FastQCFastQC Report
Thu 2 Feb 2017
SRR4062419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689354
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG60160.35611245482000814No Hit
TCCATGTACTCTGCGTTGATACCAC54190.3207735027708816No Hit
GTATCAACGCAGAGTACTTTTTTTT43560.2578500420870936No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31210.1847451747827868No Hit
TATCAACGCAGAGTACTTTTTTTTT29590.17515571040764694No Hit
GAGTACATGGAAGCAGTGGTATCAA26590.15739744304627687No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23220.13744898937700448No Hit
CATGTACTCTGCGTTGATACCACTG22350.13229909184220714No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18190.10767429443444061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCTA307.7217235E-419.00050514
ATACGAT350.002169489616.2866296
TCGTGCG456.7598745E-414.7786079
TCGCGTA852.697634E-713.4125189
AATTTCG801.9911258E-613.06749418
AAGACGG3300.012.9525855
TTAATAC1251.03682396E-1012.9146013
CGAACGA751.4747571E-512.67000716
TTCGCAG604.0893856E-412.66775415
CGCGTAA905.4021984E-712.66737810
CAAGACG3500.012.4834554
CGCCAGT2450.012.41290918
CGCAGTT550.003060537612.09517417
CGAACGT1650.012.088724
TAATACC951.0422118E-611.99782854
TCCAACG1750.011.94742318
CGCAAGA3100.011.9452022
CGGTCCA2250.011.82288610
GCTCGTA1052.7182068E-711.7625679
TCGAACG1701.8189894E-1211.730393
Produced by FastQC (version 0.11.5)