##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062421_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1606962
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18987070011612	32.0	32.0	32.0	32.0	32.0
2	30.886660045477118	32.0	32.0	32.0	32.0	32.0
3	30.878832853546008	32.0	32.0	32.0	32.0	32.0
4	30.9311682541342	32.0	32.0	32.0	32.0	32.0
5	30.76128371423842	32.0	32.0	32.0	32.0	32.0
6	34.39693222366179	36.0	36.0	36.0	32.0	36.0
7	34.28260966967483	36.0	36.0	36.0	32.0	36.0
8	34.287017365687554	36.0	36.0	36.0	32.0	36.0
9	34.427400896847594	36.0	36.0	36.0	32.0	36.0
10	34.133344161218496	36.0	36.0	36.0	32.0	36.0
11	34.41642303925046	36.0	36.0	36.0	32.0	36.0
12	34.20968697455198	36.0	36.0	36.0	32.0	36.0
13	34.31678844926016	36.0	36.0	36.0	32.0	36.0
14	34.22726735293056	36.0	36.0	36.0	32.0	36.0
15	34.17710499688231	36.0	36.0	36.0	32.0	36.0
16	34.17842861249986	36.0	36.0	36.0	32.0	36.0
17	34.10299185668361	36.0	36.0	36.0	32.0	36.0
18	34.12245715828999	36.0	36.0	36.0	32.0	36.0
19	34.1047249406022	36.0	36.0	36.0	32.0	36.0
20	34.098135487958025	36.0	36.0	36.0	32.0	36.0
21	34.06399902424575	36.0	36.0	36.0	32.0	36.0
22	34.01395179226391	36.0	36.0	36.0	32.0	36.0
23	33.96534578913503	36.0	36.0	36.0	32.0	36.0
24	33.943078305523095	36.0	36.0	36.0	32.0	36.0
25	33.46393816406362	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	65.0
5	152.0
6	259.0
7	93.0
8	220.0
9	275.0
10	187.0
11	63.0
12	103.0
13	77.0
14	264.0
15	362.0
16	529.0
17	723.0
18	1038.0
19	1381.0
20	2071.0
21	2751.0
22	4184.0
23	6041.0
24	8911.0
25	12132.0
26	17389.0
27	22642.0
28	31330.0
29	42888.0
30	56482.0
31	78319.0
32	112979.0
33	164536.0
34	364685.0
35	673826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44498401514034	17.490367698447816	11.415685388587365	25.64896289782448
2	15.689893690176222	19.986595366128842	38.58306672383654	25.7404442198584
3	19.368184440407667	24.07602746418088	28.457090795715708	28.098697299695747
4	12.136715112950045	15.582724642876702	36.189137178165815	36.09142306600744
5	13.808178894088222	37.0009891664794	34.30278703413776	14.888044905294622
6	34.10947450469638	35.35823169308141	17.169819895818613	13.362473906403599
7	29.60171913169154	30.501448695986806	21.320956681094472	18.575875491227183
8	27.53304101944469	34.22788026756686	19.87337909042215	18.3656996225663
9	26.74569687728517	14.724546579115543	18.862047186417367	39.66770935718192
10	15.39027518969093	27.20341459251105	32.52946369441116	24.876846523386856
11	37.0420881137136	20.984122336200265	22.742845394480664	19.230944155605467
12	24.20149479252666	23.729940401456783	29.85011573893782	22.218449067078737
13	29.865288860755708	19.834598495760915	25.558793917251897	24.741318726231476
14	22.899278513663383	20.593450548902815	25.225480056323285	31.281790881110517
15	24.775101982840468	28.504421956027144	22.991056410154176	23.729419650978212
16	25.09190380579251	25.771876719010773	25.72814415051978	23.408075324676943
17	23.07865798573253	26.55306688265163	26.79087878882278	23.577396342793058
18	24.28985503634372	24.53827337107481	28.594838232123788	22.577033360457683
19	24.956258738905795	26.062376782986018	25.479222277885366	23.50214220022282
20	24.618815110453745	24.835816208854368	26.84483642677755	23.700532253914336
21	25.461266798261356	25.18025730749292	24.393866889978867	24.96460900426686
22	24.480034599962856	26.105086169612107	25.390527138411066	24.024352092013963
23	23.00614968746826	25.535940059708484	26.015564585427313	25.442345667395937
24	23.91480176617718	26.30466182542363	25.96113463050731	23.819401777891876
25	24.445892765015664	25.322382866727644	25.718040231188517	24.51368413706817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	477.5
3	789.0
4	789.0
5	789.0
6	2287.0
7	3785.0
8	3785.0
9	3785.0
10	3969.5
11	4154.0
12	4154.0
13	4154.0
14	4577.0
15	5000.0
16	5000.0
17	5000.0
18	8006.5
19	11013.0
20	11013.0
21	11013.0
22	18674.5
23	26336.0
24	26336.0
25	26336.0
26	40708.5
27	55081.0
28	55081.0
29	55081.0
30	70436.5
31	85792.0
32	85792.0
33	85792.0
34	99977.5
35	114163.0
36	114163.0
37	114163.0
38	125348.5
39	136534.0
40	136534.0
41	136534.0
42	154783.0
43	173032.0
44	173032.0
45	173032.0
46	207230.0
47	241428.0
48	241428.0
49	241428.0
50	224900.5
51	208373.0
52	208373.0
53	208373.0
54	186244.5
55	164116.0
56	164116.0
57	164116.0
58	149798.0
59	135480.0
60	135480.0
61	135480.0
62	118980.0
63	102480.0
64	102480.0
65	102480.0
66	84410.0
67	66340.0
68	66340.0
69	66340.0
70	49769.0
71	33198.0
72	33198.0
73	33198.0
74	25714.0
75	18230.0
76	18230.0
77	18230.0
78	15372.0
79	12514.0
80	12514.0
81	12514.0
82	8680.5
83	4847.0
84	4847.0
85	4847.0
86	3572.0
87	2297.0
88	2297.0
89	2297.0
90	1609.0
91	921.0
92	921.0
93	921.0
94	549.0
95	177.0
96	177.0
97	177.0
98	446.5
99	716.0
100	716.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00802757003588137
2	0.003422607379639344
3	0.004293816530820268
4	0.014561638669738301
5	0.034786136822152605
6	0.045116188186154996
7	0.06440724796230403
8	0.08600078906657407
9	0.09334383762652758
10	0.11518629563113503
11	0.11680425548332818
12	0.12800551599851148
13	0.11811106921009956
14	0.12084915511381103
15	0.11139031290098957
16	0.10896337312269984
17	0.11898227836128047
18	0.12495628396937825
19	0.12881449592460806
20	0.11829775688535261
21	0.1265742438215714
22	0.1453674697970456
23	0.13472627230762146
24	0.13317054168051268
25	0.1268231607219088
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1606962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.559380401133126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75214864287376	39.02907691998761
2	12.834481982220181	12.72137949616678
3	3.8210573589056893	5.681077055536685
4	1.549709215483487	3.072105140851509
5	0.7711013955164591	1.91076536941224
6	0.47995564743292357	1.4271782704080236
7	0.3049135300364316	1.0577927937169493
8	0.21851396423297628	0.8663533341105334
9	0.16349228906936025	0.7292318891976399
>10	0.8848229791783124	8.268954341485545
>50	0.09583036586463398	3.3305136322436044
>100	0.10211739335075262	10.651395089918626
>500	0.015198181241659683	5.012161957988655
>1k	0.006405844986154082	5.171955044579938
>5k	2.5120960730016004E-4	1.0700596643958493
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	9224	0.5740023721780602	No Hit
TCCATGTACTCTGCGTTGATACCAC	7966	0.49571800702194574	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3852	0.2397069750249228	No Hit
CATGTACTCTGCGTTGATACCACTG	3460	0.2153131187918569	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3133	0.19496416218927393	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2590	0.16117369296847092	No Hit
CCCATGTACTCTGCGTTGATACCAC	2373	0.14766995112516662	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2198	0.13677983673540509	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2119	0.1318637279537413	No Hit
GTCCTACAGTGGACATTTCTAAATT	2110	0.13130366492798212	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2108	0.13117920647781342	No Hit
GCGTTGATACCACTGCTTCCATGTA	2106	0.13105474802764472	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1988	0.12371169946769121	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1901	0.11829775688535261	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1881	0.11705317238366558	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1864	0.1159952755572316	No Hit
GTATCAACGCAGAGTACATGGAAGC	1777	0.11058133297489299	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1773	0.11033241607455559	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1760	0.10952343614845901	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1732	0.10778101784609717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.222922508435171E-5	6.222922508435171E-5
2	0.0	0.0	0.0	6.222922508435171E-5	6.222922508435171E-5
3	0.0	0.0	0.0	6.222922508435171E-5	6.222922508435171E-5
4	0.0	0.0	0.0	6.222922508435171E-5	6.222922508435171E-5
5	0.0	0.0	0.0	6.222922508435171E-5	6.222922508435171E-5
6	0.0	0.0	0.0	6.222922508435171E-5	1.8668767525305516E-4
7	0.0	0.0	0.0	6.222922508435171E-5	1.8668767525305516E-4
8	0.0	0.0	0.0	6.222922508435171E-5	1.8668767525305516E-4
9	0.0	0.0	0.0	1.2445845016870343E-4	1.8668767525305516E-4
10	0.0	0.0	0.0	1.8668767525305516E-4	1.8668767525305516E-4
11	0.0	0.0	0.0	1.8668767525305516E-4	1.8668767525305516E-4
12	0.0	0.0	0.0	2.4891690033740686E-4	3.733753505061103E-4
13	0.0	0.0	0.0	2.4891690033740686E-4	3.733753505061103E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	65	5.4510274E-5	13.154277	6
ATCGCCA	205	0.0	12.977653	16
GTCTTAG	140	3.6379788E-12	12.886453	1
CGCATCG	215	0.0	12.814374	13
ATCCCCG	150	1.8189894E-12	12.667478	12
AATCGTA	75	1.4781555E-5	12.667082	13
CGGCTAA	100	1.4419129E-7	12.350405	9
CATCGCC	225	0.0	12.244846	15
GTATTAG	295	0.0	12.23121	1
GTGTTAA	70	1.0960148E-4	12.208219	1
AAGACGG	320	0.0	12.171895	5
AGCGCAT	55	0.0030677659	12.091307	10
AGAGCGA	330	0.0	12.091306	15
GTATTAT	110	3.8402504E-8	12.084904	1
CGTAAAT	80	2.8618811E-5	11.87687	16
GTATAGG	145	9.822543E-11	11.787246	1
CGCCAGT	245	0.0	11.63521	18
GCATCGC	230	0.0	11.565598	14
ATTTGCG	240	0.0	11.480974	16
AATTCCG	125	1.8313585E-8	11.400019	5
>>END_MODULE