FastQCFastQC Report
Thu 2 Feb 2017
SRR4062422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747623
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA37660.5037298210461689No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33430.4471505023253699No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA27030.36154585934354616No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC20240.27072468343001754No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA19330.2585527732560395No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA18210.24357196073422033No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15200.20331102708183135No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG14590.19515183454762627No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC14500.19394801925569438No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC14450.19327923298239888No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA13700.1832474388829664No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC13660.18271240986433002No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA13430.17963599300717073No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA9540.12760442094478097No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA9210.12319043154103071No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9150.12238788801307611No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG9140.12225413075841701No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG8690.11623505429875752No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA8440.11289112293228004No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8430.11275736567762093No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC8270.11061724960307534No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA7830.10473193039807498No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT7550.10098672726762017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT301.3007413E-436.6662756
TCGTATA401.8192814E-532.9996544
TACGGCG454.067787E-529.33302116
GTCGTAT602.9431067E-729.33302143
TCGTATC300.00574392729.33302144
TAGGACC13050.027.8157964
CGTGTAA407.030044E-427.4997069
ACGATTT609.747284E-625.66639329
CTAGAGT1900.025.4734134
GACGTGT450.001397801924.4441857
TCACGAT551.59294E-423.99974627
ACGGCGA551.59294E-423.99974617
CACGATT651.8123405E-523.69205528
TGTAGGA35800.023.3548322
CTGTAGG34950.023.2979581
GGACCTG23150.022.617476
ATACACT15100.022.582544
AGGACCT24200.022.5452145
ACTGTTC13700.022.4815128
ATAGGAC2650.022.4148563
Produced by FastQC (version 0.11.5)