FastQCFastQC Report
Thu 2 Feb 2017
SRR4062416_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062416_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505708
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23520.15620558567796677No Hit
GCGCAAGACGGACCAGAGCGAAAGC17590.11682211956103043No Hit
GATTAAGAGGGACGGCCGGGGGCAT16350.10858679106440293No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16310.10832113530644719No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16060.1066607868192239No Hit
GAACTACGACGGTATCTGATCGTCT15890.10553174984791208No Hit
GAATAGGACCGCGGTTCTATTTTGT15410.10234388075244336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1257.2759576E-1213.674932
CGCCAGT2850.013.66932218
GCGTTAT1205.2750693E-1113.4520031
GCATCGC3050.013.08320314
CGCATCG2950.012.88214513
AATTCCG905.4092743E-712.6657575
ATCGCCA3100.012.56653916
AAGACGG2600.012.4221865
GTATCAA18300.012.4012681
CGATAAC1001.4405123E-712.35116810
GTCTCGA550.003066959312.0916511
CGCGTAA1459.640644E-1111.79421810
TCGCGTA1459.640644E-1111.7942189
GCGTAAC1459.640644E-1111.79382511
CATCGCC3400.011.73718415
GCGGGTA658.0100965E-411.69457918
TCGCAGT658.012331E-411.6941916
GTACTAA658.0548885E-411.6868081
AGACCGA907.463206E-611.6106636
GTATAGA1157.118069E-811.5597781
Produced by FastQC (version 0.11.5)