25.10707300858516
44	23.915190305500673	25.05179220716024	25.901363306858183	25.131654180480904
45	23.957196558492814	25.453749827359314	25.814953510456125	24.774100103691758
46	23.9535454544804	24.733632028990797	26.021014606317834	25.291807910210967
47	24.50792930641021	24.654557606888737	26.183277503898278	24.65423558280277
48	24.512258741343814	25.694122917309226	25.25967664488114	24.53394169646582
49	23.862235233585537	25.286225741746705	25.877640865858364	24.973898158809398
50	23.538994612179238	26.145135539937787	25.28329174451897	25.032578103364006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	80.5
2	96.0
3	317.0
4	538.0
5	599.0
6	660.0
7	578.5
8	497.0
9	681.0
10	865.0
11	1316.5
12	1768.0
13	3005.0
14	4242.0
15	5459.0
16	6676.0
17	7791.0
18	8906.0
19	9556.5
20	10207.0
21	11884.5
22	13562.0
23	14541.0
24	15520.0
25	18398.5
26	21277.0
27	26801.0
28	32325.0
29	37519.5
30	42714.0
31	47442.0
32	52170.0
33	61541.5
34	70913.0
35	81879.5
36	92846.0
37	106217.0
38	119588.0
39	124340.0
40	129092.0
41	138112.5
42	147133.0
43	147506.0
44	147879.0
45	163274.5
46	178670.0
47	190460.0
48	202250.0
49	205219.0
50	208188.0
51	199320.0
52	190452.0
53	187130.0
54	183808.0
55	184902.0
56	185996.0
57	182325.5
58	178655.0
59	163452.5
60	148250.0
61	130227.5
62	112205.0
63	98043.5
64	83882.0
65	71189.5
66	58497.0
67	52102.5
68	45708.0
69	41381.0
70	37054.0
71	29146.5
72	21239.0
73	18744.5
74	16250.0
75	11905.0
76	7560.0
77	6771.5
78	5983.0
79	5172.5
80	4362.0
81	3450.5
82	2539.0
83	2185.5
84	1832.0
85	1469.0
86	1106.0
87	834.5
88	563.0
89	349.0
90	135.0
91	89.5
92	44.0
93	35.5
94	27.0
95	21.5
96	16.0
97	12.5
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014956229770625822
2	0.004043191301628511
3	3.9358499396383744E-4
4	2.5046317797698745E-4
5	3.578045399671249E-5
6	7.871699879276749E-4
7	3.578045399671249E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.4312181598684996E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2794822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.92228628904065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.71408558335175	37.7982025660501
2	14.084274003704245	14.062383179724321
3	4.50841478960597	6.752111215093626
4	1.9250314070078465	3.844078760641618
5	0.9648574769106047	2.4083945595226317
6	0.5710732288926412	1.7105568734871162
7	0.39852732843389405	1.392677676885837
8	0.2780862100401427	1.1106159512526637
9	0.21449099337981603	0.9637092700135109
>10	1.1493927374473163	10.38621381390556
>50	0.09304353638078773	3.224143239828123
>100	0.08248068614378055	8.810941908677188
>500	0.011694253464902219	4.024174486761179
>1k	0.004547765236339784	3.511796498156679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.156090799342498E-5
2	0.0	0.0	0.0	0.0	7.156090799342498E-5
3	0.0	0.0	0.0	7.156090799342498E-5	7.156090799342498E-5
4	0.0	0.0	0.0	1.0734136199013748E-4	7.156090799342498E-5
5	0.0	0.0	0.0	1.4312181598684996E-4	7.156090799342498E-5
6	0.0	0.0	0.0	1.4312181598684996E-4	1.7890226998356246E-4
7	0.0	0.0	0.0	1.4312181598684996E-4	2.1468272398027496E-4
8	0.0	0.0	0.0	1.4312181598684996E-4	2.1468272398027496E-4
9	0.0	0.0	0.0	2.5046317797698745E-4	2.1468272398027496E-4
10	0.0	3.578045399671249E-5	0.0	2.862436319736999E-4	2.1468272398027496E-4
11	0.0	3.578045399671249E-5	0.0	2.862436319736999E-4	2.1468272398027496E-4
12	0.0	3.578045399671249E-5	0.0	2.862436319736999E-4	6.798286259375373E-4
13	0.0	3.578045399671249E-5	0.0	3.2202408597041245E-4	8.587308959210998E-4
14	0.0	3.578045399671249E-5	0.0	3.578045399671249E-4	8.587308959210998E-4
15	0.0	3.578045399671249E-5	0.0	4.293654479605499E-4	0.0010376331659046622
16	0.0	3.578045399671249E-5	0.0	4.651459019572624E-4	0.0011091940738980873
17	0.0	3.578045399671249E-5	0.0	4.651459019572624E-4	0.0011091940738980873
18	0.0	3.578045399671249E-5	0.0	5.724872639473998E-4	0.0012165354358882247
19	0.0	7.156090799342498E-5	0.0	6.082677179441124E-4	0.0012165354358882247
20	0.0	7.156090799342498E-5	0.0	7.513895339309624E-4	0.0014312181598684997
21	0.0	7.156090799342498E-5	0.0	9.660722579112373E-4	0.0014312181598684997
22	0.0	7.156090799342498E-5	0.0	0.0015743399758553496	0.001466998613865212
23	0.0	7.156090799342498E-5	0.0	0.002540412233766587	0.001466998613865212
24	0.0	7.156090799342498E-5	0.0	0.004472556749589062	0.001466998613865212
25	0.0	7.156090799342498E-5	0.0	0.005545970369490436	0.001466998613865212
26	0.0	7.156090799342498E-5	0.0	0.0072992126153293485	0.0015027790678619247
27	0.0	7.156090799342498E-5	0.0	0.009374478947138673	0.0015027790678619247
28	0.0	7.156090799342498E-5	3.578045399671249E-5	0.016101204298520622	0.0015027790678619247
29	0.0	7.156090799342498E-5	3.578045399671249E-5	0.03270333495299522	0.0015027790678619247
30	0.0	7.156090799342498E-5	3.578045399671249E-5	0.06039740634645069	0.0015027790678619247
31	0.0	7.156090799342498E-5	3.578045399671249E-5	0.1185764245451052	0.0015743399758553496
32	0.0	7.156090799342498E-5	3.578045399671249E-5	0.18745379848877675	0.0015743399758553496
33	0.0	7.156090799342498E-5	1.0734136199013748E-4	0.25439902791662583	0.0015743399758553496
34	0.0	7.156090799342498E-5	1.4312181598684996E-4	0.3327224417154295	0.0017174617918421997
35	0.0	7.156090799342498E-5	1.4312181598684996E-4	0.41852397039954603	0.0017174617918421997
36	0.0	7.156090799342498E-5	1.4312181598684996E-4	0.5441133639280069	0.0017174617918421997
37	0.0	7.156090799342498E-5	1.4312181598684996E-4	0.7239459257154839	0.0017174617918421997
38	0.0	7.156090799342498E-5	1.4312181598684996E-4	0.9712604237407606	0.0018248031538323371
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	1285	0.0	19.51773	4
TGTAGGA	1790	0.0	19.173746	2
AGGACGT	1420	0.0	18.126968	5
TTAGGAC	1080	0.0	17.722425	3
GGACGTG	1465	0.0	17.570166	6
GTAGGAC	1905	0.0	17.554007	3
CTGTAGG	1890	0.0	17.463327	1
ATACCGT	685	0.0	17.02209	6
AGCGAAA	915	0.0	16.830492	17
GTCCTAA	995	0.0	16.80692	1
GAGCGAA	945	0.0	16.528992	16
TAGGACA	870	0.0	16.436968	4
GACGTGA	845	0.0	16.40226	7
GCGAAAG	930	0.0	16.322475	18
ACCGTCG	705	0.0	16.226845	8
CACGCGA	110	5.625793E-5	15.999895	10
TACCGTC	690	0.0	15.623087	7
CGCAATA	835	0.0	15.544809	36
CGCACGA	85	0.0029813785	15.52931	28
GTACTAA	200	1.2751116E-9	15.402655	1
>>END_MODULE