##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1614472
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313093692550876	32.0	32.0	32.0	32.0	32.0
2	31.458386395056714	32.0	32.0	32.0	32.0	32.0
3	31.526565341486258	32.0	32.0	32.0	32.0	32.0
4	31.61917952123047	32.0	32.0	32.0	32.0	32.0
5	31.56574099767602	32.0	32.0	32.0	32.0	32.0
6	35.16929064115079	36.0	36.0	36.0	36.0	36.0
7	35.182411339434815	36.0	36.0	36.0	36.0	36.0
8	35.11987200769044	36.0	36.0	36.0	36.0	36.0
9	35.23995832693289	36.0	36.0	36.0	36.0	36.0
10	35.12989757642127	36.0	36.0	36.0	36.0	36.0
11	35.24660322384037	36.0	36.0	36.0	36.0	36.0
12	35.160152669107916	36.0	36.0	36.0	36.0	36.0
13	35.20484591866567	36.0	36.0	36.0	36.0	36.0
14	35.16877096660704	36.0	36.0	36.0	36.0	36.0
15	35.136709091269466	36.0	36.0	36.0	36.0	36.0
16	35.14567672898632	36.0	36.0	36.0	36.0	36.0
17	35.11740246966191	36.0	36.0	36.0	36.0	36.0
18	35.13105832742841	36.0	36.0	36.0	36.0	36.0
19	35.118482698987656	36.0	36.0	36.0	36.0	36.0
20	35.10528829239529	36.0	36.0	36.0	36.0	36.0
21	35.097929849511175	36.0	36.0	36.0	36.0	36.0
22	35.082986883637496	36.0	36.0	36.0	36.0	36.0
23	35.04351081963639	36.0	36.0	36.0	36.0	36.0
24	35.027252872765835	36.0	36.0	36.0	36.0	36.0
25	35.00099041668112	36.0	36.0	36.0	36.0	36.0
26	34.95981039002225	36.0	36.0	36.0	36.0	36.0
27	34.94270448790688	36.0	36.0	36.0	32.0	36.0
28	34.9276240157773	36.0	36.0	36.0	32.0	36.0
29	34.90742236471119	36.0	36.0	36.0	32.0	36.0
30	34.895524357189224	36.0	36.0	36.0	32.0	36.0
31	34.889599819631435	36.0	36.0	36.0	32.0	36.0
32	34.861370776328116	36.0	36.0	36.0	32.0	36.0
33	34.83753450044349	36.0	36.0	36.0	32.0	36.0
34	34.823469840294536	36.0	36.0	36.0	32.0	36.0
35	34.79641641353953	36.0	36.0	36.0	32.0	36.0
36	34.77390626780768	36.0	36.0	36.0	32.0	36.0
37	34.76392343750774	36.0	36.0	36.0	32.0	36.0
38	34.725830488233925	36.0	36.0	36.0	32.0	36.0
39	34.70738235162951	36.0	36.0	36.0	32.0	36.0
40	34.68977659569197	36.0	36.0	36.0	32.0	36.0
41	34.66975085352982	36.0	36.0	36.0	32.0	36.0
42	34.63324665896962	36.0	36.0	36.0	32.0	36.0
43	34.62747759019667	36.0	36.0	36.0	32.0	36.0
44	34.602265012957794	36.0	36.0	36.0	32.0	36.0
45	34.554818541294	36.0	36.0	36.0	32.0	36.0
46	34.557609546650546	36.0	36.0	36.0	32.0	36.0
47	34.52943624912665	36.0	36.0	36.0	32.0	36.0
48	34.49397140365395	36.0	36.0	36.0	32.0	36.0
49	34.48703724809101	36.0	36.0	36.0	32.0	36.0
50	33.98107430788518	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	30.0
22	130.0
23	348.0
24	957.0
25	2285.0
26	4750.0
27	9088.0
28	15232.0
29	23880.0
30	34589.0
31	49529.0
32	73524.0
33	118648.0
34	264460.0
35	1017015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.29584667584768	17.767737722873424	11.67171870169824	26.26469689958066
2	16.397641489857897	19.94302559016953	36.460401298555695	27.19893162141688
3	18.871476150274116	23.267736702544436	28.365778590156605	29.495008557024843
4	12.668887007290952	15.09885305800614	35.38245129816837	36.84980863653454
5	14.76594205412048	36.20589270052376	33.20664588794355	15.821519357412207
6	35.39971829624977	34.5338570991451	16.20663725748887	13.859787347116253
7	30.99347650501216	30.077883047832355	20.146029166191795	18.782611280963685
8	28.475749347154984	32.735346292781784	19.142481257030163	19.64642310303307
9	27.13809839997225	14.274512038610766	18.583474968906245	40.00391459251074
10	16.12260850606266	26.38286696827198	31.18536586574434	26.309158659921017
11	37.77575578888949	21.061065165577354	21.808182489383526	19.354996556149626
12	24.878288381588533	23.415333310209157	28.205258437433418	23.501119870768893
13	29.420144790371094	19.051305937792666	25.440577476723043	26.0879717951132
14	23.5506097349474	19.442517429847033	24.704671248556803	32.30220158664876
15	25.338376881110356	27.163060121203713	22.14166612985546	25.356896867830475
16	26.05204673726147	25.660897184962018	23.604001803685662	24.68305427409085
17	24.188836969609877	25.882703447319	25.17727157857182	24.7511880044993
18	25.232955418242003	24.51693185140405	26.16545842851409	24.08465430183986
19	25.48052861864436	25.101767017328264	25.240512068341847	24.177192295685522
20	25.663127016139022	24.201596559122734	25.151194941751854	24.98408148298639
21	26.53300893419025	24.162388694260414	24.51519753826638	24.789404833282955
22	25.685177568889394	24.3325372010168	24.95521755719517	25.027067672898635
23	24.289984589388975	24.133958346753616	25.446213994420468	26.12984306943694
24	24.752488739352557	24.99696495200908	25.20012734813611	25.050418960502256
25	25.112234835909202	24.292462179585648	25.150575544202685	25.44472744030246
26	24.521577333022808	25.06193975491678	25.666162064129942	24.750320847930467
27	25.317441243948487	24.702317537869966	25.048932406384257	24.931308811797294
28	24.602222893924452	24.45220480751602	25.605956622350835	25.339615676208695
29	24.556821053570456	24.720589765570416	25.633086235004388	25.08950294585474
30	24.563138908571965	24.906285150810913	25.80428771759436	24.72628822302276
31	25.051286117071093	24.678532671981923	24.844778974178556	25.42540223676843
32	24.750940245479637	24.738056776456947	24.872961562665687	25.638041415397726
33	24.400794810935093	24.541088355821593	25.346800687779037	25.711316145464274
34	25.072221754232967	24.67109990139191	25.66380835344311	24.59286999093202
35	25.61376103147035	24.536814512732334	25.38922941989703	24.46019503590028
36	24.56883736602431	25.0103439390711	25.18228869872008	25.23852999618451
37	25.58570232249305	24.61151385716197	25.041623515304074	24.761160305040907
38	24.758311076314733	24.511852791500875	25.2602708501603	25.469565282024092
39	25.23320317726167	24.45121377143735	24.883739080021208	25.431843971279772
40	25.442621488635293	24.610275062063636	25.37368254141292	24.573420907888153
41	24.404387316720268	24.797518941177053	25.872235628738068	24.925858113364615
42	25.633953391573222	25.013750625591523	25.262686500602054	24.0896094822332
43	24.823843337016687	23.987037248091017	25.430605176181437	25.758514238710863
44	24.285524927034967	24.66533950418465	25.507720171052828	25.54141539772755
45	24.47567997462948	24.832267143685367	25.417412008384165	25.274640873300992
46	24.338731176508478	24.369886873231618	25.755726949739604	25.535655000520297
47	24.599187845933532	24.365736909652195	25.990726379893857	25.044348864520412
48	25.15051360444777	25.168166434599055	24.836726806039376	24.844593154913806
49	24.19323469220897	25.07624783830255	25.37640789062926	25.35410957885922
50	24.137365033274037	25.415677695246497	25.11130573958545	25.335651531894023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	19.0
2	29.0
3	102.5
4	176.0
5	216.5
6	257.0
7	233.5
8	210.0
9	316.5
10	423.0
11	608.5
12	794.0
13	1389.0
14	1984.0
15	2625.0
16	3266.0
17	3686.0
18	4106.0
19	4448.5
20	4791.0
21	5505.5
22	6220.0
23	6937.5
24	7655.0
25	8750.5
26	9846.0
27	12063.5
28	14281.0
29	16650.0
30	19019.0
31	21420.0
32	23821.0
33	27754.5
34	31688.0
35	37236.0
36	42784.0
37	49885.5
38	56987.0
39	62597.5
40	68208.0
41	74663.5
42	81119.0
43	84283.0
44	87447.0
45	97120.0
46	106793.0
47	114701.5
48	122610.0
49	125414.5
50	128219.0
51	123384.5
52	118550.0
53	116711.0
54	114872.0
55	115366.5
56	115861.0
57	112125.5
58	108390.0
59	100534.0
60	92678.0
61	81238.5
62	69799.0
63	60878.5
64	51958.0
65	44353.5
66	36749.0
67	31598.0
68	26447.0
69	23974.5
70	21502.0
71	16598.0
72	11694.0
73	10469.5
74	9245.0
75	6891.5
76	4538.0
77	4024.5
78	3511.0
79	2986.5
80	2462.0
81	1914.5
82	1367.0
83	1195.0
84	1023.0
85	846.0
86	669.0
87	480.0
88	291.0
89	183.0
90	75.0
91	49.5
92	24.0
93	19.5
94	15.0
95	10.0
96	5.0
97	4.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01678567358244677
2	0.0039022045597569975
3	5.574577942509997E-4
4	3.096987745838887E-4
5	0.0
6	6.193975491677774E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1614472.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.15244445976997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68496414408457	43.151355950913015
2	11.88582847124515	12.872933322949113
3	3.4125711869024467	5.543972149912381
4	1.4762724241218983	3.1977504181900427
5	0.8201361230632285	2.2206187926816274
6	0.5108875956248551	1.6599487288356232
7	0.3680285879378457	1.3950753367538231
8	0.2688281556558285	1.164616141469972
9	0.2136880459517286	1.041455703610598
>10	1.1876221047562088	11.594222262970357
>50	0.08636588429893283	3.212782749624332
>100	0.07445109921347312	8.392656441450507
>500	0.00793973581035077	2.8523537089450492
>1k	0.002416441333585017	1.7002582916936015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1825	0.11304005272311937	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1773	0.10981918546744693	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1694	0.1049259448290215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.193975491677775E-5
2	0.0	0.0	0.0	0.0	6.193975491677775E-5
3	0.0	0.0	0.0	0.0	6.193975491677775E-5
4	0.0	0.0	0.0	6.193975491677775E-5	6.193975491677775E-5
5	0.0	0.0	0.0	1.238795098335555E-4	1.8581926475033325E-4
6	0.0	0.0	0.0	1.238795098335555E-4	1.8581926475033325E-4
7	0.0	0.0	0.0	1.238795098335555E-4	1.8581926475033325E-4
8	0.0	0.0	0.0	1.238795098335555E-4	1.8581926475033325E-4
9	0.0	0.0	0.0	1.238795098335555E-4	1.8581926475033325E-4
10	0.0	0.0	0.0	1.8581926475033325E-4	1.8581926475033325E-4
11	0.0	0.0	0.0	1.8581926475033325E-4	1.8581926475033325E-4
12	0.0	0.0	0.0	1.8581926475033325E-4	3.0969877458388874E-4
13	0.0	0.0	0.0	1.8581926475033325E-4	3.0969877458388874E-4
14	0.0	0.0	0.0	1.8581926475033325E-4	3.716385295006665E-4
15	0.0	0.0	0.0	2.47759019667111E-4	3.716385295006665E-4
16	0.0	0.0	0.0	3.0969877458388874E-4	3.716385295006665E-4
17	0.0	0.0	0.0	3.0969877458388874E-4	3.716385295006665E-4
18	0.0	0.0	0.0	3.0969877458388874E-4	3.716385295006665E-4
19	0.0	0.0	0.0	3.0969877458388874E-4	3.716385295006665E-4
20	0.0	0.0	0.0	5.574577942509997E-4	3.716385295006665E-4
21	0.0	0.0	0.0	8.671565688348885E-4	3.716385295006665E-4
22	0.0	0.0	0.0	0.0011768553434187772	3.716385295006665E-4
23	0.0	0.0	0.0	0.0022917709319207765	5.574577942509997E-4
24	0.0	0.0	0.0	0.003406686520422776	5.574577942509997E-4
25	0.0	0.0	0.0	0.004088023824507331	5.574577942509997E-4
26	0.0	0.0	0.0	0.005512638187593219	5.574577942509997E-4
27	0.0	0.0	0.0	0.008114107894097885	5.574577942509997E-4
28	0.0	0.0	0.0	0.015608818239027992	5.574577942509997E-4
29	0.0	0.0	0.0	0.03103181721330565	5.574577942509997E-4
30	0.0	0.0	0.0	0.05407340604234697	5.574577942509997E-4
31	0.0	0.0	0.0	0.11173931786986706	6.193975491677775E-4
32	0.0	0.0	0.0	0.17683800028740046	6.193975491677775E-4
33	0.0	0.0	0.0	0.24311353804835265	6.193975491677775E-4
34	0.0	0.0	0.0	0.31892779806648863	6.193975491677775E-4
35	0.0	0.0	0.0	0.4051479369106432	6.193975491677775E-4
36	0.0	0.0	0.0	0.5269214950770282	6.193975491677775E-4
37	0.0	0.0	0.0	0.7157138680633669	6.193975491677775E-4
38	0.0	0.0	0.0	0.9621721528772255	6.193975491677775E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	60	2.8675335E-4	22.006601	1
TAGGACG	300	0.0	19.799807	4
CCTAATA	115	2.020679E-7	19.132023	2
CGTCGTA	315	0.0	18.856958	10
GTATAGG	155	7.185008E-10	18.457148	1
CTAGATA	370	0.0	18.432823	3
TATACCG	85	1.4313172E-4	18.117468	5
GTCGATA	125	4.855465E-7	17.599827	36
TTAGCCG	115	4.2750817E-6	17.21722	32
ACCGTCG	345	0.0	17.21722	8
TCTAGAT	400	0.0	17.051416	2
TACCGTC	375	0.0	17.013165	7
TTAGGAC	250	0.0	16.720352	3
TGGCGAG	430	0.0	16.371931	18
CCGTCGT	350	0.0	16.342697	9
TTGCGCG	395	0.0	16.15174	18
CGCCCCT	315	0.0	16.063335	5
CGTCTAT	110	5.6087134E-5	16.004799	1
TATGGCG	440	0.0	15.999842	16
ATACCGT	400	0.0	15.949842	6
>>END_MODULE