##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062420_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	788564
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046802795968368	32.0	32.0	32.0	32.0	32.0
2	30.98875931439934	32.0	32.0	32.0	32.0	32.0
3	31.244666254102395	32.0	32.0	32.0	32.0	32.0
4	31.442492936527664	32.0	32.0	32.0	32.0	32.0
5	31.287892929426146	32.0	32.0	32.0	32.0	32.0
6	34.87620281930192	36.0	36.0	36.0	32.0	36.0
7	34.88421104691566	36.0	36.0	36.0	32.0	36.0
8	34.80117530092675	36.0	36.0	36.0	32.0	36.0
9	34.98794390816725	36.0	36.0	36.0	36.0	36.0
10	34.79656946043695	36.0	36.0	36.0	32.0	36.0
11	35.01348527196271	36.0	36.0	36.0	36.0	36.0
12	34.87469247898712	36.0	36.0	36.0	32.0	36.0
13	34.944223931095	36.0	36.0	36.0	32.0	36.0
14	34.89245387818871	36.0	36.0	36.0	32.0	36.0
15	34.83138210722275	36.0	36.0	36.0	32.0	36.0
16	34.86905184614058	36.0	36.0	36.0	32.0	36.0
17	34.82489690120269	36.0	36.0	36.0	32.0	36.0
18	34.849613981870846	36.0	36.0	36.0	32.0	36.0
19	34.821432375812236	36.0	36.0	36.0	32.0	36.0
20	34.808922294195526	36.0	36.0	36.0	32.0	36.0
21	34.786437118610536	36.0	36.0	36.0	32.0	36.0
22	34.76840814442455	36.0	36.0	36.0	32.0	36.0
23	34.73674299105716	36.0	36.0	36.0	32.0	36.0
24	34.713876108977836	36.0	36.0	36.0	32.0	36.0
25	34.68164790682811	36.0	36.0	36.0	32.0	36.0
26	34.627483628468966	36.0	36.0	36.0	32.0	36.0
27	34.614480245103756	36.0	36.0	36.0	32.0	36.0
28	34.59262532907919	36.0	36.0	36.0	32.0	36.0
29	34.55812210549809	36.0	36.0	36.0	32.0	36.0
30	34.53991939779143	36.0	36.0	36.0	32.0	36.0
31	34.54534571702487	36.0	36.0	36.0	32.0	36.0
32	34.492616959435125	36.0	36.0	36.0	32.0	36.0
33	34.487335206780934	36.0	36.0	36.0	32.0	36.0
34	34.46052444696943	36.0	36.0	36.0	32.0	36.0
35	34.42936020411786	36.0	36.0	36.0	32.0	36.0
36	34.389699504415624	36.0	36.0	36.0	32.0	36.0
37	34.38832992629641	36.0	36.0	36.0	32.0	36.0
38	34.345238940656685	36.0	36.0	36.0	32.0	36.0
39	34.31826332421972	36.0	36.0	36.0	32.0	36.0
40	34.314141908583196	36.0	36.0	36.0	32.0	36.0
41	34.263160631223336	36.0	36.0	36.0	32.0	36.0
42	34.22709634221192	36.0	36.0	36.0	32.0	36.0
43	34.23159946434278	36.0	36.0	36.0	32.0	36.0
44	34.1726835107867	36.0	36.0	36.0	32.0	36.0
45	34.12061037531513	36.0	36.0	36.0	32.0	36.0
46	34.13447862190006	36.0	36.0	36.0	32.0	36.0
47	34.10622600067972	36.0	36.0	36.0	32.0	36.0
48	34.0508912402798	36.0	36.0	36.0	32.0	36.0
49	34.05312441349085	36.0	36.0	36.0	32.0	36.0
50	33.400303589816424	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	25.0
22	64.0
23	198.0
24	526.0
25	1332.0
26	2732.0
27	5192.0
28	9022.0
29	14727.0
30	22169.0
31	33580.0
32	51724.0
33	87182.0
34	177594.0
35	382493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.166189366133054	18.504759899652743	11.968490555029918	26.360560179184283
2	16.253034080619674	20.985312133025253	36.76735037575678	25.994303410598295
3	18.959368976361976	24.450136958506647	28.678096784011366	27.912397281120015
4	12.843758639143148	16.068235598469112	36.033818520293906	35.05418724209384
5	14.909506393900813	36.27099385718851	32.87634231336962	15.943157435541059
6	33.84929295439933	35.57420269528243	16.707352488006666	13.869151862311572
7	29.68230860145784	30.486174869763268	20.885939505227224	18.94557702355167
8	27.889845199432383	32.96084142928339	19.7495951496583	19.39971822162592
9	27.686148492703193	14.006979775896438	18.620682658604757	39.686189072795614
10	16.34553441445463	26.366915050649027	30.77543484105285	26.51211569384349
11	37.67290923755079	20.893801898133823	22.415808989504974	19.017479874810412
12	24.87293358560624	23.623573990189765	28.636483532091244	22.867008892112754
13	29.751548384151445	19.22646735077939	25.41873582867085	25.603248436398314
14	23.548247193632985	20.18935685626024	24.48995896338154	31.77243698672524
15	25.310564519810697	27.731040219944102	21.989718019082787	24.968677241162418
16	25.718140823065728	25.59259616213776	24.308997113740926	24.38026590105559
17	23.864771914518034	25.80145682531792	25.45538472463871	24.878386535525333
18	25.048315672538944	24.561100937907387	26.839799940144367	23.55078344940931
19	25.94551615341304	24.828295483942963	24.89258956787274	24.333598794771255
20	25.833794086466032	24.448110743072217	24.93253559634982	24.78555957411193
21	27.097863965385184	24.199684489781426	23.63371901329505	25.06873253153834
22	25.834047712043663	25.068098467594258	24.537513759187586	24.56034006117449
23	24.323453771665964	24.550068225280384	25.2665604820915	25.859917520962156
24	24.754744066429613	25.483790789333522	25.047935234172495	24.71352991006437
25	25.025108932185592	24.834509310594953	25.081667435997584	25.058714321221863
26	23.917145596299093	25.8767836218747	25.99522676662896	24.210844015197246
27	25.275944628463893	24.990742666416423	24.979963579367052	24.75334912575263
28	24.088342861200868	25.22027381417361	25.49609162984869	25.195291694776834
29	24.0964588796851	24.959800345945286	25.38462318847931	25.559117585890302
30	24.122709126970037	25.462232615234782	25.650930044993174	24.764128212802007
31	24.932028345194556	25.073424604724536	24.53383618831192	25.460710861768987
32	24.52394479078426	25.61212533161544	24.56744157734819	25.2964883002521
33	24.193090224762987	24.895759887593144	25.641926336987233	25.26922355065664
34	24.774653674273743	25.28317295742641	25.710912494103205	24.231260874196643
35	25.510548287773727	25.16308124641754	25.27226705758822	24.054103408220513
36	23.986004940626252	25.826185319137064	25.115653263400304	25.072156476836376
37	25.294966546786313	25.532486900238915	24.536499256877057	24.636047296097715
38	24.540176827752724	25.259332153128977	24.944075560132088	25.256415458986208
39	24.994927488447356	24.762733272125026	24.699580503294598	25.54275873613302
40	25.63000593483852	24.96791636442952	25.216596243297946	24.18548145743402
41	24.117129364262127	25.101196605475266	26.205735996063733	24.575938034198874
42	25.756184659710563	25.854591383831877	25.03994602847708	23.34927792798048
43	24.263851760922385	24.6948884301084	25.687325315383408	25.35393449358581
44	23.863250161052243	25.405420485845156	25.234984097676282	25.496345255426316
45	24.03482786432046	25.688847068849196	25.34772066693382	24.92860439989652
46	23.869778153144587	24.87959095163094	25.510993428544616	25.739637466679856
47	24.674091132742554	24.616518126620033	25.904048371470168	24.805342369167246
48	24.863295813656215	25.49989601351317	24.57314815284492	25.063660019985694
49	24.054737472164593	25.29927818160606	25.505222150643448	25.140762195585904
50	23.94085958780771	26.115572103215467	24.635540044942452	25.30802826403437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	12.5
2	14.0
3	46.0
4	78.0
5	88.0
6	98.0
7	107.5
8	117.0
9	144.0
10	171.0
11	259.0
12	347.0
13	543.0
14	739.0
15	1020.5
16	1302.0
17	1564.0
18	1826.0
19	2136.0
20	2446.0
21	3025.0
22	3604.0
23	4046.5
24	4489.0
25	5182.5
26	5876.0
27	7705.0
28	9534.0
29	11244.5
30	12955.0
31	14355.0
32	15755.0
33	18385.5
34	21016.0
35	24498.0
36	27980.0
37	31595.5
38	35211.0
39	35922.5
40	36634.0
41	38415.0
42	40196.0
43	39878.0
44	39560.0
45	43390.0
46	47220.0
47	50690.5
48	54161.0
49	55196.5
50	56232.0
51	52982.0
52	49732.0
53	48900.5
54	48069.0
55	49805.5
56	51542.0
57	50904.0
58	50266.0
59	45804.5
60	41343.0
61	36723.0
62	32103.0
63	29214.5
64	26326.0
65	22794.5
66	19263.0
67	17596.5
68	15930.0
69	14594.0
70	13258.0
71	10437.0
72	7616.0
73	6694.0
74	5772.0
75	4441.5
76	3111.0
77	2735.5
78	2360.0
79	2034.5
80	1709.0
81	1370.5
82	1032.0
83	896.0
84	760.0
85	597.5
86	435.0
87	330.5
88	226.0
89	156.0
90	86.0
91	56.0
92	26.0
93	19.5
94	13.0
95	9.0
96	5.0
97	4.5
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012934904459244905
2	0.0027898813539547836
3	5.072511552645061E-4
4	2.5362557763225306E-4
5	0.0
6	1.2681278881612653E-4
7	0.0
8	1.2681278881612653E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.5362557763225306E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	788564.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.28397551867247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98225318367922	44.77020902453458
2	11.477067720467142	12.689958617689031
3	3.2710556158250754	5.425108757564687
4	1.3416768063813842	2.9669291087183645
5	0.7087571038514292	1.9591455189003806
6	0.43289127487786744	1.4359170385556963
7	0.2896727940771083	1.1209984559329014
8	0.21258031555318455	0.9401827968635139
9	0.15030363451112225	0.7478444205612339
>10	0.9109483578139933	9.656464094040402
>50	0.10978584495500732	4.279110390320069
>100	0.1058724645291346	11.25974273485222
>500	0.007134883478317426	2.7483890414670435
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	997	0.12643235044967815	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	977	0.12389609467335562	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	971	0.12313521794045887	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	931	0.11806270638781381	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	896	0.11362425877924937	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	825	0.10462055077330439	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	820	0.10398648682922375	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	807	0.1023379205746141	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	797	0.10106979268645283	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	797	0.10106979268645283	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	796	0.10094297989763672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.2681278881612653E-4
9	0.0	0.0	0.0	0.0	1.2681278881612653E-4
10	0.0	0.0	0.0	0.0	1.2681278881612653E-4
11	0.0	0.0	0.0	0.0	1.2681278881612653E-4
12	0.0	0.0	0.0	0.0	1.2681278881612653E-4
13	0.0	0.0	0.0	0.0	1.2681278881612653E-4
14	0.0	0.0	0.0	0.0	1.2681278881612653E-4
15	0.0	0.0	0.0	1.2681278881612653E-4	1.2681278881612653E-4
16	0.0	0.0	0.0	2.5362557763225306E-4	2.5362557763225306E-4
17	0.0	0.0	0.0	3.804383664483796E-4	2.5362557763225306E-4
18	0.0	0.0	0.0	3.804383664483796E-4	2.5362557763225306E-4
19	0.0	0.0	0.0	3.804383664483796E-4	2.5362557763225306E-4
20	0.0	0.0	0.0	3.804383664483796E-4	5.072511552645061E-4
21	0.0	0.0	0.0	5.072511552645061E-4	5.072511552645061E-4
22	0.0	0.0	0.0	0.0010145023105290123	5.072511552645061E-4
23	0.0	0.0	0.0	0.001902191832241898	5.072511552645061E-4
24	0.0	0.0	0.0	0.004058009242116049	5.072511552645061E-4
25	0.0	0.0	0.0	0.004438447608564429	5.072511552645061E-4
26	0.0	0.0	0.0	0.006340639440806327	5.072511552645061E-4
27	0.0	0.0	0.0	0.007735580117783718	5.072511552645061E-4
28	0.0	0.0	0.0	0.013315342825693285	5.072511552645061E-4
29	0.0	0.0	0.0	0.023967617086247915	5.072511552645061E-4
30	0.0	0.0	0.0	0.04438447608564428	5.072511552645061E-4
31	0.0	0.0	0.0	0.09282696141340462	5.072511552645061E-4
32	0.0	0.0	0.0	0.15039996753592608	6.340639440806326E-4
33	0.0	0.0	0.0	0.2098751654906894	6.340639440806326E-4
34	0.0	0.0	0.0	0.28050988886127187	6.340639440806326E-4
35	0.0	0.0	0.0	0.3619236992812251	6.340639440806326E-4
36	0.0	0.0	0.0	0.47427983017231323	6.340639440806326E-4
37	0.0	0.0	0.0	0.634444382447081	6.340639440806326E-4
38	0.0	0.0	0.0	0.8472362420805414	6.340639440806326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	365	0.0	22.305485	1
GTATAAT	80	3.607127E-6	22.004059	1
TTAGCCG	110	1.2643068E-7	19.999886	32
TCCTACA	485	0.0	19.959913	2
GCGAGGA	370	0.0	19.621508	20
ATACACT	395	0.0	19.493559	4
GTCCTAC	535	0.0	19.33067	1
ACGTGAT	105	1.7911752E-6	18.857035	37
GGCGTTA	315	0.0	18.857033	42
ATGCGGC	315	0.0	18.857033	35
ACTGTTC	425	0.0	18.635187	8
CGCAATA	260	0.0	18.615276	36
TTAGGAC	395	0.0	18.37964	3
TAGGACG	505	0.0	18.296925	4
GGACGTG	520	0.0	17.769127	6
CGGCGTT	335	0.0	17.731241	41
GTGTTAC	75	0.0012887357	17.603247	1
GTCCTAA	350	0.0	17.603247	1
AGGACGT	525	0.0	17.599897	5
CGACCAT	405	0.0	17.382616	10
>>END_MODULE