##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062417_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	990015
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07212516982066	32.0	32.0	32.0	32.0	32.0
2	30.910992257693064	32.0	32.0	32.0	32.0	32.0
3	30.89346929086933	32.0	32.0	32.0	32.0	32.0
4	30.900800492921825	32.0	32.0	32.0	32.0	32.0
5	30.84649222486528	32.0	32.0	32.0	32.0	32.0
6	34.425871325181944	36.0	36.0	36.0	32.0	36.0
7	34.256275915011386	36.0	36.0	36.0	32.0	36.0
8	34.251816386620405	36.0	36.0	36.0	32.0	36.0
9	34.33145861426342	36.0	36.0	36.0	32.0	36.0
10	34.13599187891093	36.0	36.0	36.0	32.0	36.0
11	34.411907900385344	36.0	36.0	36.0	32.0	36.0
12	34.23216011878608	36.0	36.0	36.0	32.0	36.0
13	34.311316495204615	36.0	36.0	36.0	32.0	36.0
14	34.24401448462902	36.0	36.0	36.0	32.0	36.0
15	34.231934869673694	36.0	36.0	36.0	32.0	36.0
16	34.23193891001652	36.0	36.0	36.0	32.0	36.0
17	34.18990823371363	36.0	36.0	36.0	32.0	36.0
18	34.23324394074837	36.0	36.0	36.0	32.0	36.0
19	34.15181184123473	36.0	36.0	36.0	32.0	36.0
20	34.10509032691424	36.0	36.0	36.0	32.0	36.0
21	34.02500972207492	36.0	36.0	36.0	32.0	36.0
22	33.983442675110986	36.0	36.0	36.0	32.0	36.0
23	33.93883021974415	36.0	36.0	36.0	32.0	36.0
24	33.929132386883026	36.0	36.0	36.0	32.0	36.0
25	33.47811194779877	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	4.0
4	43.0
5	101.0
6	156.0
7	48.0
8	128.0
9	173.0
10	105.0
11	31.0
12	79.0
13	44.0
14	222.0
15	293.0
16	403.0
17	602.0
18	749.0
19	1093.0
20	1694.0
21	2325.0
22	3305.0
23	4673.0
24	6210.0
25	7830.0
26	10700.0
27	13874.0
28	18327.0
29	24791.0
30	32687.0
31	45148.0
32	65174.0
33	95419.0
34	215531.0
35	438050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90589035104112	19.980988765997164	12.733991217658428	24.379129665303285
2	11.378287686038737	22.006460723528306	45.25961078080207	21.355640809630884
3	19.53495278616192	27.61494357370571	30.20099720797928	22.649106432153086
4	10.144598104555552	18.435374355834792	40.59718320104016	30.822844338569492
5	9.498158449156524	41.26003506272388	37.7386058778059	11.503200610313694
6	28.214067723302165	39.55026294621022	20.002910396177683	12.232758934309933
7	24.820439113040983	33.5045554794119	23.294803296683302	18.380202110863813
8	23.256389959653	40.48175792003186	21.74876082853385	14.513091291781283
9	26.03928554458168	16.343061538725955	21.200222016202332	36.41743090049003
10	14.358363476222594	29.126879232602658	36.725112018291014	19.789645272883735
11	32.205821975297155	22.30961679647761	27.45951455898325	18.025046669241988
12	22.9755028435554	26.117826255376862	34.134498198188176	16.772172702879562
13	29.912567046088878	23.144552581122735	27.459772346673788	19.483108026114603
14	19.785907516496852	25.436047834551108	27.6590903344879	27.11895431446414
15	21.911409890040996	34.62909818245065	26.211783835601477	17.24770809190688
16	20.04331853991039	28.064023265008547	33.50614235459117	18.386515840489892
17	18.11237827675585	30.254616356246476	32.59336118468358	19.03964418231409
18	19.09749753232253	26.76024288418907	37.61589993365588	16.52635964983252
19	22.310257325685946	29.41187179328989	27.90400375424654	20.37386712677763
20	20.819108486368417	28.927069007361865	33.3916147560877	16.862207750182023
21	20.152004733081508	28.38463366657059	26.908276318916652	24.555085281431253
22	19.81874719289798	33.18348634989743	27.95934304708648	19.03842341011811
23	18.717280134641992	31.823128646216837	29.419052974827448	20.040538244313723
24	21.04254953323961	31.041841554317156	30.111152690825605	17.80445622161763
25	21.131368664205013	29.934352582559896	30.286204940092055	18.648073813143036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	222.0
1	222.0
2	719.5
3	1217.0
4	1217.0
5	1217.0
6	3974.5
7	6732.0
8	6732.0
9	6732.0
10	6486.5
11	6241.0
12	6241.0
13	6241.0
14	5831.0
15	5421.0
16	5421.0
17	5421.0
18	9358.5
19	13296.0
20	13296.0
21	13296.0
22	22810.0
23	32324.0
24	32324.0
25	32324.0
26	52267.5
27	72211.0
28	72211.0
29	72211.0
30	94553.5
31	116896.0
32	116896.0
33	116896.0
34	119256.0
35	121616.0
36	121616.0
37	121616.0
38	118037.5
39	114459.0
40	114459.0
41	114459.0
42	115351.0
43	116243.0
44	116243.0
45	116243.0
46	148572.5
47	180902.0
48	180902.0
49	180902.0
50	141759.0
51	102616.0
52	102616.0
53	102616.0
54	74342.5
55	46069.0
56	46069.0
57	46069.0
58	35236.0
59	24403.0
60	24403.0
61	24403.0
62	19036.5
63	13670.0
64	13670.0
65	13670.0
66	10656.0
67	7642.0
68	7642.0
69	7642.0
70	5845.5
71	4049.0
72	4049.0
73	4049.0
74	3032.0
75	2015.0
76	2015.0
77	2015.0
78	1409.5
79	804.0
80	804.0
81	804.0
82	553.0
83	302.0
84	302.0
85	302.0
86	211.0
87	120.0
88	120.0
89	120.0
90	82.0
91	44.0
92	44.0
93	44.0
94	36.0
95	28.0
96	28.0
97	28.0
98	250.5
99	473.0
100	473.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007474634222713798
2	0.0033332828290480445
3	0.005151437099437888
4	0.016969439856971864
5	0.03636308540779685
6	0.046362933894940984
7	0.06636263086922925
8	0.0854532507083226
9	0.09070569637833771
10	0.11252354762301582
11	0.11525077902860058
12	0.13009903890345095
13	0.115452796169755
14	0.12020019898688404
15	0.11121043620551203
16	0.1072711019530007
17	0.1206042332691929
18	0.12434155038054978
19	0.1280788674919067
20	0.1146447276051373
21	0.12424054180997257
22	0.14656343590753676
23	0.13323030459134458
24	0.12979601319171932
25	0.1260586960803624
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	990015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.22026840522457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35100136810918	20.135444095895863
2	14.577018692814914	8.227347601184253
3	5.388525441599505	4.561969028109578
4	2.40042466992205	2.7096251388689137
5	1.3422780104411334	1.8939722864539803
6	0.850704728773601	1.4404269467750872
7	0.5818761626314428	1.1494491041642994
8	0.4209930761415134	0.9504430084363721
9	0.31232830312819726	0.793258969034346
>10	2.1688840834685172	12.005548195812718
>50	0.27824213335703313	5.561307854560076
>100	0.2671267839843072	15.789536345404919
>500	0.036214503600557	7.421316834873701
>1k	0.02294780426173909	13.458302027765113
>5k	7.171188831793466E-4	1.2062435131986609
>10k+	7.171188831793466E-4	2.6958090494621865
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14241	1.4384630535900973	No Hit
TCCATGTACTCTGCGTTGATACCAC	12401	1.2526072837280242	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6252	0.6315055832487386	No Hit
CATGTACTCTGCGTTGATACCACTG	5669	0.5726175866022232	No Hit
GTCCTACAGTGGACATTTCTAAATT	4754	0.4801947445240729	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4591	0.46373034751998704	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4460	0.45049822477437207	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4376	0.44201350484588614	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4353	0.4396903077226103	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4124	0.41655934506042835	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4104	0.41453917364888415	No Hit
CCCATGTACTCTGCGTTGATACCAC	3818	0.38565072246380105	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3646	0.36827724832452036	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3472	0.3507017570440852	No Hit
GCGTTGATACCACTGCTTCCATGTA	3415	0.34494426852118404	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3297	0.3330252571930728	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3269	0.33019701721691086	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3098	0.3129245516482073	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3073	0.310399337383777	No Hit
GTATCAACGCAGAGTACATGGAAGC	2852	0.2880764432862128	No Hit
GATATACACTGTTCTACAAATCCCG	2616	0.2642384206299904	No Hit
TTGTAGAACAGTGTATATCAATGAG	2532	0.2557537007015045	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2394	0.24181451796184908	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2295	0.23181466947470494	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2241	0.2263602066635354	No Hit
CAGTGGTATCAACGCAGAGTACATG	2064	0.20848168967136863	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2058	0.20787563824790534	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1982	0.20019898688403712	No Hit
GTGTATATCAATGAGTTACAATGAA	1979	0.1998959611723055	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1931	0.19504754978459923	No Hit
TATCAACGCAGAGTACATGGAAGCA	1852	0.18706787270899936	No Hit
GTGTATATCAATGAGTTACAATGAG	1799	0.18171441846840705	No Hit
GTATCAACGCAGAGTACATGGGAAG	1767	0.17848214420993622	No Hit
GTACATGGGGTGGTATCAACGCAAA	1747	0.17646197279839196	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1738	0.175552895663197	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1700	0.17171456998126292	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1603	0.1619167386352732	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1584	0.15999757579430615	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1558	0.1573713529592986	No Hit
CCATTGGGATGTCCTGATCCAACAT	1551	0.1566642929652581	No Hit
GATATACACTGTTCTACAATGCCGG	1511	0.15262395014216956	No Hit
CTTCTACACCATTGGGATGTCCTGA	1445	0.14595738448407347	No Hit
ATCTATAACTTTATAGATGCAACAC	1379	0.13929081882597738	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1377	0.13908880168482296	No Hit
ATACCACTGCTTCCATGTACTCTGC	1343	0.1356545102851977	No Hit
GGTATCAACGCAGAGTACATGGAAG	1293	0.130604081756337	No Hit
GAATATGGCAAGAAAACTGAAAATC	1276	0.12888693605652438	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1273	0.12858391034479275	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1248	0.1260586960803624	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1233	0.12454356752170423	No Hit
GATATATTTTGATCAACGGACCAAG	1217	0.1229274303924688	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1212	0.12242238753958275	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1203	0.12151331040438781	No Hit
CTGTAGGACATGGAATATGGCAAGA	1187	0.1198971732751524	No Hit
GCGTTGATACCACTGCTTCCCATGT	1161	0.11727095044014484	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1147	0.11585683045206385	No Hit
CTGTAGAACATATTAGATGAGTGAG	1124	0.11353363332878795	No Hit
GTTATATAATTTAAGCTCCATAGGG	1124	0.11353363332878795	No Hit
GTACATGGGTGGTATCAACGCAAAA	1122	0.11333161618763352	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1093	0.11040236764089434	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1081	0.10919026479396776	No Hit
GTTATAGATTAACCCAATTTTAAGT	1053	0.1063620248178058	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1048	0.10585698196491972	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1046	0.1056549648237653	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1044	0.10545294768261088	No Hit
TATCAACGCAGAGTACATGGGAAGC	1040	0.10504891340030202	No Hit
GGTATCAACGCAGAGTACATGGGAA	1024	0.10343277627106659	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1008	0.10181663914183119	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.0100857057721348E-4	0.0
4	0.0	0.0	0.0	1.0100857057721348E-4	0.0
5	0.0	0.0	0.0	1.0100857057721348E-4	0.0
6	0.0	0.0	0.0	1.0100857057721348E-4	0.0
7	0.0	0.0	0.0	4.040342823088539E-4	0.0
8	0.0	0.0	0.0	5.050428528860674E-4	0.0
9	0.0	0.0	0.0	5.050428528860674E-4	0.0
10	0.0	0.0	0.0	5.050428528860674E-4	0.0
11	0.0	0.0	0.0	5.050428528860674E-4	0.0
12	0.0	0.0	0.0	5.050428528860674E-4	0.0
13	0.0	0.0	0.0	6.060514234632809E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTAGG	25	0.006044668	18.992313	2
AGGCGAG	70	4.4236185E-7	14.930838	18
ATAGGAC	155	0.0	14.705957	3
TAGGACC	1230	0.0	14.67257	4
CCCAATT	125	0.0	14.439999	13
GGGGTCC	40	0.0052719945	14.252163	6
TTGCGGT	60	2.5627241E-5	14.252163	17
GACGGTG	40	0.0052719945	14.252163	7
TAAGGCG	60	2.5627241E-5	14.252163	16
CTAGGAC	200	0.0	14.246397	3
TCCAACG	395	0.0	13.951485	18
TGTCGAG	55	1.956496E-4	13.820279	18
CCAACGA	275	0.0	13.819579	19
AGAACGT	55	1.9598573E-4	13.817483	5
GAATTAG	70	7.244784E-6	13.573488	7
TAGGCAA	170	0.0	13.4138	6
TAACTTT	220	0.0	13.388395	6
TAGAAAT	490	0.0	13.375521	4
AACGGAC	135	1.8189894E-12	13.372399	15
CTCTAGG	185	0.0	13.342578	1
>>END_MODULE