FastQCFastQC Report
Thu 2 Feb 2017
SRR4062418_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062418_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614472
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36880.22843381613307634No Hit
GTACATGGAAGCAGTGGTATCAACG25520.1580702545476168No Hit
TATCAACGCAGAGTACTTTTTTTTT24150.14958450812401825No Hit
TCCATGTACTCTGCGTTGATACCAC21510.1332324128259889No Hit
GCGCAAGACGGACCAGAGCGAAAGC19410.1202250642934656No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18600.11520794414520659No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18480.11446466708620529No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16680.10331551120118528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT456.7609345E-414.7782356
AAGACGG3400.013.4113495
GACTGCG500.001499239113.3004127
CAAGACG3550.012.8434774
CGAACGA1207.385097E-1012.66941716
AATCGTA604.0921732E-412.66666713
AACCGCG1700.012.2944997
CGCGTAA1405.2750693E-1112.21466410
GTATTAG2650.011.8221231
ACGAACG1302.6066118E-911.69448415
TCGCGTA1501.7644197E-1011.4003549
CGGTCCA3100.011.33906110
CGCAAGA3700.011.2923952
GCAAGAC4600.011.1490623
ACGGTAT2900.011.1382769
CGCGCTA1201.2712917E-711.0857416
ACGCGCG1201.2751116E-711.08333413
ACTAGGC600.005888038311.080243
GAGTAAT600.005905725511.0757761
TACGCTA2150.011.0468559
Produced by FastQC (version 0.11.5)