FastQCFastQC Report
Thu 2 Feb 2017
SRR4062421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606962
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58670.3650988635698915No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC46180.2873745614395362No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA21240.13217487407916306No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20790.12937455895036723No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19250.11979125828737705No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18150.11294604352809837No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17940.11163922980132698No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17890.11132808367590522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA4800.021.99984636
TGTAGGA16300.020.6514452
CAATACG5300.019.92438738
GTAGGAC16200.019.8282553
CGGGATT1900.019.68407244
AATACGA5600.019.64271739
CTGTAGG16600.019.2185251
TAGCGGC5750.019.13029930
GCAATAC5750.019.13029937
CTAGCGG5750.019.13029929
TCTAGCG5650.019.07951228
GTCCTAA7400.019.028671
CGTCTTA2800.018.8570115
ATACGAA5500.018.79986840
AGGACCT12350.018.1704775
TAGGACA6450.018.0779534
CGAATGC5600.018.071343
GGACCTG12350.017.8141946
TACGAAT6000.017.59987441
GCGAGAA10400.017.5575720
Produced by FastQC (version 0.11.5)