##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1606962
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27988154044713	32.0	32.0	32.0	32.0	32.0
2	31.40081719418381	32.0	32.0	32.0	32.0	32.0
3	31.500736171732747	32.0	32.0	32.0	32.0	32.0
4	31.606103940230074	32.0	32.0	32.0	32.0	32.0
5	31.535775581500992	32.0	32.0	32.0	32.0	32.0
6	35.140433314540104	36.0	36.0	36.0	36.0	36.0
7	35.15290156207801	36.0	36.0	36.0	36.0	36.0
8	35.09267113970336	36.0	36.0	36.0	36.0	36.0
9	35.229733497120655	36.0	36.0	36.0	36.0	36.0
10	35.102017969311035	36.0	36.0	36.0	36.0	36.0
11	35.23778222509306	36.0	36.0	36.0	36.0	36.0
12	35.14403265291899	36.0	36.0	36.0	36.0	36.0
13	35.18652774614459	36.0	36.0	36.0	36.0	36.0
14	35.151963767655985	36.0	36.0	36.0	36.0	36.0
15	35.113741955316925	36.0	36.0	36.0	36.0	36.0
16	35.12047391288655	36.0	36.0	36.0	36.0	36.0
17	35.098203317813365	36.0	36.0	36.0	36.0	36.0
18	35.11407052562537	36.0	36.0	36.0	36.0	36.0
19	35.089799883257974	36.0	36.0	36.0	36.0	36.0
20	35.081568201363815	36.0	36.0	36.0	36.0	36.0
21	35.06303820501045	36.0	36.0	36.0	36.0	36.0
22	35.04047824404062	36.0	36.0	36.0	36.0	36.0
23	35.00116057504782	36.0	36.0	36.0	36.0	36.0
24	34.976130736134394	36.0	36.0	36.0	36.0	36.0
25	34.94731300429008	36.0	36.0	36.0	32.0	36.0
26	34.90437110522838	36.0	36.0	36.0	32.0	36.0
27	34.88217394064079	36.0	36.0	36.0	32.0	36.0
28	34.85419443645836	36.0	36.0	36.0	32.0	36.0
29	34.82374941037809	36.0	36.0	36.0	32.0	36.0
30	34.80311108787887	36.0	36.0	36.0	32.0	36.0
31	34.79574190304438	36.0	36.0	36.0	32.0	36.0
32	34.76491790098335	36.0	36.0	36.0	32.0	36.0
33	34.739899263330436	36.0	36.0	36.0	32.0	36.0
34	34.72419136233464	36.0	36.0	36.0	32.0	36.0
35	34.67971924662811	36.0	36.0	36.0	32.0	36.0
36	34.64342778485117	36.0	36.0	36.0	32.0	36.0
37	34.616595165287045	36.0	36.0	36.0	32.0	36.0
38	34.557369122605266	36.0	36.0	36.0	32.0	36.0
39	34.49014351303889	36.0	36.0	36.0	32.0	36.0
40	34.45473010562789	36.0	36.0	36.0	32.0	36.0
41	34.44008010145853	36.0	36.0	36.0	32.0	36.0
42	34.370009371721295	36.0	36.0	36.0	32.0	36.0
43	34.37346371600573	36.0	36.0	36.0	32.0	36.0
44	34.3574689382823	36.0	36.0	36.0	32.0	36.0
45	34.25099784562423	36.0	36.0	36.0	32.0	36.0
46	34.28365325377949	36.0	36.0	36.0	32.0	36.0
47	34.243107802175786	36.0	36.0	36.0	32.0	36.0
48	34.21207222074946	36.0	36.0	36.0	32.0	36.0
49	34.216772394120085	36.0	36.0	36.0	32.0	36.0
50	33.66773452016911	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	28.0
22	108.0
23	339.0
24	952.0
25	2274.0
26	4786.0
27	9107.0
28	16063.0
29	25651.0
30	38033.0
31	56010.0
32	85717.0
33	136103.0
34	288263.0
35	943518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.017309723431545	18.044633593634764	11.935194964670414	26.002861718263276
2	15.169886750463938	20.495502503587666	38.13412707994429	26.200483666004104
3	18.794163605486414	24.401304084567606	28.798388998793367	28.00614331115261
4	11.94163876195816	16.103915661713412	36.37129058213755	35.583154994190885
5	13.652905295831513	37.35284343998178	34.07815492836794	14.916096335818768
6	34.19326092733035	35.535238341785124	16.973696247005986	13.297804483878537
7	29.66050223962981	30.66892683212173	21.037709665816614	18.632861262431845
8	27.6180768431363	34.41587293290071	19.646886485181355	18.319163738781626
9	26.961185143145887	14.465681204658232	18.666713960877733	39.90641969131815
10	15.471865545047114	27.321367897934113	32.381910711018676	24.824855846000094
11	37.27686155615378	20.920283118082445	22.604579324215507	19.198276001548262
12	24.35191373535902	23.82196965454068	29.607296252182692	22.21882035791761
13	30.10537896975784	19.729277979193036	25.4006628657056	24.764680185343522
14	22.88479752477034	20.604967634580035	25.117146516221293	31.39308832442833
15	24.826909410427874	28.62488347577603	22.75162698308983	23.796580130706264
16	25.184665225437815	25.86601301088638	25.494566766357885	23.45475499731792
17	23.251763265092766	26.642571510714006	26.467147325201218	23.638517898992013
18	24.59846592514322	24.616574629642766	28.302473860614004	22.48248558460001
19	25.039484443316024	26.12382869041085	25.29543324608796	23.541253620185167
20	24.96182237041075	24.883351317579383	26.554952761795235	23.599873550214628
21	25.703656962641308	25.27327964195793	24.181094512502472	24.84196888289829
22	24.547064585223545	26.140319435058203	25.18317172403579	24.12944425568246
23	23.123322144518664	25.546279252402982	25.756489574737923	25.573909028340434
24	24.118803058193038	26.282139839025444	25.712929117178874	23.886127985602645
25	24.731760925273903	25.273528558858267	25.447272555293775	24.547437960574054
26	23.06103069020923	26.20646910132287	26.57729305360052	24.155207154867384
27	24.594981088538496	26.076970083922333	25.65045097519419	23.677597852344984
28	23.486118526760432	25.458287128133712	25.887731010440817	25.167863334665036
29	23.42158682034796	25.766072875400916	26.152765280075073	24.659575024176053
30	24.04002085923625	25.5879728332095	26.434788128157354	23.9372181793969
31	24.282963753965557	25.290205991180876	25.460651838686914	24.966178416166652
32	23.609145704752198	26.080890525102646	25.54080308059556	24.769160689549597
33	23.03800587692802	25.650699892094526	26.386311561816644	24.924982669160816
34	23.685189817805274	26.19620127918395	26.891861786401915	23.226747116608855
35	24.822802281572308	25.755680594811825	26.23920167371724	23.182315449898628
36	23.408456453855162	26.36334897776052	26.07323633041727	24.154958237967044
37	24.75042969279921	25.921957084237214	25.417775902603797	23.909837320359784
38	23.848541533651698	26.117481309452245	25.397240258325958	24.636736898570096
39	24.525471044119275	25.314537618188854	25.65947421283142	24.50051712486045
40	25.453059873226625	25.551755424210405	25.579509658598027	23.41567504396495
41	23.655444248214955	25.478947230861714	26.919864937689873	23.945743583233455
42	25.515164639860803	26.578288721201872	25.647401743165055	22.25914489577227
43	24.022161071637036	25.08995234485943	26.103728650708604	24.784157932794926
44	23.498813288677642	25.89127807627063	26.310516365663904	24.299392269387827
45	23.903241022500843	26.15562782442895	25.898683354055663	24.04244779901454
46	23.630208592622093	25.181273958281476	26.521506446955744	24.667011002140686
47	24.83082985160819	24.860948796549014	26.62744980901851	23.680771542824285
48	24.424908616382965	26.782089433353125	24.847507283930796	23.94549466633312
49	23.706534442009207	25.738194182563124	26.485691634276353	24.069579741151315
50	23.34630398621995	27.36606551123518	24.645588785290993	24.642041717253875
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	20.5
2	26.0
3	85.0
4	144.0
5	170.0
6	196.0
7	171.5
8	147.0
9	242.0
10	337.0
11	499.0
12	661.0
13	1167.0
14	1673.0
15	2223.0
16	2773.0
17	3390.0
18	4007.0
19	4461.5
20	4916.0
21	5961.0
22	7006.0
23	7847.0
24	8688.0
25	10405.5
26	12123.0
27	16416.0
28	20709.0
29	24176.5
30	27644.0
31	31457.5
32	35271.0
33	41745.5
34	48220.0
35	55819.5
36	63419.0
37	70197.5
38	76976.0
39	78199.5
40	79423.0
41	82164.0
42	84905.0
43	85808.0
44	86711.0
45	98900.0
46	111089.0
47	121290.0
48	131491.0
49	129285.0
50	127079.0
51	116068.0
52	105057.0
53	101157.0
54	97257.0
55	98616.5
56	99976.0
57	96397.0
58	92818.0
59	84747.0
60	76676.0
61	67211.5
62	57747.0
63	49998.0
64	42249.0
65	36162.5
66	30076.0
67	25724.0
68	21372.0
69	19986.0
70	18600.0
71	14227.0
72	9854.0
73	8984.0
74	8114.0
75	5711.0
76	3308.0
77	3054.5
78	2801.0
79	2454.5
80	2108.0
81	1691.5
82	1275.0
83	1101.5
84	928.0
85	791.5
86	655.0
87	483.0
88	311.0
89	192.5
90	74.0
91	47.5
92	21.0
93	20.5
94	20.0
95	15.0
96	10.0
97	6.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014623867894822652
2	0.004231587305735917
3	6.845214759278688E-4
4	3.111461254217586E-4
5	0.0
6	3.111461254217586E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2445845016870343E-4
47	0.0
48	0.0
49	0.0
50	6.222922508435171E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1606962.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.8783218540705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43951668121326	42.8006784768221
2	12.640740008553353	13.621237173089268
3	3.620231235606747	5.851559510945388
4	1.4603414987914753	3.147229971549712
5	0.7449299273895511	2.00677871933118
6	0.46316625407609396	1.4972772305433586
7	0.28963944148834425	1.092370093509956
8	0.20680746263574626	0.8913951226969915
9	0.1481276769892676	0.7182783590691197
>10	0.8010096247946247	7.992503437533065
>50	0.08657308492853795	3.2684009806195533
>100	0.08463696793929755	9.859635397131106
>500	0.010216682376003313	3.774200718801921
>1k	0.003947354554364917	3.1114621567967444
>5k	1.1609866336367402E-4	0.3669926515605826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5867	0.3650988635698915	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4618	0.2873745614395362	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2124	0.13217487407916306	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2079	0.12937455895036723	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1925	0.11979125828737705	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1815	0.11294604352809837	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1794	0.11163922980132698	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1789	0.11132808367590522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2445845016870343E-4
2	0.0	0.0	0.0	0.0	1.2445845016870343E-4
3	0.0	0.0	0.0	0.0	1.2445845016870343E-4
4	0.0	0.0	0.0	0.0	1.2445845016870343E-4
5	0.0	0.0	0.0	0.0	1.2445845016870343E-4
6	0.0	0.0	0.0	0.0	1.2445845016870343E-4
7	0.0	0.0	0.0	6.222922508435171E-5	1.2445845016870343E-4
8	0.0	0.0	0.0	6.222922508435171E-5	1.2445845016870343E-4
9	0.0	0.0	0.0	6.222922508435171E-5	1.2445845016870343E-4
10	0.0	0.0	0.0	6.222922508435171E-5	1.2445845016870343E-4
11	0.0	0.0	0.0	6.222922508435171E-5	1.2445845016870343E-4
12	0.0	0.0	0.0	6.222922508435171E-5	3.733753505061103E-4
13	0.0	0.0	0.0	6.222922508435171E-5	5.600630257591654E-4
14	0.0	0.0	0.0	1.2445845016870343E-4	5.600630257591654E-4
15	0.0	0.0	0.0	1.2445845016870343E-4	6.845214759278688E-4
16	0.0	0.0	0.0	1.2445845016870343E-4	6.845214759278688E-4
17	0.0	0.0	0.0	1.2445845016870343E-4	6.845214759278688E-4
18	0.0	0.0	0.0	1.8668767525305516E-4	7.467507010122206E-4
19	0.0	0.0	0.0	2.4891690033740686E-4	7.467507010122206E-4
20	0.0	0.0	0.0	3.733753505061103E-4	7.467507010122206E-4
21	0.0	0.0	0.0	4.978338006748137E-4	8.089799260965723E-4
22	0.0	0.0	0.0	6.845214759278688E-4	9.334383762652757E-4
23	0.0	0.0	0.0	0.0012445845016870343	9.334383762652757E-4
24	0.0	0.0	0.0	0.00217802287795231	9.334383762652757E-4
25	0.0	0.0	0.0	0.0025513982284584204	9.334383762652757E-4
26	0.0	0.0	0.0	0.003733753505061103	0.0010578968264339792
27	0.0	0.0	0.0	0.0049161087816637855	0.0011201260515183308
28	0.0	0.0	0.0	0.008400945386387481	0.0011201260515183308
29	0.0	0.0	0.0	0.013317054168051267	0.0011201260515183308
30	0.0	0.0	0.0	0.023647105532053653	0.0011201260515183308
31	0.0	0.0	0.0	0.046174085012588975	0.0011201260515183308
32	0.0	0.0	0.0	0.07249704722326974	0.0011201260515183308
33	0.0	0.0	0.0	0.10112249076207154	0.0011201260515183308
34	0.0	0.0	0.0	0.13242379097950044	0.0011823552766026825
35	0.0	0.0	0.0	0.1715037443324733	0.0011823552766026825
36	0.0	0.0	0.0	0.2255187117056906	0.0011823552766026825
37	0.0	0.0	0.0	0.31170618844751774	0.0011823552766026825
38	0.0	0.0	0.0	0.4249011488759535	0.0012445845016870343
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	480	0.0	21.999846	36
TGTAGGA	1630	0.0	20.651445	2
CAATACG	530	0.0	19.924387	38
GTAGGAC	1620	0.0	19.828255	3
CGGGATT	190	0.0	19.684072	44
AATACGA	560	0.0	19.642717	39
CTGTAGG	1660	0.0	19.218525	1
TAGCGGC	575	0.0	19.130299	30
GCAATAC	575	0.0	19.130299	37
CTAGCGG	575	0.0	19.130299	29
TCTAGCG	565	0.0	19.079512	28
GTCCTAA	740	0.0	19.02867	1
CGTCTTA	280	0.0	18.85701	15
ATACGAA	550	0.0	18.799868	40
AGGACCT	1235	0.0	18.170477	5
TAGGACA	645	0.0	18.077953	4
CGAATGC	560	0.0	18.0713	43
GGACCTG	1235	0.0	17.814194	6
TACGAAT	600	0.0	17.599874	41
GCGAGAA	1040	0.0	17.55757	20
>>END_MODULE