##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	990015
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18467194941491	32.0	32.0	32.0	32.0	32.0
2	31.493909688237046	32.0	32.0	32.0	32.0	32.0
3	31.570588324419326	32.0	32.0	32.0	32.0	32.0
4	31.662500063130356	32.0	32.0	32.0	32.0	32.0
5	31.63380150805796	32.0	32.0	32.0	32.0	32.0
6	35.23092983439645	36.0	36.0	36.0	36.0	36.0
7	35.25154972399408	36.0	36.0	36.0	36.0	36.0
8	35.19271728206138	36.0	36.0	36.0	36.0	36.0
9	35.288348156341065	36.0	36.0	36.0	36.0	36.0
10	35.22338651434574	36.0	36.0	36.0	36.0	36.0
11	35.30334893915749	36.0	36.0	36.0	36.0	36.0
12	35.23209850355803	36.0	36.0	36.0	36.0	36.0
13	35.260925339515055	36.0	36.0	36.0	36.0	36.0
14	35.236544900834836	36.0	36.0	36.0	36.0	36.0
15	35.206456467831295	36.0	36.0	36.0	36.0	36.0
16	35.21997141457452	36.0	36.0	36.0	36.0	36.0
17	35.22440266056575	36.0	36.0	36.0	36.0	36.0
18	35.222719857779936	36.0	36.0	36.0	36.0	36.0
19	35.170859027388474	36.0	36.0	36.0	36.0	36.0
20	35.122797129336426	36.0	36.0	36.0	36.0	36.0
21	35.059190012272545	36.0	36.0	36.0	36.0	36.0
22	35.032038908501384	36.0	36.0	36.0	36.0	36.0
23	34.99219405766579	36.0	36.0	36.0	36.0	36.0
24	34.98175583198234	36.0	36.0	36.0	36.0	36.0
25	34.95954000696959	36.0	36.0	36.0	36.0	36.0
26	34.9133437372161	36.0	36.0	36.0	36.0	36.0
27	34.852606273642316	36.0	36.0	36.0	36.0	36.0
28	34.793652621424926	36.0	36.0	36.0	32.0	36.0
29	34.7496714696242	36.0	36.0	36.0	32.0	36.0
30	34.70054595132397	36.0	36.0	36.0	32.0	36.0
31	34.674445336686816	36.0	36.0	36.0	32.0	36.0
32	34.63009045317495	36.0	36.0	36.0	32.0	36.0
33	34.601466644444784	36.0	36.0	36.0	32.0	36.0
34	34.54640081210891	36.0	36.0	36.0	32.0	36.0
35	34.44833462119261	36.0	36.0	36.0	32.0	36.0
36	34.37768518658808	36.0	36.0	36.0	32.0	36.0
37	34.262984904269125	36.0	36.0	36.0	32.0	36.0
38	34.17611955374414	36.0	36.0	36.0	32.0	36.0
39	33.92318702241885	36.0	36.0	36.0	32.0	36.0
40	33.86297177315495	36.0	36.0	36.0	32.0	36.0
41	33.87823618834058	36.0	36.0	36.0	32.0	36.0
42	33.69758741029176	36.0	36.0	36.0	27.0	36.0
43	33.730336409044305	36.0	36.0	36.0	27.0	36.0
44	33.68992186987066	36.0	36.0	36.0	27.0	36.0
45	33.4611596793988	36.0	36.0	36.0	21.0	36.0
46	33.470568627748065	36.0	36.0	36.0	21.0	36.0
47	33.3857557713772	36.0	36.0	36.0	21.0	36.0
48	33.35829558138008	36.0	36.0	36.0	21.0	36.0
49	33.37047519482028	36.0	36.0	36.0	21.0	36.0
50	32.80966652020424	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	18.0
22	69.0
23	269.0
24	696.0
25	1900.0
26	4224.0
27	8143.0
28	14681.0
29	21963.0
30	30799.0
31	42943.0
32	62475.0
33	83576.0
34	157207.0
35	561047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52802977342617	20.71037180414517	13.13861534171957	24.622983080709098
2	11.07651933059854	22.352945930083973	44.91246607182722	21.65806866749027
3	19.1321493036932	27.955200407269025	30.581035121902932	22.331615167134842
4	10.06673649739953	18.879348048965014	40.66673871959257	30.387176734042885
5	9.431877294788462	41.6029050064898	37.39640308480175	11.56881461391999
6	28.418601832304724	39.6788921324027	19.70808375672973	12.194422278562843
7	24.853082885696566	33.65901896336819	23.251186346859743	18.236711804075497
8	23.408736231269224	40.69665611127104	21.369171174174127	14.525436483285606
9	25.953647166962117	16.175613500805543	21.187052721423413	36.68368661080893
10	14.516244703363078	29.46753332020222	36.38217602763594	19.634045948798757
11	32.257794073827164	22.350267420190605	27.427564228824814	17.964374277157415
12	23.039650914380086	26.231420736049454	33.96837421655227	16.76055413301819
13	30.0908572092342	23.070054494123827	27.41837244890229	19.420715847739682
14	19.965253051721437	25.532946470508023	27.65766175259971	26.84413872517083
15	22.007949374504427	34.777755892587486	26.028292500618676	17.18600223228941
16	20.08585728499063	28.15452291126902	33.209496825805665	18.550122977934677
17	18.193764740938267	30.516002282793696	32.19324959722832	19.096983379039713
18	19.21809265516179	26.72929198042454	37.42721069882779	16.625404665585876
19	22.512184158825875	29.298849007338273	28.038767089387534	20.150199744448315
20	21.127457664782856	28.993702115624508	33.043135710064995	16.835704509527634
21	20.375852891117813	28.220481507855943	26.937571652954755	24.466093948071492
22	20.049494199582835	32.810108937743365	27.960586455760772	19.179810406913024
23	18.7871900930794	31.763357120851705	29.27794023322879	20.17151255284011
24	21.22341580683121	31.075993798073764	29.71662045524563	17.983969939849395
25	21.464422256228442	29.706721615329062	29.975303404493868	18.853552723948628
26	15.94773816558335	30.875794811189728	33.160608677646294	20.01585834558062
27	19.22677939223143	32.241531693964234	30.910137725185983	17.621551188618355
28	17.900031817699734	30.440245854860787	29.422685514865936	22.237036812573546
29	18.92052140624132	29.575107447866948	29.761973303434797	21.74239784245693
30	20.239794346550305	28.68037352969399	31.229627833921707	19.850204289833993
31	20.51625480422014	27.66665151538108	30.45559915758852	21.361494522810258
32	17.561754114836646	31.95961677348323	31.119528491992543	19.35910061968758
33	17.81094225845063	31.43073589794094	32.01810073584744	18.740221107760995
34	17.503674186754747	31.872648394216252	33.630601556542075	16.993075862486933
35	19.312737685792637	32.23890547112923	30.742867532310115	17.70548931076802
36	17.71235789356727	30.964884370438835	32.31476290763271	19.007994828361184
37	19.412534153522927	30.48165936879744	28.919359807679683	21.18644666999995
38	19.489401675732186	33.02303500452013	27.824729928334417	19.662833391413262
39	19.845759912728596	29.577329636419652	29.86368893400605	20.713221516845703
40	22.828239976161978	29.741165537895892	29.627126861714213	17.803467624227913
41	20.602920157775387	27.617864375792283	32.59536471669621	19.183850749736113
42	20.82069463593986	32.85344161452099	29.72136785806276	16.604495891476393
43	17.785286081524017	31.729418241137758	30.404589829447026	20.080705847891196
44	17.819527986949694	31.25791023368333	31.853153740094847	19.069408039272133
45	18.87597662661677	31.641843810447316	30.40378176088241	19.078397802053505
46	19.402048859915112	28.282731355314166	31.057136565745537	21.25808321902519
47	23.036317631551036	28.405529209153396	29.940152421933004	18.618000737362564
48	18.1621490583476	32.65142447336656	27.767559077387716	21.418867390898118
49	19.565259112235672	28.751382554809773	32.1432503547926	19.54010797816195
50	17.151356292581426	34.42776119553744	26.86878481639167	21.55209769548946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	27.0
2	25.0
3	128.5
4	232.0
5	276.0
6	320.0
7	304.5
8	289.0
9	403.0
10	517.0
11	730.5
12	944.0
13	1708.5
14	2473.0
15	3436.5
16	4400.0
17	5898.5
18	7397.0
19	7984.0
20	8571.0
21	10331.0
22	12091.0
23	12301.5
24	12512.0
25	15149.0
26	17786.0
27	26286.5
28	34787.0
29	40482.5
30	46178.0
31	49644.5
32	53111.0
33	64434.5
34	75758.0
35	86750.0
36	97742.0
37	100747.0
38	103752.0
39	93758.0
40	83764.0
41	77769.5
42	71775.0
43	62748.5
44	53722.0
45	59187.0
46	64652.0
47	69116.5
48	73581.0
49	62146.0
50	50711.0
51	40974.5
52	31238.0
53	25658.0
54	20078.0
55	17977.0
56	15876.0
57	15143.0
58	14410.0
59	11845.0
60	9280.0
61	7993.0
62	6706.0
63	5670.5
64	4635.0
65	4120.5
66	3606.0
67	3008.5
68	2411.0
69	2097.5
70	1784.0
71	1472.0
72	1160.0
73	941.0
74	722.0
75	586.0
76	450.0
77	361.5
78	273.0
79	202.0
80	131.0
81	104.5
82	78.0
83	54.0
84	30.0
85	23.0
86	16.0
87	11.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014040191310232673
2	0.005656479952323955
3	6.060514234632809E-4
4	2.0201714115442693E-4
5	0.0
6	5.050428528860674E-4
7	1.0100857057721347E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0100857057721347E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	990015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.208722503722264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49917112477252	29.210173273885236
2	13.346532573747321	10.466009841418977
3	4.778791865612642	5.621109724855539
4	2.217233257955873	3.477395341488634
5	1.1823338402250794	2.3178899724072712
6	0.7738786869485118	1.820567681286549
7	0.5093784513441532	1.3980454843690084
8	0.37028150254653913	1.1614611745286865
9	0.288323896052156	1.0174330468350945
>10	1.655627076729739	12.380035364320403
>50	0.17914276220082542	4.916765581062743
>100	0.16931874192295757	13.599852958726256
>500	0.02042164978785423	5.657044216820782
>1k	0.009047566361707572	5.47744264101173
>5k	5.170037920975755E-4	1.4787736969831082
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	8063	0.8144321045640723	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	6527	0.6592829401574724	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3028	0.3058539517078024	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2990	0.3020156260258683	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2385	0.24090544082665416	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2201	0.22231986384044686	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	2034	0.20545143255405218	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1924	0.19434048979055873	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1918	0.19373443836709545	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1899	0.1918152755261284	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1874	0.18929006126169803	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1764	0.1781791184982046	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1715	0.17322969853992112	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1636	0.16525002146432125	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1569	0.15848244723564794	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1566	0.15817942152391629	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	1551	0.1566642929652581	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1478	0.14929066731312152	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	1430	0.14444225592541526	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1421	0.14353317879022034	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1375	0.13888678454366854	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	1305	0.13181618460326358	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1284	0.1296950046211421	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	1209	0.12211936182785109	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	1208	0.12201835325727388	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	1187	0.1198971732751524	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	1155	0.11666489901668156	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	1145	0.11565481331090943	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1140	0.11514977045802335	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1132	0.11434170189340565	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1125	0.11363464189936515	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1118	0.11292758190532466	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	1074	0.10848320479992728	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	1073	0.10838219622935005	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	1046	0.1056549648237653	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	1042	0.10525093054145644	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1041	0.10514992197087922	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	993	0.10030151058317298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.0100857057721348E-4	0.0
9	0.0	0.0	0.0	1.0100857057721348E-4	0.0
10	0.0	0.0	0.0	1.0100857057721348E-4	0.0
11	0.0	0.0	0.0	1.0100857057721348E-4	0.0
12	0.0	0.0	0.0	1.0100857057721348E-4	0.0
13	0.0	0.0	0.0	2.0201714115442696E-4	0.0
14	0.0	0.0	0.0	2.0201714115442696E-4	0.0
15	0.0	0.0	0.0	2.0201714115442696E-4	0.0
16	0.0	0.0	0.0	3.0302571173164043E-4	0.0
17	0.0	0.0	0.0	5.050428528860674E-4	0.0
18	0.0	0.0	0.0	6.060514234632809E-4	0.0
19	0.0	0.0	0.0	8.080685646177078E-4	0.0
20	0.0	0.0	0.0	0.0014141199880809887	0.0
21	1.0100857057721348E-4	0.0	0.0	0.001717145699812629	0.0
22	1.0100857057721348E-4	0.0	0.0	0.002929248546739191	0.0
23	1.0100857057721348E-4	0.0	0.0	0.004848411387706247	0.0
24	1.0100857057721348E-4	0.0	0.0	0.00959581420483528	0.0
25	1.0100857057721348E-4	0.0	0.0	0.01191901132811119	0.0
26	1.0100857057721348E-4	0.0	0.0	0.01595935415119973	0.0
27	1.0100857057721348E-4	0.0	0.0	0.02121179982121483	0.0
28	1.0100857057721348E-4	0.0	0.0	0.029595511179123547	0.0
29	1.0100857057721348E-4	0.0	0.0	0.043130659636470156	0.0
30	1.0100857057721348E-4	0.0	0.0	0.06494851088114827	0.0
31	1.0100857057721348E-4	0.0	0.0	0.1056549648237653	0.0
32	1.0100857057721348E-4	0.0	0.0	0.1619167386352732	0.0
33	1.0100857057721348E-4	0.0	0.0	0.21383514391196093	0.0
34	1.0100857057721348E-4	0.0	0.0	0.28383408332196985	0.0
35	1.0100857057721348E-4	0.0	0.0	0.35999454553718885	0.0
36	1.0100857057721348E-4	0.0	0.0	0.4623162275319061	0.0
37	1.0100857057721348E-4	0.0	0.0	0.6254450690141058	0.0
38	1.0100857057721348E-4	0.0	0.0	0.8052403246415458	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCGCA	45	4.069224E-5	29.332895	30
TCGTATA	70	3.5899575E-8	28.285292	44
CTCGTAT	55	4.960435E-6	27.999582	43
TAGGACC	1465	0.0	27.632385	4
ATAGGAC	195	0.0	27.077887	3
CCGAGTG	50	8.340777E-5	26.399607	9
ACTCGTA	50	8.340777E-5	26.399607	42
TAGGGCC	110	7.2759576E-12	26.000925	4
CTGTAGG	3380	0.0	24.221218	1
GCACTCG	55	1.5934955E-4	23.999643	40
TGTAGGA	3505	0.0	23.79092	2
CACTCGT	65	1.8131706E-5	23.691952	41
ACTGTTC	1005	0.0	23.641438	8
AGGACCT	2325	0.0	23.278246	5
TGGTATA	440	0.0	22.999657	44
TAGGACA	885	0.0	22.87087	4
GGACCTG	2330	0.0	22.8506	6
AGGGCCT	135	5.456968E-12	22.815626	5
GATATAC	1170	0.0	22.383648	1
ATACACT	1215	0.0	22.272398	4
>>END_MODULE