FastQCFastQC Report
Thu 2 Feb 2017
SRR4062422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747623
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG79301.0606950294466595No Hit
TCCATGTACTCTGCGTTGATACCAC68120.9111544187377862No Hit
GTCCTACAGTGGACATTTCTAAATT53740.7188114865380011No Hit
GTCCTAAAGTGTGTATTTCTCATTT49900.6674487007489069No Hit
CTGTAGGACGTGGAATATGGCAAGA48710.651531587444474No Hit
CTTTAGGACGTGAAATATGGCGAGG41580.556162664872536No Hit
GTCCTACAGTGTGCATTTCTCATTT38990.5215195359158292No Hit
GAGTACATGGAAGCAGTGGTATCAA35690.4773796418783264No Hit
CATGTACTCTGCGTTGATACCACTG30850.4126411306233222No Hit
CTGTAGGACCTGGAATATGGCGAGA30660.41009974278479927No Hit
TTGTAGAACAGTGTATATCAATGAG30130.40301060828786706No Hit
GATATACACTGTTCTACAAATCCCG27320.36542481972866003No Hit
GTGTATATCAATGAGTTACAATGAA25600.3424185719272949No Hit
CTGAAGGACCTGGAATATGGCGAGA23120.3092467727718382No Hit
GTATCAACGCAGAGTACTTTTTTTT22170.29653983357922375No Hit
GCTTCCATGTACTCTGCGTTGATAC22150.29627231906990553No Hit
GTGTATATCAATGAGTTACAATGAG22040.29480098926865544No Hit
GTCCTTCAGTGTGCATTTCTCATTT20490.274068614796495No Hit
CCCATGTACTCTGCGTTGATACCAC19860.26564190775297175No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19740.2640368206970626No Hit
GTTCTACAGTGTGGTTTTTATCATT19410.2596228312933123No Hit
GCGTTGATACCACTGCTTCCATGTA18980.253871269342971No Hit
GTACATGGGAAGCAGTGGTATCAAC18420.24638086308206142No Hit
CATGGAAGCAGTGGTATCAACGCAG18340.2453108050447886No Hit
ATTTAGAAATGTCCACTGTAGGACG18300.24477577602615222No Hit
TTTCTAAATTTTCCACCTTTTTCAG16190.21655299529308222No Hit
GATATACACTGTTCTACAATGCCGG15930.21307530667194563No Hit
TATCAACGCAGAGTACTTTTTTTTT15410.20611992942967242No Hit
GTATCAACGCAGAGTACATGGAAGC15180.20304351257251313No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14270.1908716023985351No Hit
GAATATGGCAAGAAAACTGAAAATC14180.18966778710660318No Hit
CTGTAGAACATATTAGATGAGTGAG14050.1879289427960349No Hit
ACTCTGCGTTGATACCACTGCTTCC13780.18431749692023922No Hit
CTGTAGGACATGGAATATGGCAAGA13210.17669333340467053No Hit
ATGTTCTACAGTGTGGTTTTTATCA12630.16893541263444276No Hit
GTTCTACAAATCCCGTTTCCAACGA12360.16532396675864705No Hit
GGAATATGGCGAGAAAACTGAAAAT12190.16305009342944238No Hit
CAGTGGTATCAACGCAGAGTACATG12160.16264882166546507No Hit
ACGCAGAGTACATGGAAGCAGTGGT11980.1602411910816013No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11280.15087818325546432No Hit
ACCTGGAATATGGCGAGAAAACTGA10910.14592916483307763No Hit
GACCTGGAATATGGCGAGAAAACTG9870.1320184103485313No Hit
GTATCAACGCAGAGTACATGGGAAG9680.1294770225100084No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9660.1292095080006902No Hit
GTCCACTGTAGGACGTGGAATATGG9610.12854072172739467No Hit
GTAGAACATATTAGATGAGTGAGTT9560.1278719354540992No Hit
ACAGTGGACATTTCTAAATTTTCCA9460.1265343629075082No Hit
GTAGAACAGTGTATATCAATGAGTT9410.1258655766342127No Hit
TATCAACGCAGAGTACATGGAAGCA9300.1243942468329626No Hit
TTCCAGGTCCTTCAGTGTGCATTTC9260.1238592178143262No Hit
GAAATATGGCGAGGAAAACTGAAAA9060.12118407272114422No Hit
CATCTAATATGTTCTACAGTGTGGT9020.12064904370250781No Hit
GCCATATTCCACGTCCTACAGTGGA9010.12051528644784872No Hit
GTACATGGGGTGGTATCAACGCAAA8980.12011401468387142No Hit
CCATATTCCAGGTCCTTCAGTGTGC8790.11757262684534853No Hit
CCATATTTCACGTCCTAAAGTGTGT8430.11275736567762093No Hit
CTACAGTGGACATTTCTAAATTTTC8200.10968094882046166No Hit
GGTATCAACGCAGAGTACTTTTTTT7950.10633701745398416No Hit
GTGTATTTCTCATTTTCCGTGATTT7920.10593574569000687No Hit
ATACACACTTTAGGACGTGAAATAT7870.10526695941671137No Hit
CCACTGTAGGACGTGGAATATGGCA7820.10459817314341588No Hit
CACTTTAGGACGTGAAATATGGCGA7790.10419690137943856No Hit
TATTAGATGAGTGAGTTACACTGAA7520.10058545550364287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACG350.002166890516.28783817
GAGGTCG350.002166890516.28783817
TATGACT508.7095126E-515.1989294
TAGAACC456.760122E-414.7767364
TGTGCAA801.283297E-714.25185910
TCGAGGT400.0052711414.25185915
CCCTTGG400.00530402514.2385051
TTGTAGA5000.013.8588121
TAGGACC11600.013.8395424
GCAATTC707.2457096E-613.57228913
TAGAAAT5250.013.3895314
AAATGTC5750.013.218237
TGGCGAA801.9925155E-613.06420418
CTAGGAC1750.013.02590753
TCCAACG4200.012.89453918
CCAACGA2800.012.89453919
AATGTCC6200.012.7185498
AACTTTT1051.983426E-812.66831915
GGCGAAA751.4741689E-512.66831919
TGCAATT751.4741689E-512.66831912
Produced by FastQC (version 0.11.5)