FastQCFastQC Report
Thu 2 Feb 2017
SRR4062418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614472
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18250.11304005272311937No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17730.10981918546744693No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16940.1049259448290215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC602.8675335E-422.0066011
TAGGACG3000.019.7998074
CCTAATA1152.020679E-719.1320232
CGTCGTA3150.018.85695810
GTATAGG1557.185008E-1018.4571481
CTAGATA3700.018.4328233
TATACCG851.4313172E-418.1174685
GTCGATA1254.855465E-717.59982736
TTAGCCG1154.2750817E-617.2172232
ACCGTCG3450.017.217228
TCTAGAT4000.017.0514162
TACCGTC3750.017.0131657
TTAGGAC2500.016.7203523
TGGCGAG4300.016.37193118
CCGTCGT3500.016.3426979
TTGCGCG3950.016.1517418
CGCCCCT3150.016.0633355
CGTCTAT1105.6087134E-516.0047991
TATGGCG4400.015.99984216
ATACCGT4000.015.9498426
Produced by FastQC (version 0.11.5)