##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062416_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505708
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13939223275695	32.0	32.0	32.0	32.0	32.0
2	30.811624830312386	32.0	32.0	32.0	32.0	32.0
3	30.838787467423963	32.0	32.0	32.0	32.0	32.0
4	30.71769293913561	32.0	32.0	32.0	32.0	32.0
5	30.717511629080803	32.0	32.0	32.0	32.0	32.0
6	34.10791667441496	36.0	36.0	36.0	32.0	36.0
7	34.10249928937085	36.0	36.0	36.0	32.0	36.0
8	34.05253010543877	36.0	36.0	36.0	32.0	36.0
9	34.21785565328736	36.0	36.0	36.0	32.0	36.0
10	33.90195044457491	36.0	36.0	36.0	32.0	36.0
11	34.25706179418586	36.0	36.0	36.0	32.0	36.0
12	34.01800548313484	36.0	36.0	36.0	32.0	36.0
13	34.15699126258212	36.0	36.0	36.0	32.0	36.0
14	34.02514630990869	36.0	36.0	36.0	32.0	36.0
15	33.94257120238453	36.0	36.0	36.0	32.0	36.0
16	33.97130917814078	36.0	36.0	36.0	32.0	36.0
17	33.88818349905825	36.0	36.0	36.0	32.0	36.0
18	33.9075272230738	36.0	36.0	36.0	32.0	36.0
19	33.89061624166173	36.0	36.0	36.0	32.0	36.0
20	33.8383152643142	36.0	36.0	36.0	32.0	36.0
21	33.82807755554198	36.0	36.0	36.0	32.0	36.0
22	33.77549099825464	36.0	36.0	36.0	32.0	36.0
23	33.74891678864694	36.0	36.0	36.0	32.0	36.0
24	33.732487308296164	36.0	36.0	36.0	32.0	36.0
25	33.07232411596405	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	60.0
5	147.0
6	276.0
7	55.0
8	214.0
9	264.0
10	161.0
11	67.0
12	97.0
13	89.0
14	321.0
15	575.0
16	1002.0
17	1264.0
18	1568.0
19	1828.0
20	2373.0
21	3156.0
22	4197.0
23	5709.0
24	7867.0
25	10804.0
26	15302.0
27	20894.0
28	29161.0
29	40876.0
30	57391.0
31	85022.0
32	131381.0
33	199641.0
34	414465.0
35	469476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.717409468346965	17.834147844788077	11.121767580628664	26.32667510623629
2	16.97593036469406	20.011051536422585	36.838211218504945	26.17480688037841
3	19.105929453864867	23.64575370287842	28.38011148651117	28.86820535674555
4	12.700085952767163	15.36628233690562	36.16472641911738	35.76890529120983
5	14.718032874018364	36.25330534036701	33.74948509773177	15.279176687882856
6	34.03562021756984	35.53988470339963	16.84000138207082	13.58449369695971
7	30.14753234393598	30.29234651459074	20.9533942939628	18.60672684751048
8	27.859629344789376	32.83172152401894	19.773905772136317	19.53474335905537
9	27.325232772569596	14.282124475987496	18.860461436801398	39.532181314641505
10	15.94794283366202	26.43889938708558	31.63071939317616	25.982438386076247
11	37.440648315545836	21.13182606706699	22.363442088514383	19.064083528872793
12	24.541500223106965	23.86286934842791	28.654581872276413	22.941048556188708
13	29.129569219680274	19.534590224758997	25.438553329228508	25.897287226332217
14	23.205915814937285	19.94439793576838	24.884949208757757	31.96473704053658
15	24.931950038629243	27.457344941165207	22.16394223033665	25.4467627898689
16	25.68127585929485	25.79862544737453	23.842976278096582	24.677122415234038
17	23.95097060071254	25.803603131562042	25.342007588199856	24.903418679525558
18	24.87335501160386	24.767889560516288	26.39868068439497	23.96007474348488
19	25.496578644624584	25.025435732382846	25.254922562325856	24.223063060666718
20	25.382303462308247	24.620755231363578	25.02363838261598	24.97330292371219
21	26.52384568631076	24.282381762395648	24.387249849380964	24.806522701912627
22	25.64925433689792	24.682971797692574	24.876055375013138	24.79171849039637
23	24.236335982240416	24.400404618395772	25.587325149188068	25.77593425017574
24	24.49774625894946	25.24449333054913	25.2158311177199	25.04192929278151
25	24.520107409757053	24.718541482962202	25.491729475306794	25.269621631973955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	115.0
2	362.0
3	609.0
4	609.0
5	609.0
6	1528.5
7	2448.0
8	2448.0
9	2448.0
10	2828.5
11	3209.0
12	3209.0
13	3209.0
14	4003.5
15	4798.0
16	4798.0
17	4798.0
18	7808.5
19	10819.0
20	10819.0
21	10819.0
22	17480.0
23	24141.0
24	24141.0
25	24141.0
26	35817.5
27	47494.0
28	47494.0
29	47494.0
30	58770.5
31	70047.0
32	70047.0
33	70047.0
34	84879.0
35	99711.0
36	99711.0
37	99711.0
38	111139.5
39	122568.0
40	122568.0
41	122568.0
42	138496.5
43	154425.0
44	154425.0
45	154425.0
46	173081.0
47	191737.0
48	191737.0
49	191737.0
50	192738.0
51	193739.0
52	193739.0
53	193739.0
54	180455.5
55	167172.0
56	167172.0
57	167172.0
58	153330.5
59	139489.0
60	139489.0
61	139489.0
62	125384.0
63	111279.0
64	111279.0
65	111279.0
66	93081.0
67	74883.0
68	74883.0
69	74883.0
70	57347.5
71	39812.0
72	39812.0
73	39812.0
74	31371.5
75	22931.0
76	22931.0
77	22931.0
78	18545.0
79	14159.0
80	14159.0
81	14159.0
82	10017.0
83	5875.0
84	5875.0
85	5875.0
86	4286.5
87	2698.0
88	2698.0
89	2698.0
90	1760.5
91	823.0
92	823.0
93	823.0
94	464.0
95	105.0
96	105.0
97	105.0
98	363.5
99	622.0
100	622.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006774221827871008
2	0.002324487882112601
3	0.00365276667189123
4	0.015275206082454234
5	0.03772311762971307
6	0.04781803643203065
7	0.06780863221819901
8	0.08574039588021051
9	0.09630021225895061
10	0.11595873834767431
11	0.11615798016614111
12	0.12917511230597167
13	0.11861529592723156
14	0.12000998865649914
15	0.1106456231885598
16	0.10991506985418155
17	0.11914660744314301
18	0.1260536571499919
19	0.1273819359397705
20	0.11941226320109875
21	0.1271162801818148
22	0.14704046202849425
23	0.13741044080259918
24	0.13189808382501786
25	0.12844455897159343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1505708.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.45074223460225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33025847297566	43.47162681512598
2	11.54742183277431	12.344365357156759
3	3.1184315334054973	5.00047440204938
4	1.2367147809940089	2.6441329190653335
5	0.6783252673760835	1.8128494508868342
6	0.4046221403432555	1.2976412235540242
7	0.27950309339662466	1.0457753459241859
8	0.19828423201153442	0.8478751499547675
9	0.14292594247520574	0.687554793889183
>10	0.8339881514881644	8.445200100859527
>50	0.10778687149124055	4.060154831522855
>100	0.10706457540395285	11.932985294290267
>500	0.011688745349675095	4.181918796466413
>1k	0.002984360514810663	2.2274455192545797
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2352	0.15620558567796677	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1759	0.11682211956103043	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1635	0.10858679106440293	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1631	0.10832113530644719	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1606	0.1066607868192239	No Hit
GAACTACGACGGTATCTGATCGTCT	1589	0.10553174984791208	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1541	0.10234388075244336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.641393948893145E-5	0.0
10	0.0	0.0	0.0	1.328278789778629E-4	0.0
11	0.0	0.0	0.0	1.328278789778629E-4	0.0
12	0.0	0.0	0.0	1.328278789778629E-4	1.328278789778629E-4
13	0.0	0.0	0.0	1.328278789778629E-4	1.328278789778629E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	125	7.2759576E-12	13.67493	2
CGCCAGT	285	0.0	13.669322	18
GCGTTAT	120	5.2750693E-11	13.452003	1
GCATCGC	305	0.0	13.083203	14
CGCATCG	295	0.0	12.882145	13
AATTCCG	90	5.4092743E-7	12.665757	5
ATCGCCA	310	0.0	12.566539	16
AAGACGG	260	0.0	12.422186	5
GTATCAA	1830	0.0	12.401268	1
CGATAAC	100	1.4405123E-7	12.351168	10
GTCTCGA	55	0.0030669593	12.09165	11
CGCGTAA	145	9.640644E-11	11.794218	10
TCGCGTA	145	9.640644E-11	11.794218	9
GCGTAAC	145	9.640644E-11	11.793825	11
CATCGCC	340	0.0	11.737184	15
GCGGGTA	65	8.0100965E-4	11.694579	18
TCGCAGT	65	8.012331E-4	11.69419	16
GTACTAA	65	8.0548885E-4	11.686808	1
AGACCGA	90	7.463206E-6	11.610663	6
GTATAGA	115	7.118069E-8	11.559778	1
>>END_MODULE