##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062419_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1689354
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225323407645764	32.0	32.0	32.0	32.0	32.0
2	31.365819715701978	32.0	32.0	32.0	32.0	32.0
3	31.47791167511368	32.0	32.0	32.0	32.0	32.0
4	31.594985420462496	32.0	32.0	32.0	32.0	32.0
5	31.518424202387422	32.0	32.0	32.0	32.0	32.0
6	35.11015926798054	36.0	36.0	36.0	36.0	36.0
7	35.12497913403585	36.0	36.0	36.0	36.0	36.0
8	35.06130449864268	36.0	36.0	36.0	36.0	36.0
9	35.19040059099514	36.0	36.0	36.0	36.0	36.0
10	35.07212934648392	36.0	36.0	36.0	36.0	36.0
11	35.21159804280216	36.0	36.0	36.0	36.0	36.0
12	35.11349663835999	36.0	36.0	36.0	36.0	36.0
13	35.16516313336341	36.0	36.0	36.0	36.0	36.0
14	35.123309264961634	36.0	36.0	36.0	36.0	36.0
15	35.081329312861605	36.0	36.0	36.0	36.0	36.0
16	35.09664759428752	36.0	36.0	36.0	36.0	36.0
17	35.07184817391737	36.0	36.0	36.0	36.0	36.0
18	35.08629985189605	36.0	36.0	36.0	36.0	36.0
19	35.0638054546294	36.0	36.0	36.0	36.0	36.0
20	35.04883345941703	36.0	36.0	36.0	36.0	36.0
21	35.03392657785165	36.0	36.0	36.0	36.0	36.0
22	35.010506974855474	36.0	36.0	36.0	36.0	36.0
23	34.96685537785449	36.0	36.0	36.0	36.0	36.0
24	34.95121626373158	36.0	36.0	36.0	36.0	36.0
25	34.92106864517443	36.0	36.0	36.0	32.0	36.0
26	34.87695355739531	36.0	36.0	36.0	32.0	36.0
27	34.86085036055202	36.0	36.0	36.0	32.0	36.0
28	34.8385637350135	36.0	36.0	36.0	32.0	36.0
29	34.80728728259441	36.0	36.0	36.0	32.0	36.0
30	34.79157535957532	36.0	36.0	36.0	32.0	36.0
31	34.78820365654564	36.0	36.0	36.0	32.0	36.0
32	34.741745069417064	36.0	36.0	36.0	32.0	36.0
33	34.71322292426572	36.0	36.0	36.0	32.0	36.0
34	34.700225056441695	36.0	36.0	36.0	32.0	36.0
35	34.65397720075248	36.0	36.0	36.0	32.0	36.0
36	34.61798000892649	36.0	36.0	36.0	32.0	36.0
37	34.590886220413246	36.0	36.0	36.0	32.0	36.0
38	34.55106863333558	36.0	36.0	36.0	32.0	36.0
39	34.506160934889905	36.0	36.0	36.0	32.0	36.0
40	34.48463258736771	36.0	36.0	36.0	32.0	36.0
41	34.45695810351176	36.0	36.0	36.0	32.0	36.0
42	34.3980983263425	36.0	36.0	36.0	32.0	36.0
43	34.39872223346912	36.0	36.0	36.0	32.0	36.0
44	34.361589104474255	36.0	36.0	36.0	32.0	36.0
45	34.278143006143175	36.0	36.0	36.0	32.0	36.0
46	34.30081853773691	36.0	36.0	36.0	32.0	36.0
47	34.25015124124369	36.0	36.0	36.0	32.0	36.0
48	34.22167526758749	36.0	36.0	36.0	32.0	36.0
49	34.20879815598152	36.0	36.0	36.0	32.0	36.0
50	33.66363533042808	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	62.0
22	156.0
23	448.0
24	1243.0
25	2923.0
26	6006.0
27	11166.0
28	18845.0
29	28124.0
30	40839.0
31	58453.0
32	86466.0
33	137912.0
34	298096.0
35	998601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04480545622469	18.245257773462477	11.828154971937387	25.881781798375446
2	15.495422326355632	21.010059871448647	37.74813737875055	25.74638042344517
3	18.59979033318791	25.153861225668855	28.829719412293624	27.41662902884961
4	11.958542708362314	16.248350699942524	36.903122082832894	34.88998450886227
5	13.686651820755152	37.59028599097643	33.967836226155086	14.755225962113327
6	33.31186547228012	36.33433510107752	17.147276432846535	13.206522993795828
7	29.068211872704	31.00303429594981	21.458616725683306	18.470137105662875
8	27.499091368653346	33.7804273112681	20.06506629161206	18.6554150284665
9	27.25083079094139	14.354481061991745	18.76350368247271	39.63118446459416
10	15.284895883278459	27.610968453029976	32.57132608085694	24.532809582834624
11	37.12277000557609	21.26380853272908	22.62740668918415	18.986014772510675
12	23.9289692983235	24.097199284460213	29.9383077791866	22.035523638029687
13	29.686377159553295	20.079450488174768	25.674784562619795	24.55938778965214
14	23.058340643820063	20.299120255434918	25.76020182862798	30.882337272117034
15	24.8198423776189	27.98768049798917	23.342591310051063	23.849885814340865
16	25.065320826777572	26.172548796759	25.104270626523512	23.65785974993992
17	23.112207388149553	26.322724544411653	26.437028591994338	24.12803947544446
18	24.098619945849123	25.25219699364372	27.838451857929126	22.810731202578026
19	24.844585563475743	25.948439462658506	25.865626742529983	23.341348231335765
20	24.75697811115965	25.06247950399975	26.542808671243563	23.63773371359703
21	25.962350105424914	24.98090986258653	24.81303504179704	24.243704990191517
22	24.492439121699775	25.62156895476022	25.784708237586674	24.10128368595333
23	23.645843322358722	25.1525139195219	26.058836691421693	25.142806066697688
24	23.853023108241374	26.189123179629608	25.97288667739266	23.984967034736353
25	24.456981781201574	25.267942657370806	26.033442369094935	24.24163319233269
26	23.29434801705267	26.042499085449233	26.72802739982265	23.935125497675443
27	24.512920323389885	25.80299925296889	26.015151353712724	23.668929069928506
28	23.533670266859403	25.505370691992326	26.353031987375058	24.607927053773217
29	23.503717989243224	25.66436637910112	26.464731489078076	24.367184142577578
30	23.908369708184313	25.752269802539907	26.58483657066547	23.75452391861031
31	23.892209684885465	25.585993225812942	25.884924059729343	24.63687302957225
32	23.60251314999698	26.07097150745196	25.87811672390748	24.448398618643576
33	23.465656102865356	25.46908463235059	26.389436435465864	24.67582282931819
34	23.745052842684245	26.025095983435087	26.81894972871287	23.4109014451678
35	24.791369955616172	25.651876397723626	26.274481251413263	23.28227239524694
36	23.573152814626184	26.28803672883244	26.011481311791375	24.127329144750004
37	24.55832229361046	25.879478191071854	25.80737962558469	23.754819889732996
38	23.827155232118315	25.901853607947178	25.873026020597223	24.397965139337284
39	24.204104053975662	25.499688046436685	25.90978563403526	24.386422265552394
40	24.955574734484305	25.67537650486517	25.931154749093437	23.43789401155708
41	23.42374659189252	25.70189551745815	26.928695821006137	23.945662069643188
42	25.029745097830297	26.317929812224083	25.947669937739516	22.70465515220611
43	23.857225898183565	25.219817752821495	26.31573962591618	24.60721672307876
44	23.430139568142614	26.014736994140957	26.309761009237853	24.24536242847858
45	23.6701721486438	26.123535978841616	26.202737851273326	24.003554021241253
46	23.482139897428127	25.54293007022811	26.353655129304016	24.621274903039744
47	24.130007168424836	25.365332171547333	26.583372450873206	23.92128820915463
48	24.180663141058652	26.51480980303714	25.280787804095528	24.023739251808678
49	23.40148956346627	26.03930259732418	26.421342122491794	24.13786571671775
50	23.318513063877116	27.03958260943687	25.21657699722912	24.4253273294569
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	59.0
2	57.0
3	219.0
4	381.0
5	404.0
6	427.0
7	388.5
8	350.0
9	446.0
10	542.0
11	832.0
12	1122.0
13	1912.5
14	2703.0
15	3617.5
16	4532.0
17	5263.5
18	5995.0
19	6668.5
20	7342.0
21	8649.5
22	9957.0
23	10831.5
24	11706.0
25	13836.0
26	15966.0
27	20325.5
28	24685.0
29	28047.5
30	31410.0
31	34290.0
32	37170.0
33	42456.0
34	47742.0
35	54461.5
36	61181.0
37	68811.0
38	76441.0
39	79571.0
40	82701.0
41	87561.0
42	92421.0
43	93345.5
44	94270.0
45	105101.0
46	115932.0
47	123010.5
48	130089.0
49	128930.0
50	127771.0
51	119507.5
52	111244.0
53	107256.0
54	103268.0
55	103665.0
56	104062.0
57	100865.5
58	97669.0
59	88212.0
60	78755.0
61	69649.5
62	60544.0
63	52330.5
64	44117.0
65	38117.0
66	32117.0
67	27771.5
68	23426.0
69	21406.0
70	19386.0
71	15027.5
72	10669.0
73	9689.5
74	8710.0
75	6290.5
76	3871.0
77	3461.0
78	3051.0
79	2595.0
80	2139.0
81	1770.5
82	1402.0
83	1162.0
84	922.0
85	766.5
86	611.0
87	450.0
88	289.0
89	190.0
90	91.0
91	62.0
92	33.0
93	20.5
94	8.0
95	6.5
96	5.0
97	6.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01621921752338468
2	0.00402520726857722
3	4.14359571765302E-4
4	5.919422453790028E-5
5	0.0
6	4.14359571765302E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1838844907580056E-4
47	5.919422453790028E-5
48	0.0
49	0.0
50	5.919422453790028E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1689354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.13743686525074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23504095750619	42.585421945795524
2	14.013023413481044	15.45284387504183
3	4.143456708970305	6.853787480842495
4	1.7244515408830108	3.803273518504855
5	0.8397148516655973	2.3149862309262637
6	0.49362490505910533	1.6330327222687087
7	0.3033020112365641	1.1706306846961738
8	0.19842287169621747	0.8752422848617553
9	0.14985048753939798	0.7436134616337539
>10	0.7323716329799308	7.428741397089922
>50	0.07804337843914412	3.0491655027668743
>100	0.07736762903308958	8.957021342588819
>500	0.008847887084347882	3.2206416966462057
>1k	0.0024817244260975763	1.9115978563368534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2980	0.17639878912294285	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2644	0.15650952967820836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3677689815160114E-4
2	0.0	0.0	0.0	0.0	2.3677689815160114E-4
3	0.0	0.0	0.0	5.9194224537900286E-5	2.3677689815160114E-4
4	0.0	0.0	0.0	1.1838844907580057E-4	2.3677689815160114E-4
5	0.0	0.0	0.0	1.1838844907580057E-4	2.3677689815160114E-4
6	0.0	0.0	0.0	1.1838844907580057E-4	2.959711226895014E-4
7	0.0	0.0	0.0	1.1838844907580057E-4	2.959711226895014E-4
8	0.0	0.0	0.0	1.1838844907580057E-4	2.959711226895014E-4
9	0.0	0.0	0.0	1.7758267361370087E-4	2.959711226895014E-4
10	0.0	0.0	0.0	1.7758267361370087E-4	2.959711226895014E-4
11	0.0	0.0	0.0	1.7758267361370087E-4	3.5516534722740173E-4
12	0.0	0.0	0.0	1.7758267361370087E-4	4.735537963032023E-4
13	0.0	0.0	0.0	2.3677689815160114E-4	8.28719143530604E-4
14	0.0	0.0	0.0	2.959711226895014E-4	8.28719143530604E-4
15	0.0	0.0	0.0	2.959711226895014E-4	9.471075926064046E-4
16	0.0	0.0	0.0	3.5516534722740173E-4	0.001006301817144305
17	0.0	0.0	0.0	3.5516534722740173E-4	0.001006301817144305
18	0.0	0.0	0.0	7.103306944548035E-4	0.001006301817144305
19	0.0	0.0	0.0	7.103306944548035E-4	0.0010654960416822052
20	0.0	0.0	0.0	9.471075926064046E-4	0.0011838844907580057
21	0.0	0.0	0.0	0.0013614671643717067	0.0011838844907580057
22	0.0	0.0	0.0	0.002249380532440211	0.0011838844907580057
23	0.0	0.0	0.0	0.003966013044039319	0.0011838844907580057
24	0.0	0.0	0.0	0.006688947372782732	0.0011838844907580057
25	0.0	0.0	0.0	0.008583162557995542	0.0011838844907580057
26	0.0	0.0	0.0	0.01041818351867045	0.0013022729398338062
27	0.0	0.0	0.0	0.012785952500186461	0.0013022729398338062
28	0.0	0.0	0.0	0.019001346076665992	0.0013022729398338062
29	0.0	0.0	0.0	0.029893083391639645	0.0013022729398338062
30	0.0	0.0	0.0	0.05084783887805635	0.0013022729398338062
31	0.0	0.0	0.0	0.08790342343878192	0.0013614671643717067
32	0.0	0.0	0.0	0.13413411280288204	0.0013614671643717067
33	0.0	0.0	0.0	0.17929930612529996	0.0013614671643717067
34	0.0	0.0	0.0	0.23139022371865223	0.0014798556134475072
35	0.0	0.0	0.0	0.2907028367056283	0.0014798556134475072
36	0.0	0.0	0.0	0.37363394528322663	0.0014798556134475072
37	0.0	0.0	0.0	0.502854937449463	0.0014798556134475072
38	0.0	0.0	0.0	0.669072319951887	0.0014798556134475072
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGGT	50	0.0025809994	21.999794	4
TCGTATA	60	2.873615E-4	21.999792	44
GGCGTTA	400	0.0	19.799814	42
GTATTAG	525	0.0	19.700302	1
TTAACGG	260	0.0	19.461355	35
TAACGGC	270	0.0	18.740564	36
AATCACG	495	0.0	18.22205	34
TCACGGA	510	0.0	18.117476	36
TAGGACG	705	0.0	17.787067	4
ATCACGG	495	0.0	17.77761	35
TGTAGGA	970	0.0	17.46582	2
GATATAC	745	0.0	17.427298	1
CAATACG	455	0.0	17.40643	38
AGGACGT	725	0.0	17.296389	5
GCATTCG	500	0.0	17.159838	22
TACCGTC	385	0.0	17.142696	7
ACCGGAC	90	2.2161867E-4	17.11095	8
GTCGTAT	90	2.2161867E-4	17.11095	43
GGACGTG	735	0.0	17.061064	6
CGGCGTT	495	0.0	16.88873	41
>>END_MODULE