##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062418_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1614472
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205035454315713	32.0	32.0	32.0	32.0	32.0
2	30.876288966299818	32.0	32.0	32.0	32.0	32.0
3	30.86015861532439	32.0	32.0	32.0	32.0	32.0
4	30.926058178772998	32.0	32.0	32.0	32.0	32.0
5	30.736599953421305	32.0	32.0	32.0	32.0	32.0
6	34.3901108226095	36.0	36.0	36.0	32.0	36.0
7	34.28071902145098	36.0	36.0	36.0	32.0	36.0
8	34.28871358561808	36.0	36.0	36.0	32.0	36.0
9	34.44043129890144	36.0	36.0	36.0	32.0	36.0
10	34.11963601722421	36.0	36.0	36.0	32.0	36.0
11	34.421900163025434	36.0	36.0	36.0	32.0	36.0
12	34.202725720854865	36.0	36.0	36.0	32.0	36.0
13	34.32695085452086	36.0	36.0	36.0	32.0	36.0
14	34.230513133705635	36.0	36.0	36.0	32.0	36.0
15	34.17186609616023	36.0	36.0	36.0	32.0	36.0
16	34.17139597342041	36.0	36.0	36.0	32.0	36.0
17	34.09484958549916	36.0	36.0	36.0	32.0	36.0
18	34.10986935666893	36.0	36.0	36.0	32.0	36.0
19	34.10358742672527	36.0	36.0	36.0	32.0	36.0
20	34.096179432037225	36.0	36.0	36.0	32.0	36.0
21	34.08140246470673	36.0	36.0	36.0	32.0	36.0
22	34.035884797011036	36.0	36.0	36.0	32.0	36.0
23	34.006182826335795	36.0	36.0	36.0	32.0	36.0
24	33.97476450505181	36.0	36.0	36.0	32.0	36.0
25	33.53183084005173	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	58.0
5	141.0
6	248.0
7	74.0
8	258.0
9	289.0
10	210.0
11	64.0
12	122.0
13	95.0
14	268.0
15	334.0
16	513.0
17	685.0
18	939.0
19	1259.0
20	1939.0
21	2829.0
22	4355.0
23	6495.0
24	9223.0
25	13102.0
26	17968.0
27	24061.0
28	32298.0
29	42689.0
30	55682.0
31	76632.0
32	109901.0
33	159279.0
34	360610.0
35	691841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79447067809178	17.10724946866798	11.158003368561374	25.94027648467887
2	17.039238507713968	19.58425652978892	36.69119783152844	26.68530713096867
3	19.491918604399068	22.953108517350547	28.10565248605198	29.449320392198402
4	12.917204333715146	14.629788747183635	35.204961879586946	37.248045039514274
5	15.028244526906498	35.98043546821104	33.20858693099686	15.782733073885598
6	35.214111100439034	34.393793396188414	16.40226427020043	13.989831233172117
7	30.89500332524902	30.059011548809885	20.213299384596503	18.83268574134459
8	28.443499656863562	32.51185169066762	19.29697636977151	19.747672282697305
9	27.061084675356884	14.52268394376676	18.688965698963063	39.727265681913295
10	16.16611992037555	26.352840496846646	31.16639897802886	26.31464060474894
11	37.54198293396835	21.127958029468672	21.946842288038894	19.383216748524084
12	24.855955592768318	23.434676093900208	28.22606754116662	23.483300772164853
13	29.306195245646162	19.15979914954206	25.40403573734158	26.129969867470198
14	23.510755798990253	19.51870380947597	24.743329990654143	32.22721040087964
15	25.166650750874027	27.150275260533256	22.265470460495557	25.41760352809716
16	25.940155703066626	25.75295237445626	23.640574569739286	24.666317352737828
17	24.038477042395197	25.884685462395517	25.267752934274483	24.8090845609348
18	25.037676988817843	24.55466729513331	26.32390426633755	24.0837514497113
19	25.41488924916179	25.136787239558462	25.262937883522145	24.185385627757608
20	25.581952044770123	24.21643605225165	25.238324990201576	24.96328691277665
21	26.345858543761334	24.229609011999294	24.558429895784496	24.866102548454876
22	25.531064908898266	24.43872499758071	24.984863936716366	25.045346156804655
23	24.12546619370709	24.286110358199274	25.54583351682329	26.04258993127035
24	24.597681758782095	25.037662581721136	25.355895116609805	25.008760542886964
25	24.87352492258305	24.427040145177326	25.300101155253778	25.399333776985845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	174.0
1	174.0
2	452.0
3	730.0
4	730.0
5	730.0
6	1987.0
7	3244.0
8	3244.0
9	3244.0
10	3480.0
11	3716.0
12	3716.0
13	3716.0
14	3992.5
15	4269.0
16	4269.0
17	4269.0
18	7065.0
19	9861.0
20	9861.0
21	9861.0
22	15821.5
23	21782.0
24	21782.0
25	21782.0
26	32147.5
27	42513.0
28	42513.0
29	42513.0
30	53713.0
31	64913.0
32	64913.0
33	64913.0
34	80514.5
35	96116.0
36	96116.0
37	96116.0
38	110861.0
39	125606.0
40	125606.0
41	125606.0
42	145204.0
43	164802.0
44	164802.0
45	164802.0
46	190211.5
47	215621.0
48	215621.0
49	215621.0
50	218301.5
51	220982.0
52	220982.0
53	220982.0
54	205447.0
55	189912.0
56	189912.0
57	189912.0
58	174989.5
59	160067.0
60	160067.0
61	160067.0
62	141368.5
63	122670.0
64	122670.0
65	122670.0
66	100982.5
67	79295.0
68	79295.0
69	79295.0
70	60262.0
71	41229.0
72	41229.0
73	41229.0
74	31903.0
75	22577.0
76	22577.0
77	22577.0
78	18426.5
79	14276.0
80	14276.0
81	14276.0
82	9994.0
83	5712.0
84	5712.0
85	5712.0
86	4169.5
87	2627.0
88	2627.0
89	2627.0
90	1775.0
91	923.0
92	923.0
93	923.0
94	543.0
95	163.0
96	163.0
97	163.0
98	427.5
99	692.0
100	692.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00823798740393144
2	0.0039022045597569975
3	0.005264879167926108
4	0.015175239954610547
5	0.032890009860808984
6	0.043791406726161865
7	0.06559420045686763
8	0.0873969941875734
9	0.09582080085625518
10	0.11780941385171127
11	0.11842881140087903
12	0.13143615993340238
13	0.11948178723446426
14	0.12344593154913805
15	0.11260647443870195
16	0.11068634203628183
17	0.1225168352253864
18	0.12895856973673128
19	0.13094064189406815
20	0.12189743767621859
21	0.12660485904989371
22	0.15038972493793637
23	0.13707267763082917
24	0.1318697382178198
25	0.13001154557031647
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1614472.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.3220242579928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65121078249044	39.578881369169885
2	12.22434741409847	12.303078142207895
3	3.594588328247101	5.426608831546452
4	1.5827193381554718	3.1858256371301583
5	0.8590264672119763	2.1613975360649467
6	0.581999637874592	1.7572439937160944
7	0.40781091852852003	1.4365309654406557
8	0.2874879248774092	1.1573579463648798
9	0.22856246732814384	1.0351553422818187
>10	1.3769965109328215	12.525221448991472
>50	0.10157205513114734	3.5250211276523467
>100	0.08948426512426018	9.537114599904168
>500	0.010244285825907234	3.395244466156136
>1k	0.003949604173843753	2.975318593373155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3688	0.22843381613307634	No Hit
GTACATGGAAGCAGTGGTATCAACG	2552	0.1580702545476168	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2415	0.14958450812401825	No Hit
TCCATGTACTCTGCGTTGATACCAC	2151	0.1332324128259889	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1941	0.1202250642934656	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1860	0.11520794414520659	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1848	0.11446466708620529	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1668	0.10331551120118528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.193975491677775E-5
2	0.0	0.0	0.0	0.0	6.193975491677775E-5
3	0.0	0.0	0.0	0.0	6.193975491677775E-5
4	0.0	0.0	0.0	0.0	6.193975491677775E-5
5	0.0	0.0	0.0	6.193975491677775E-5	6.193975491677775E-5
6	0.0	0.0	0.0	6.193975491677775E-5	6.193975491677775E-5
7	0.0	0.0	0.0	1.8581926475033325E-4	6.193975491677775E-5
8	0.0	0.0	0.0	1.8581926475033325E-4	6.193975491677775E-5
9	0.0	0.0	0.0	2.47759019667111E-4	6.193975491677775E-5
10	0.0	0.0	0.0	2.47759019667111E-4	6.193975491677775E-5
11	0.0	0.0	0.0	3.0969877458388874E-4	6.193975491677775E-5
12	0.0	0.0	0.0	3.0969877458388874E-4	2.47759019667111E-4
13	0.0	0.0	0.0	3.0969877458388874E-4	2.47759019667111E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	45	6.7609345E-4	14.778235	6
AAGACGG	340	0.0	13.411349	5
GACTGCG	50	0.0014992391	13.300412	7
CAAGACG	355	0.0	12.843477	4
CGAACGA	120	7.385097E-10	12.669417	16
AATCGTA	60	4.0921732E-4	12.666667	13
AACCGCG	170	0.0	12.294499	7
CGCGTAA	140	5.2750693E-11	12.214664	10
GTATTAG	265	0.0	11.822123	1
ACGAACG	130	2.6066118E-9	11.694484	15
TCGCGTA	150	1.7644197E-10	11.400354	9
CGGTCCA	310	0.0	11.339061	10
CGCAAGA	370	0.0	11.292395	2
GCAAGAC	460	0.0	11.149062	3
ACGGTAT	290	0.0	11.138276	9
CGCGCTA	120	1.2712917E-7	11.08574	16
ACGCGCG	120	1.2751116E-7	11.083334	13
ACTAGGC	60	0.0058880383	11.08024	3
GAGTAAT	60	0.0059057255	11.075776	1
TACGCTA	215	0.0	11.046855	9
>>END_MODULE