FastQCFastQC Report
Thu 2 Feb 2017
SRR4062417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990015
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG142411.4384630535900973No Hit
TCCATGTACTCTGCGTTGATACCAC124011.2526072837280242No Hit
GAGTACATGGAAGCAGTGGTATCAA62520.6315055832487386No Hit
CATGTACTCTGCGTTGATACCACTG56690.5726175866022232No Hit
GTCCTACAGTGGACATTTCTAAATT47540.4801947445240729No Hit
GTCCTAAAGTGTGTATTTCTCATTT45910.46373034751998704No Hit
GTCCTACAGTGTGCATTTCTCATTT44600.45049822477437207No Hit
CTGTAGGACGTGGAATATGGCAAGA43760.44201350484588614No Hit
GTATCAACGCAGAGTACTTTTTTTT43530.4396903077226103No Hit
GCTTCCATGTACTCTGCGTTGATAC41240.41655934506042835No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41040.41453917364888415No Hit
CCCATGTACTCTGCGTTGATACCAC38180.38565072246380105No Hit
CTTTAGGACGTGAAATATGGCGAGG36460.36827724832452036No Hit
CTGTAGGACCTGGAATATGGCGAGA34720.3507017570440852No Hit
GCGTTGATACCACTGCTTCCATGTA34150.34494426852118404No Hit
GTACATGGGAAGCAGTGGTATCAAC32970.3330252571930728No Hit
CATGGAAGCAGTGGTATCAACGCAG32690.33019701721691086No Hit
TATCAACGCAGAGTACTTTTTTTTT30980.3129245516482073No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30730.310399337383777No Hit
GTATCAACGCAGAGTACATGGAAGC28520.2880764432862128No Hit
GATATACACTGTTCTACAAATCCCG26160.2642384206299904No Hit
TTGTAGAACAGTGTATATCAATGAG25320.2557537007015045No Hit
ACTCTGCGTTGATACCACTGCTTCC23940.24181451796184908No Hit
CCATAGGGTCTTCTCGTCTTATTAT22950.23181466947470494No Hit
ACGCAGAGTACATGGAAGCAGTGGT22410.2263602066635354No Hit
CAGTGGTATCAACGCAGAGTACATG20640.20848168967136863No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20580.20787563824790534No Hit
GTATTAGAGGCACTGCCTGCCCAGT19820.20019898688403712No Hit
GTGTATATCAATGAGTTACAATGAA19790.1998959611723055No Hit
CTGAAGGACCTGGAATATGGCGAGA19310.19504754978459923No Hit
TATCAACGCAGAGTACATGGAAGCA18520.18706787270899936No Hit
GTGTATATCAATGAGTTACAATGAG17990.18171441846840705No Hit
GTATCAACGCAGAGTACATGGGAAG17670.17848214420993622No Hit
GTACATGGGGTGGTATCAACGCAAA17470.17646197279839196No Hit
GTCCTTCAGTGTGCATTTCTCATTT17380.175552895663197No Hit
GTTCTACAGTGTGGTTTTTATCATT17000.17171456998126292No Hit
ATTTAGAAATGTCCACTGTAGGACG16030.1619167386352732No Hit
GGTATCAACGCAGAGTACTTTTTTT15840.15999757579430615No Hit
CTCTAATACTTGTAATGCTAGAGGT15580.1573713529592986No Hit
CCATTGGGATGTCCTGATCCAACAT15510.1566642929652581No Hit
GATATACACTGTTCTACAATGCCGG15110.15262395014216956No Hit
CTTCTACACCATTGGGATGTCCTGA14450.14595738448407347No Hit
ATCTATAACTTTATAGATGCAACAC13790.13929081882597738No Hit
TTTCTAAATTTTCCACCTTTTTCAG13770.13908880168482296No Hit
ATACCACTGCTTCCATGTACTCTGC13430.1356545102851977No Hit
GGTATCAACGCAGAGTACATGGAAG12930.130604081756337No Hit
GAATATGGCAAGAAAACTGAAAATC12760.12888693605652438No Hit
GTCAGGATACCGCGGCCGTTAAACT12730.12858391034479275No Hit
CTGTTAGTATGAGTAACAAGAATTC12480.1260586960803624No Hit
GAGTACATGGGAAGCAGTGGTATCA12330.12454356752170423No Hit
GATATATTTTGATCAACGGACCAAG12170.1229274303924688No Hit
ATCCTGACCGTGCAAAGGTAGCATA12120.12242238753958275No Hit
GTTCTACAAATCCCGTTTCCAACGA12030.12151331040438781No Hit
CTGTAGGACATGGAATATGGCAAGA11870.1198971732751524No Hit
GCGTTGATACCACTGCTTCCCATGT11610.11727095044014484No Hit
AAGCAGTGGTATCAACGCAGAGTAC11470.11585683045206385No Hit
CTGTAGAACATATTAGATGAGTGAG11240.11353363332878795No Hit
GTTATATAATTTAAGCTCCATAGGG11240.11353363332878795No Hit
GTACATGGGTGGTATCAACGCAAAA11220.11333161618763352No Hit
GTATCCTGACCGTGCAAAGGTAGCA10930.11040236764089434No Hit
GGTCAGGATACCGCGGCCGTTAAAC10810.10919026479396776No Hit
GTTATAGATTAACCCAATTTTAAGT10530.1063620248178058No Hit
CCTCTAGCATTACAAGTATTAGAGG10480.10585698196491972No Hit
GTTCATGCTAGTCCCTAATTAAGGA10460.1056549648237653No Hit
GGAATATGGCGAGAAAACTGAAAAT10440.10545294768261088No Hit
TATCAACGCAGAGTACATGGGAAGC10400.10504891340030202No Hit
GGTATCAACGCAGAGTACATGGGAA10240.10343277627106659No Hit
ACCTATAACTTCTCTGTTAACCCAA10080.10181663914183119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGG250.00604466818.9923132
AGGCGAG704.4236185E-714.93083818
ATAGGAC1550.014.7059573
TAGGACC12300.014.672574
CCCAATT1250.014.43999913
GGGGTCC400.005271994514.2521636
TTGCGGT602.5627241E-514.25216317
GACGGTG400.005271994514.2521637
TAAGGCG602.5627241E-514.25216316
CTAGGAC2000.014.2463973
TCCAACG3950.013.95148518
TGTCGAG551.956496E-413.82027918
CCAACGA2750.013.81957919
AGAACGT551.9598573E-413.8174835
GAATTAG707.244784E-613.5734887
TAGGCAA1700.013.41386
TAACTTT2200.013.3883956
TAGAAAT4900.013.3755214
AACGGAC1351.8189894E-1213.37239915
CTCTAGG1850.013.3425781
Produced by FastQC (version 0.11.5)