FastQCFastQC Report
Thu 2 Feb 2017
SRR4062421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606962
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG92240.5740023721780602No Hit
TCCATGTACTCTGCGTTGATACCAC79660.49571800702194574No Hit
GAGTACATGGAAGCAGTGGTATCAA38520.2397069750249228No Hit
CATGTACTCTGCGTTGATACCACTG34600.2153131187918569No Hit
GTATCAACGCAGAGTACTTTTTTTT31330.19496416218927393No Hit
GCTTCCATGTACTCTGCGTTGATAC25900.16117369296847092No Hit
CCCATGTACTCTGCGTTGATACCAC23730.14766995112516662No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21980.13677983673540509No Hit
CATGGAAGCAGTGGTATCAACGCAG21190.1318637279537413No Hit
GTCCTACAGTGGACATTTCTAAATT21100.13130366492798212No Hit
TATCAACGCAGAGTACTTTTTTTTT21080.13117920647781342No Hit
GCGTTGATACCACTGCTTCCATGTA21060.13105474802764472No Hit
GTACATGGGAAGCAGTGGTATCAAC19880.12371169946769121No Hit
CTGTAGGACGTGGAATATGGCAAGA19010.11829775688535261No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18810.11705317238366558No Hit
GTCCTAAAGTGTGTATTTCTCATTT18640.1159952755572316No Hit
GTATCAACGCAGAGTACATGGAAGC17770.11058133297489299No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17730.11033241607455559No Hit
GAATAGGACCGCGGTTCTATTTTGT17600.10952343614845901No Hit
GCGCAAGACGGACCAGAGCGAAAGC17320.10778101784609717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT655.4510274E-513.1542776
ATCGCCA2050.012.97765316
GTCTTAG1403.6379788E-1212.8864531
CGCATCG2150.012.81437413
ATCCCCG1501.8189894E-1212.66747812
AATCGTA751.4781555E-512.66708213
CGGCTAA1001.4419129E-712.3504059
CATCGCC2250.012.24484615
GTATTAG2950.012.231211
GTGTTAA701.0960148E-412.2082191
AAGACGG3200.012.1718955
AGCGCAT550.003067765912.09130710
AGAGCGA3300.012.09130615
GTATTAT1103.8402504E-812.0849041
CGTAAAT802.8618811E-511.8768716
GTATAGG1459.822543E-1111.7872461
CGCCAGT2450.011.6352118
GCATCGC2300.011.56559814
ATTTGCG2400.011.48097416
AATTCCG1251.8313585E-811.4000195
Produced by FastQC (version 0.11.5)