FastQCFastQC Report
Thu 2 Feb 2017
SRR4062416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505708
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17010.11297011107067241No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16570.11004789773315941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT1057.7496225E-820.9523187
GTATCAA20700.019.6637341
GTCGGTT600.007413055718.33327938
GTATTAT2150.017.397031
ACCGTCG3950.017.2657728
TACACGC902.2158767E-417.111065
TACACCG902.2158767E-417.111065
ATACCGT4250.017.08236
GGAGCGT1551.439912E-817.0322076
CCGTCGT4050.016.8394579
CGTCGTA4100.016.63409610
CGGTCCA5950.016.26885810
ACCGTAC953.3470735E-416.2104788
TAATAGA1501.8385253E-716.1332844
CGCAATA5600.016.10709636
ATTTCGT2201.8189894E-1115.99995242
TAATACC1653.112109E-815.9999514
TAGGACG4550.015.95599654
GATATAC3050.015.8703851
TCCTACA7700.015.714762
Produced by FastQC (version 0.11.5)