##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747623
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23185616279863	32.0	32.0	32.0	32.0	32.0
2	31.51831738723929	32.0	32.0	32.0	32.0	32.0
3	31.589458858274824	32.0	32.0	32.0	32.0	32.0
4	31.673814208498133	32.0	32.0	32.0	32.0	32.0
5	31.648098038717375	32.0	32.0	32.0	32.0	32.0
6	35.268005398442796	36.0	36.0	36.0	36.0	36.0
7	35.287537970340665	36.0	36.0	36.0	36.0	36.0
8	35.236127031939894	36.0	36.0	36.0	36.0	36.0
9	35.33437708577719	36.0	36.0	36.0	36.0	36.0
10	35.26272332445631	36.0	36.0	36.0	36.0	36.0
11	35.33936221865834	36.0	36.0	36.0	36.0	36.0
12	35.283735251590706	36.0	36.0	36.0	36.0	36.0
13	35.2932721438479	36.0	36.0	36.0	36.0	36.0
14	35.27104971355884	36.0	36.0	36.0	36.0	36.0
15	35.233514752756406	36.0	36.0	36.0	36.0	36.0
16	35.258178252943	36.0	36.0	36.0	36.0	36.0
17	35.26760011396118	36.0	36.0	36.0	36.0	36.0
18	35.267782023827515	36.0	36.0	36.0	36.0	36.0
19	35.20478636960072	36.0	36.0	36.0	36.0	36.0
20	35.17699428722765	36.0	36.0	36.0	36.0	36.0
21	35.119061345089705	36.0	36.0	36.0	36.0	36.0
22	35.0809271517864	36.0	36.0	36.0	36.0	36.0
23	35.038657184169026	36.0	36.0	36.0	36.0	36.0
24	35.03190244280874	36.0	36.0	36.0	36.0	36.0
25	34.97393338621203	36.0	36.0	36.0	36.0	36.0
26	34.93809580497122	36.0	36.0	36.0	36.0	36.0
27	34.87315398268914	36.0	36.0	36.0	36.0	36.0
28	34.806228540320454	36.0	36.0	36.0	36.0	36.0
29	34.75603211779199	36.0	36.0	36.0	32.0	36.0
30	34.68946915758343	36.0	36.0	36.0	32.0	36.0
31	34.66886519007575	36.0	36.0	36.0	32.0	36.0
32	34.6237622438047	36.0	36.0	36.0	32.0	36.0
33	34.61827150850094	36.0	36.0	36.0	32.0	36.0
34	34.57612192241277	36.0	36.0	36.0	32.0	36.0
35	34.50118308291746	36.0	36.0	36.0	32.0	36.0
36	34.413997429185564	36.0	36.0	36.0	32.0	36.0
37	34.28564664275979	36.0	36.0	36.0	32.0	36.0
38	34.20253389743226	36.0	36.0	36.0	32.0	36.0
39	33.93929962026316	36.0	36.0	36.0	32.0	36.0
40	33.956499465639766	36.0	36.0	36.0	32.0	36.0
41	33.95157719866831	36.0	36.0	36.0	32.0	36.0
42	33.81771828849567	36.0	36.0	36.0	32.0	36.0
43	33.84185879781655	36.0	36.0	36.0	32.0	36.0
44	33.7752931624629	36.0	36.0	36.0	32.0	36.0
45	33.604376804886954	36.0	36.0	36.0	21.0	36.0
46	33.57715185327364	36.0	36.0	36.0	21.0	36.0
47	33.51754293273481	36.0	36.0	36.0	21.0	36.0
48	33.47531041714875	36.0	36.0	36.0	21.0	36.0
49	33.46407614532993	36.0	36.0	36.0	21.0	36.0
50	32.9888152183654	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	14.0
22	67.0
23	205.0
24	605.0
25	1515.0
26	3393.0
27	6604.0
28	11165.0
29	16019.0
30	22253.0
31	29614.0
32	42984.0
33	58756.0
34	116689.0
35	437734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.801974873276066	19.92224655547692	12.903031608407325	25.372746962839688
2	10.364449152882447	22.032547077691902	47.317929250368515	20.285074519057137
3	20.637835697718902	26.913600723360197	29.884620837803062	22.563942741117838
4	10.505350987601974	18.328453396716775	39.98855031774206	31.17764529793919
5	9.742075885840858	41.21061016046858	37.071090643278765	11.976223310411799
6	29.607995516432833	38.653244500206654	19.39717957943819	12.341580403922318
7	24.966861640158207	32.21824368699197	23.28499792007469	19.529896752775127
8	22.107934079074614	43.80322702752591	21.27516141156706	12.81367748183242
9	26.01377967237498	15.477051936604411	20.40921694490405	38.099951446116556
10	14.28741491366638	31.23058011858918	36.15859865199439	18.323406315750052
11	33.88017757613129	20.532942405463718	28.002081262882495	17.584798755522502
12	23.98749102154428	23.90442776640098	35.960504157844255	16.147577054210476
13	32.27241537512891	22.997553579812287	26.280357880910564	18.44967316414824
14	19.282445831655796	25.586559000993812	27.342390482903816	27.78860468444657
15	20.94384469177647	37.7469660510712	24.47195979791954	16.837229459232795
16	18.74099646479576	27.822043998111347	35.64764593919663	17.789313597896268
17	18.168649172109472	30.928021208550298	32.49177727276983	18.4115523465704
18	18.766677857690304	27.115136907238007	38.8215718350024	15.296613400069287
19	23.599193711268914	27.16970986713892	27.323798224506202	21.907298197085964
20	22.181634326391777	29.382054859200423	32.31133873623471	16.124972078173087
21	20.266765468692107	28.127010538734094	26.28276551149443	25.32345848107937
22	20.620954679029406	33.431154472240685	28.011444270708637	17.936446578021275
23	18.903244014697247	32.19523743919061	29.10062959539768	19.800888950714466
24	22.538899953586235	29.441041808505087	30.552163322958233	17.46789491495045
25	21.647942880301972	30.028637428222517	30.187674804012183	18.135744887463336
26	15.557172532145211	31.933340734568088	33.181295920537494	19.328190812749206
27	18.495150630732336	31.74915699490251	31.815500593213425	17.94019178115173
28	17.480200582379087	32.204466689762086	28.131959557156485	22.183373170702346
29	18.950727840101226	28.864681798179028	28.514371548226848	23.670218813492898
30	18.837034173640994	29.56142333769828	30.59924587659823	21.002296612062494
31	21.45479740457423	27.4575554791653	29.961090014619668	21.1265571016408
32	18.523774683229384	31.989786296034232	30.623990968710164	18.86244805202622
33	17.907822525524228	31.31377713098714	32.73441293272144	18.043987410767194
34	17.991688324195483	32.425299917204256	33.0491437529343	16.53386800566596
35	18.101904302034583	32.883284757157014	31.593864822243294	17.420946118565105
36	16.90664947440087	31.891207199350475	32.68345141869632	18.518691907552338
37	18.070337589935033	30.97443497591701	29.212049388528712	21.74317804561925
38	19.83419450712458	33.475963152551486	27.14121957189653	19.548622768427403
39	21.20212995052319	27.982953975466245	30.537985053964366	20.2769310200462
40	24.486405581422723	28.69266996868743	29.51367199778498	17.30725245210487
41	21.46696931474821	26.188199132450446	33.861050288715035	18.48378126408631
42	20.94651983686965	32.73989698016246	30.6546213800271	15.658961802940787
43	17.51083099369602	31.818309495561266	31.83008013397127	18.840779376771447
44	16.90932461949405	32.229613053638	31.434693689198966	19.426368637668986
45	17.804428167672746	32.5471527761987	30.212821167888094	19.435597888240462
46	18.54963945635556	27.643150740816537	31.344223878141463	22.46298592468644
47	22.844535280482276	28.127813082262048	30.320495757888665	18.707155879367008
48	17.529690766602954	31.123306800352584	28.464480092239004	22.88252234080546
49	20.457235799326668	28.5151740917548	31.403662006118054	19.623928102800477
50	17.64739715070296	34.42711099043234	26.549611234539334	21.37588062432536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	12.5
2	16.0
3	50.0
4	84.0
5	105.5
6	127.0
7	126.5
8	126.0
9	162.0
10	198.0
11	323.5
12	449.0
13	876.5
14	1304.0
15	1821.5
16	2339.0
17	2887.0
18	3435.0
19	3858.5
20	4282.0
21	4911.0
22	5540.0
23	6148.5
24	6757.0
25	8541.0
26	10325.0
27	17050.0
28	23775.0
29	28964.5
30	34154.0
31	40059.5
32	45965.0
33	56945.0
34	67925.0
35	79416.0
36	90907.0
37	88946.5
38	86986.0
39	76229.0
40	65472.0
41	56514.5
42	47557.0
43	42599.0
44	37641.0
45	39720.5
46	41800.0
47	44141.0
48	46482.0
49	41219.0
50	35956.0
51	30020.0
52	24084.0
53	20535.5
54	16987.0
55	15174.5
56	13362.0
57	11808.0
58	10254.0
59	8865.5
60	7477.0
61	6343.5
62	5210.0
63	4367.0
64	3524.0
65	2965.5
66	2407.0
67	2002.5
68	1598.0
69	1368.0
70	1138.0
71	944.5
72	751.0
73	646.0
74	541.0
75	437.0
76	333.0
77	263.5
78	194.0
79	134.5
80	75.0
81	56.5
82	38.0
83	26.5
84	15.0
85	14.5
86	14.0
87	9.5
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017655957615001144
2	0.0038789603851138877
3	1.3375725465909958E-4
4	5.350290186363983E-4
5	0.0
6	5.350290186363983E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.6751450931819916E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	747623.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.344455929687335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09591036745293	45.93056402214591
2	12.25734534768075	14.057816002100736
3	3.5037719619758487	6.027656905835945
4	1.4103368316643075	3.2349999315755493
5	0.7053491146643567	2.022393061045709
6	0.41258482602104163	1.41956714238128
7	0.2791550710965074	1.120559697042968
8	0.19849222300632402	0.9105942827657454
9	0.1430971262793971	0.738524416643441
>10	0.8495911124058585	9.124935848697367
>50	0.07546428062085979	2.9848670435882596
>100	0.06046478972864298	6.764097153598773
>500	0.005390271952066617	2.2306428354290437
>1k	0.0030466754511680877	3.432781657149299
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3766	0.5037298210461689	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3343	0.4471505023253699	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2703	0.36154585934354616	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2024	0.27072468343001754	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1933	0.2585527732560395	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1821	0.24357196073422033	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1520	0.20331102708183135	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1459	0.19515183454762627	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1450	0.19394801925569438	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	1445	0.19327923298239888	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1370	0.1832474388829664	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1366	0.18271240986433002	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1343	0.17963599300717073	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	954	0.12760442094478097	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	921	0.12319043154103071	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	915	0.12238788801307611	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	914	0.12225413075841701	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	869	0.11623505429875752	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	844	0.11289112293228004	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	843	0.11275736567762093	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	827	0.11061724960307534	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	783	0.10473193039807498	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	755	0.10098672726762017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.3375725465909958E-4	0.0
16	0.0	0.0	0.0	4.012717639772987E-4	0.0
17	0.0	0.0	0.0	4.012717639772987E-4	0.0
18	0.0	0.0	0.0	4.012717639772987E-4	0.0
19	0.0	0.0	0.0	5.350290186363983E-4	0.0
20	0.0	0.0	0.0	9.36300782613697E-4	0.0
21	0.0	0.0	0.0	0.0010700580372727966	0.0
22	0.0	0.0	0.0	0.0021401160745455933	0.0
23	0.0	0.0	0.0	0.004949018422386684	0.0
24	0.0	0.0	0.0	0.008159192534205073	0.0
25	0.0	0.0	0.0	0.009898036844773368	0.0
26	0.0	0.0	0.0	0.01270693919261446	0.0
27	0.0	0.0	0.0	0.016987171341705645	0.0
28	0.0	0.0	0.0	0.025681392894547118	0.0
29	0.0	0.0	0.0	0.03598070150329778	0.0
30	0.0	0.0	0.0	0.055107988919549024	0.0
31	0.0	0.0	0.0	0.10152175628625658	0.0
32	0.0	0.0	0.0	0.1613112491188741	0.0
33	0.0	0.0	0.0	0.2156166945104685	0.0
34	0.0	0.0	0.0	0.2822278073307001	0.0
35	0.0	0.0	0.0	0.35566054013854576	0.0
36	0.0	0.0	0.0	0.45597848113287043	0.0
37	0.0	0.0	0.0	0.6176910020157218	0.0
38	0.0	0.0	0.0	0.7991995965881199	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	30	1.3007413E-4	36.666275	6
TCGTATA	40	1.8192814E-5	32.99965	44
TACGGCG	45	4.067787E-5	29.333021	16
GTCGTAT	60	2.9431067E-7	29.333021	43
TCGTATC	30	0.005743927	29.333021	44
TAGGACC	1305	0.0	27.815796	4
CGTGTAA	40	7.030044E-4	27.499706	9
ACGATTT	60	9.747284E-6	25.666393	29
CTAGAGT	190	0.0	25.473413	4
GACGTGT	45	0.0013978019	24.444185	7
TCACGAT	55	1.59294E-4	23.999746	27
ACGGCGA	55	1.59294E-4	23.999746	17
CACGATT	65	1.8123405E-5	23.692055	28
TGTAGGA	3580	0.0	23.354832	2
CTGTAGG	3495	0.0	23.297958	1
GGACCTG	2315	0.0	22.61747	6
ATACACT	1510	0.0	22.58254	4
AGGACCT	2420	0.0	22.545214	5
ACTGTTC	1370	0.0	22.481512	8
ATAGGAC	265	0.0	22.414856	3
>>END_MODULE