##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062422_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747623
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204770318730162	32.0	32.0	32.0	32.0	32.0
2	31.04968814496076	32.0	32.0	32.0	32.0	32.0
3	31.02653476417927	32.0	32.0	32.0	32.0	32.0
4	31.041733601026184	32.0	32.0	32.0	32.0	32.0
5	30.999699046177017	32.0	32.0	32.0	32.0	32.0
6	34.64895274757465	36.0	36.0	36.0	32.0	36.0
7	34.500145126621305	36.0	36.0	36.0	32.0	36.0
8	34.49902691597236	36.0	36.0	36.0	32.0	36.0
9	34.55823322717466	36.0	36.0	36.0	32.0	36.0
10	34.379375701389606	36.0	36.0	36.0	32.0	36.0
11	34.594617875587026	36.0	36.0	36.0	32.0	36.0
12	34.44051480492173	36.0	36.0	36.0	32.0	36.0
13	34.488315635019255	36.0	36.0	36.0	32.0	36.0
14	34.44952603116811	36.0	36.0	36.0	32.0	36.0
15	34.421012997192435	36.0	36.0	36.0	32.0	36.0
16	34.44062181072546	36.0	36.0	36.0	32.0	36.0
17	34.40108717896587	36.0	36.0	36.0	32.0	36.0
18	34.44052283035701	36.0	36.0	36.0	32.0	36.0
19	34.3433000322355	36.0	36.0	36.0	32.0	36.0
20	34.30903677388202	36.0	36.0	36.0	32.0	36.0
21	34.21663726236352	36.0	36.0	36.0	32.0	36.0
22	34.172065332393466	36.0	36.0	36.0	32.0	36.0
23	34.13569539727911	36.0	36.0	36.0	32.0	36.0
24	34.093648804277024	36.0	36.0	36.0	32.0	36.0
25	33.64506576175425	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	22.0
5	79.0
6	117.0
7	21.0
8	105.0
9	151.0
10	76.0
11	32.0
12	73.0
13	43.0
14	176.0
15	181.0
16	280.0
17	390.0
18	509.0
19	719.0
20	1015.0
21	1392.0
22	2008.0
23	2783.0
24	3759.0
25	4840.0
26	6913.0
27	8859.0
28	12296.0
29	16289.0
30	21909.0
31	30753.0
32	45272.0
33	66960.0
34	159276.0
35	360321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34163594433059	19.133549861147525	12.505417654329577	25.019396540192307
2	10.669011965034544	21.43767681699316	47.889298350042466	20.00401286792983
3	20.901184332672546	26.62581561649772	29.622228107769725	22.850771943060003
4	10.5714908742597	17.843320610942325	39.80048187226505	31.784706642532917
5	9.88874393711323	40.840809499916375	37.39407927747115	11.876367285499247
6	29.236930916448323	38.64121140428584	19.74112067742721	12.380737001838623
7	24.764196716220937	32.1373742349025	23.361088543179406	19.73734050569716
8	21.913417861833253	43.846916932073306	21.350474652829377	12.889190553264065
9	26.23395715123456	15.621376719426541	20.485576498247436	37.65908963109146
10	14.151719113672826	30.932127351276645	36.651391012987425	18.264762522063098
11	33.907323259716385	20.6530575074695	27.865295840514904	17.574323392299217
12	23.9552485692052	23.87515352665973	35.9929441357169	16.176653768418177
13	32.17151642417879	23.113978229659946	26.1439606591099	18.570544687051363
14	19.212483058247486	25.54160037285248	27.3139889322812	27.93192763661883
15	20.807538670234063	37.72990895371088	24.60767338499631	16.85487899105874
16	18.480527184807947	27.77067300159757	36.0737533796044	17.675046433990083
17	18.078160845651013	30.542620709235813	32.98928204571905	18.389936399394127
18	18.55183923194788	27.011743654033666	39.09881384808255	15.337603265935904
19	23.279075405260247	27.296385438891857	27.238256727996475	22.18628242785142
20	21.865290664038227	29.328221088945554	32.5393998157235	16.26708843129272
21	20.163533721296215	28.23923336034341	26.20356666912213	25.39366624923825
22	20.35591946531701	33.938946575909526	27.915858966509973	17.789274992263493
23	18.759585671288328	32.36872737111835	29.24679562310714	19.62489133448618
24	22.320327139418666	29.556532140001313	30.835810140196866	17.287330580383152
25	21.29822225734342	30.07201779751708	30.68919840186654	17.94056154327296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	130.0
2	356.0
3	582.0
4	582.0
5	582.0
6	1760.0
7	2938.0
8	2938.0
9	2938.0
10	2907.5
11	2877.0
12	2877.0
13	2877.0
14	2856.5
15	2836.0
16	2836.0
17	2836.0
18	5192.0
19	7548.0
20	7548.0
21	7548.0
22	14672.5
23	21797.0
24	21797.0
25	21797.0
26	37022.0
27	52247.0
28	52247.0
29	52247.0
30	74669.0
31	97091.0
32	97091.0
33	97091.0
34	97649.5
35	98208.0
36	98208.0
37	98208.0
38	95687.5
39	93167.0
40	93167.0
41	93167.0
42	95188.5
43	97210.0
44	97210.0
45	97210.0
46	110055.5
47	122901.0
48	122901.0
49	122901.0
50	97974.0
51	73047.0
52	73047.0
53	73047.0
54	52835.0
55	32623.0
56	32623.0
57	32623.0
58	26231.5
59	19840.0
60	19840.0
61	19840.0
62	15460.0
63	11080.0
64	11080.0
65	11080.0
66	8531.0
67	5982.0
68	5982.0
69	5982.0
70	4429.0
71	2876.0
72	2876.0
73	2876.0
74	2174.5
75	1473.0
76	1473.0
77	1473.0
78	996.0
79	519.0
80	519.0
81	519.0
82	360.5
83	202.0
84	202.0
85	202.0
86	132.0
87	62.0
88	62.0
89	62.0
90	50.5
91	39.0
92	39.0
93	39.0
94	37.0
95	35.0
96	35.0
97	35.0
98	174.0
99	313.0
100	313.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008961736062159671
2	0.0037452031304547884
3	0.0038789603851138877
4	0.01631838506841015
5	0.0331717991554567
6	0.04347110776420736
7	0.0663435983109134
8	0.08774475905636932
9	0.0959039515905744
10	0.12051528644784872
11	0.12305667428637161
12	0.13509482720569058
13	0.12292291703171251
14	0.12666812016216727
15	0.11503123900682563
16	0.11556626802546202
17	0.1246617613422808
18	0.13469355544171327
19	0.13429228367773596
20	0.12292291703171251
21	0.13215216760319037
22	0.15542592991387372
23	0.14258523346660015
24	0.13776997229887256
25	0.13402476916841777
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	747623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.89607494691395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94346932328989	33.43468901714331
2	13.075018139718415	11.21733916107241
3	3.8639037287176126	4.97238911804193
4	1.6135732266191596	2.7686383224555695
5	0.8242404884221497	1.7678340882818746
6	0.4917025297345282	1.2655265140247702
7	0.32415974469638825	0.9733626492288134
8	0.24068298531241464	0.8259484301126663
9	0.1790462798693862	0.691234437621899
>10	1.1757934497379567	9.804356329770954
>50	0.12654958791997323	3.852210619765075
>100	0.11311345105351829	9.803835303700467
>500	0.015935872938478743	4.960022495842824
>1k	0.011873787679650829	10.966334293200283
>5k	9.374042904987496E-4	2.696279219737177
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7930	1.0606950294466595	No Hit
TCCATGTACTCTGCGTTGATACCAC	6812	0.9111544187377862	No Hit
GTCCTACAGTGGACATTTCTAAATT	5374	0.7188114865380011	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4990	0.6674487007489069	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4871	0.651531587444474	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4158	0.556162664872536	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3899	0.5215195359158292	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3569	0.4773796418783264	No Hit
CATGTACTCTGCGTTGATACCACTG	3085	0.4126411306233222	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3066	0.41009974278479927	No Hit
TTGTAGAACAGTGTATATCAATGAG	3013	0.40301060828786706	No Hit
GATATACACTGTTCTACAAATCCCG	2732	0.36542481972866003	No Hit
GTGTATATCAATGAGTTACAATGAA	2560	0.3424185719272949	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2312	0.3092467727718382	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2217	0.29653983357922375	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2215	0.29627231906990553	No Hit
GTGTATATCAATGAGTTACAATGAG	2204	0.29480098926865544	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2049	0.274068614796495	No Hit
CCCATGTACTCTGCGTTGATACCAC	1986	0.26564190775297175	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1974	0.2640368206970626	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1941	0.2596228312933123	No Hit
GCGTTGATACCACTGCTTCCATGTA	1898	0.253871269342971	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1842	0.24638086308206142	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1834	0.2453108050447886	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1830	0.24477577602615222	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1619	0.21655299529308222	No Hit
GATATACACTGTTCTACAATGCCGG	1593	0.21307530667194563	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1541	0.20611992942967242	No Hit
GTATCAACGCAGAGTACATGGAAGC	1518	0.20304351257251313	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1427	0.1908716023985351	No Hit
GAATATGGCAAGAAAACTGAAAATC	1418	0.18966778710660318	No Hit
CTGTAGAACATATTAGATGAGTGAG	1405	0.1879289427960349	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1378	0.18431749692023922	No Hit
CTGTAGGACATGGAATATGGCAAGA	1321	0.17669333340467053	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1263	0.16893541263444276	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1236	0.16532396675864705	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1219	0.16305009342944238	No Hit
CAGTGGTATCAACGCAGAGTACATG	1216	0.16264882166546507	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1198	0.1602411910816013	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1128	0.15087818325546432	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1091	0.14592916483307763	No Hit
GACCTGGAATATGGCGAGAAAACTG	987	0.1320184103485313	No Hit
GTATCAACGCAGAGTACATGGGAAG	968	0.1294770225100084	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	966	0.1292095080006902	No Hit
GTCCACTGTAGGACGTGGAATATGG	961	0.12854072172739467	No Hit
GTAGAACATATTAGATGAGTGAGTT	956	0.1278719354540992	No Hit
ACAGTGGACATTTCTAAATTTTCCA	946	0.1265343629075082	No Hit
GTAGAACAGTGTATATCAATGAGTT	941	0.1258655766342127	No Hit
TATCAACGCAGAGTACATGGAAGCA	930	0.1243942468329626	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	926	0.1238592178143262	No Hit
GAAATATGGCGAGGAAAACTGAAAA	906	0.12118407272114422	No Hit
CATCTAATATGTTCTACAGTGTGGT	902	0.12064904370250781	No Hit
GCCATATTCCACGTCCTACAGTGGA	901	0.12051528644784872	No Hit
GTACATGGGGTGGTATCAACGCAAA	898	0.12011401468387142	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	879	0.11757262684534853	No Hit
CCATATTTCACGTCCTAAAGTGTGT	843	0.11275736567762093	No Hit
CTACAGTGGACATTTCTAAATTTTC	820	0.10968094882046166	No Hit
GGTATCAACGCAGAGTACTTTTTTT	795	0.10633701745398416	No Hit
GTGTATTTCTCATTTTCCGTGATTT	792	0.10593574569000687	No Hit
ATACACACTTTAGGACGTGAAATAT	787	0.10526695941671137	No Hit
CCACTGTAGGACGTGGAATATGGCA	782	0.10459817314341588	No Hit
CACTTTAGGACGTGAAATATGGCGA	779	0.10419690137943856	No Hit
TATTAGATGAGTGAGTTACACTGAA	752	0.10058545550364287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTACG	35	0.0021668905	16.287838	17
GAGGTCG	35	0.0021668905	16.287838	17
TATGACT	50	8.7095126E-5	15.198929	4
TAGAACC	45	6.760122E-4	14.776736	4
TGTGCAA	80	1.283297E-7	14.251859	10
TCGAGGT	40	0.00527114	14.251859	15
CCCTTGG	40	0.005304025	14.238505	1
TTGTAGA	500	0.0	13.858812	1
TAGGACC	1160	0.0	13.839542	4
GCAATTC	70	7.2457096E-6	13.572289	13
TAGAAAT	525	0.0	13.389531	4
AAATGTC	575	0.0	13.21823	7
TGGCGAA	80	1.9925155E-6	13.064204	18
CTAGGAC	175	0.0	13.0259075	3
TCCAACG	420	0.0	12.894539	18
CCAACGA	280	0.0	12.894539	19
AATGTCC	620	0.0	12.718549	8
AACTTTT	105	1.983426E-8	12.668319	15
GGCGAAA	75	1.4741689E-5	12.668319	19
TGCAATT	75	1.4741689E-5	12.668319	12
>>END_MODULE