FastQCFastQC Report
Thu 2 Feb 2017
SRR4062420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788564
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9970.12643235044967815No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9770.12389609467335562No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9710.12313521794045887No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG9310.11806270638781381No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8960.11362425877924937No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8250.10462055077330439No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8200.10398648682922375No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8070.1023379205746141No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7970.10106979268645283No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA7970.10106979268645283No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC7960.10094297989763672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC3650.022.3054851
GTATAAT803.607127E-622.0040591
TTAGCCG1101.2643068E-719.99988632
TCCTACA4850.019.9599132
GCGAGGA3700.019.62150820
ATACACT3950.019.4935594
GTCCTAC5350.019.330671
ACGTGAT1051.7911752E-618.85703537
GGCGTTA3150.018.85703342
ATGCGGC3150.018.85703335
ACTGTTC4250.018.6351878
CGCAATA2600.018.61527636
TTAGGAC3950.018.379643
TAGGACG5050.018.2969254
GGACGTG5200.017.7691276
CGGCGTT3350.017.73124141
GTGTTAC750.001288735717.6032471
GTCCTAA3500.017.6032471
AGGACGT5250.017.5998975
CGACCAT4050.017.38261610
Produced by FastQC (version 0.11.5)