FastQCFastQC Report
Thu 2 Feb 2017
SRR4062419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689354
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29800.17639878912294285No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26440.15650952967820836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT500.002580999421.9997944
TCGTATA602.873615E-421.99979244
GGCGTTA4000.019.79981442
GTATTAG5250.019.7003021
TTAACGG2600.019.46135535
TAACGGC2700.018.74056436
AATCACG4950.018.2220534
TCACGGA5100.018.11747636
TAGGACG7050.017.7870674
ATCACGG4950.017.7776135
TGTAGGA9700.017.465822
GATATAC7450.017.4272981
CAATACG4550.017.4064338
AGGACGT7250.017.2963895
GCATTCG5000.017.15983822
TACCGTC3850.017.1426967
ACCGGAC902.2161867E-417.110958
GTCGTAT902.2161867E-417.1109543
GGACGTG7350.017.0610646
CGGCGTT4950.016.8887341
Produced by FastQC (version 0.11.5)