FastQCFastQC Report
Thu 2 Feb 2017
SRR4062420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788564
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23160.293698418898149No Hit
TATCAACGCAGAGTACTTTTTTTTT15860.2011250830623767No Hit
GTACATGGAAGCAGTGGTATCAACG14860.18844380418076404No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14080.17855240665310615No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12500.15851598602015818No Hit
TCCATGTACTCTGCGTTGATACCAC12130.1538239128339615No Hit
GCGCAAGACGGACCAGAGCGAAAGC10470.13277298989048447No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9930.12592509929441364No Hit
GATTAAGAGGGACGGCCGGGGGCAT9830.12465697140625238No Hit
GTATCTGATCGTCTTCGAACCTCCG9540.1209794005305847No Hit
GAACTACGACGGTATCTGATCGTCT9290.11780908081018154No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9190.11654095292202028No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9190.11654095292202028No Hit
GAATAGGACCGCGGTTCTATTTTGT9070.11501919945622677No Hit
ATCAGATACCGTCGTAGTTCCGACC8590.10893218559305268No Hit
GAATAACGCCGCCGCATCGCCAGTC8430.10690318097199467No Hit
GTCCTATTCCATTATTCCTAGCTGC8170.10360604846277538No Hit
CTATTGGAGCTGGAATTACCGCGGC8150.10335242288514311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTC250.006044526618.9918123
CGGTCCA1600.014.84583510
CGGTCCC400.005271130714.25200117
CGGGACT400.005282246514.2474774
CAATTAG400.005297841514.2411481
CGCCACT852.6887028E-713.41364817
TAGGACA1102.744855E-912.95225054
CGCCGGT1850.012.8396417
GTTATAG1155.355105E-912.3836071
GCGTTAT701.0959229E-412.2066981
ATCGCCA1251.4024408E-912.16170716
GAATTAA1251.4188117E-912.1524451
CCCTATG550.003078976612.0841652
TCCGTCA1501.4551915E-1112.03502217
AGGACAT1603.6379788E-1211.8751585
TGAGTTA3300.011.80468712
CTGGACT1052.7193346E-711.7598214
TTAATCC658.027633E-411.6902374
ATAGGAG658.0447533E-411.6872693
GAATAAA658.057615E-411.6850431
Produced by FastQC (version 0.11.5)