FastQCFastQC Report
Thu 2 Feb 2017
SRR4062417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990015
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA80630.8144321045640723No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC65270.6592829401574724No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC30280.3058539517078024No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA29900.3020156260258683No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23850.24090544082665416No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG22010.22231986384044686No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20340.20545143255405218No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG19240.19434048979055873No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19180.19373443836709545No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT18990.1918152755261284No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA18740.18929006126169803No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC17640.1781791184982046No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA17150.17322969853992112No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16360.16525002146432125No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15690.15848244723564794No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15660.15817942152391629No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT15510.1566642929652581No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG14780.14929066731312152No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA14300.14444225592541526No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC14210.14353317879022034No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA13750.13888678454366854No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA13050.13181618460326358No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12840.1296950046211421No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA12090.12211936182785109No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC12080.12201835325727388No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT11870.1198971732751524No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC11550.11666489901668156No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC11450.11565481331090943No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG11400.11514977045802335No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA11320.11434170189340565No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA11250.11363464189936515No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC11180.11292758190532466No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC10740.10848320479992728No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC10730.10838219622935005No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA10460.1056549648237653No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA10420.10525093054145644No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA10410.10514992197087922No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA9930.10030151058317298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCA454.069224E-529.33289530
TCGTATA703.5899575E-828.28529244
CTCGTAT554.960435E-627.99958243
TAGGACC14650.027.6323854
ATAGGAC1950.027.0778873
CCGAGTG508.340777E-526.3996079
ACTCGTA508.340777E-526.39960742
TAGGGCC1107.2759576E-1226.0009254
CTGTAGG33800.024.2212181
GCACTCG551.5934955E-423.99964340
TGTAGGA35050.023.790922
CACTCGT651.8131706E-523.69195241
ACTGTTC10050.023.6414388
AGGACCT23250.023.2782465
TGGTATA4400.022.99965744
TAGGACA8850.022.870874
GGACCTG23300.022.85066
AGGGCCT1355.456968E-1222.8156265
GATATAC11700.022.3836481
ATACACT12150.022.2723984
Produced by FastQC (version 0.11.5)