##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505708
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15447417427549	32.0	32.0	32.0	32.0	32.0
2	31.056902135075326	32.0	32.0	32.0	32.0	32.0
3	31.28191189792443	32.0	32.0	32.0	32.0	32.0
4	31.471532328977464	32.0	32.0	32.0	32.0	32.0
5	31.321698496654065	32.0	32.0	32.0	32.0	32.0
6	34.929775228663196	36.0	36.0	36.0	36.0	36.0
7	34.94952075701265	36.0	36.0	36.0	36.0	36.0
8	34.87082621597282	36.0	36.0	36.0	32.0	36.0
9	35.064210989116084	36.0	36.0	36.0	36.0	36.0
10	34.86605769511751	36.0	36.0	36.0	32.0	36.0
11	35.07710857616483	36.0	36.0	36.0	36.0	36.0
12	34.94692131542105	36.0	36.0	36.0	32.0	36.0
13	35.02432144878024	36.0	36.0	36.0	36.0	36.0
14	34.96902321034357	36.0	36.0	36.0	32.0	36.0
15	34.90523461388264	36.0	36.0	36.0	32.0	36.0
16	34.93183206836917	36.0	36.0	36.0	32.0	36.0
17	34.89553817871725	36.0	36.0	36.0	32.0	36.0
18	34.92118857042667	36.0	36.0	36.0	32.0	36.0
19	34.91064867822978	36.0	36.0	36.0	32.0	36.0
20	34.89810374919971	36.0	36.0	36.0	32.0	36.0
21	34.8957533598812	36.0	36.0	36.0	32.0	36.0
22	34.8776509123947	36.0	36.0	36.0	32.0	36.0
23	34.8315702646197	36.0	36.0	36.0	32.0	36.0
24	34.81038421792273	36.0	36.0	36.0	32.0	36.0
25	34.77550029620617	36.0	36.0	36.0	32.0	36.0
26	34.726848100694156	36.0	36.0	36.0	32.0	36.0
27	34.70634810999211	36.0	36.0	36.0	32.0	36.0
28	34.68884205968222	36.0	36.0	36.0	32.0	36.0
29	34.66688826784476	36.0	36.0	36.0	32.0	36.0
30	34.653140582370554	36.0	36.0	36.0	32.0	36.0
31	34.654169334293236	36.0	36.0	36.0	32.0	36.0
32	34.61823806475093	36.0	36.0	36.0	32.0	36.0
33	34.60467700244669	36.0	36.0	36.0	32.0	36.0
34	34.59106015243327	36.0	36.0	36.0	32.0	36.0
35	34.56375140465482	36.0	36.0	36.0	32.0	36.0
36	34.53065268963172	36.0	36.0	36.0	32.0	36.0
37	34.52968703095155	36.0	36.0	36.0	32.0	36.0
38	34.48889160448108	36.0	36.0	36.0	32.0	36.0
39	34.48731294513943	36.0	36.0	36.0	32.0	36.0
40	34.4792336894006	36.0	36.0	36.0	32.0	36.0
41	34.44740015992477	36.0	36.0	36.0	32.0	36.0
42	34.42612843924586	36.0	36.0	36.0	32.0	36.0
43	34.416133805492166	36.0	36.0	36.0	32.0	36.0
44	34.385032821768895	36.0	36.0	36.0	32.0	36.0
45	34.34163463300985	36.0	36.0	36.0	32.0	36.0
46	34.34397041126168	36.0	36.0	36.0	32.0	36.0
47	34.33713442447009	36.0	36.0	36.0	32.0	36.0
48	34.287934978096686	36.0	36.0	36.0	32.0	36.0
49	34.28300440722902	36.0	36.0	36.0	32.0	36.0
50	33.67128686305711	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	26.0
22	108.0
23	325.0
24	892.0
25	2066.0
26	4417.0
27	8522.0
28	14810.0
29	24209.0
30	37482.0
31	56503.0
32	89094.0
33	154675.0
34	333719.0
35	778849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04594631935256	18.432463453904894	11.786414576989909	26.73517564975264
2	16.522499163155462	20.51026263635253	36.3183887953158	26.648849405176218
3	18.627573391211254	23.861557895407977	28.779742019980105	28.731126693400665
4	12.649157703534225	15.938381024171402	36.31587860835954	35.09658266393483
5	14.804264837538222	36.24102415607807	33.4291243720562	15.525586634327507
6	34.33011867514729	35.81772549373816	16.427764498670726	13.424391332443827
7	30.196890765008888	30.403703772577416	20.798521360051218	18.60088410236248
8	27.963389979996123	32.999957495078725	19.475887755129147	19.560764769796002
9	27.423046168314176	13.984052684849917	18.619878489056312	39.973022657779595
10	15.89876656031581	26.479901813631862	31.567541648181454	26.053789977870878
11	37.80102118073358	21.053816543446672	22.14592736440266	18.999234911417087
12	24.550908941175845	23.83941640743092	28.50121006197749	23.108464589415743
13	29.398661626291418	19.34206366705895	25.21870110273705	26.040573603912577
14	23.304518538787068	19.81572788349401	24.841669168258388	32.03808440946053
15	25.16224925417146	27.440380206520786	21.78676077964652	25.610609759661234
16	25.932717366182555	25.798162724777978	23.607166861038127	24.66195304800134
17	24.21864000191272	25.82346643572326	24.995085368477817	24.9628081938862
18	25.21325515969896	24.793386234249933	25.933115849819487	24.06024275623162
19	25.735467965900426	24.95397513993417	25.031945104894177	24.278611789271228
20	25.674035071873163	24.593613104267227	24.733613688709895	24.998738135149708
21	26.83050100019393	24.36661025909406	24.056789231378197	24.746099509333817
22	25.768077210189492	24.724448565060424	24.58856564486607	24.918908579884015
23	24.45859356528623	24.428043153121322	25.35445119505243	25.758912086540022
24	24.833234597943292	25.174137349339976	24.871090543451984	25.121537509264748
25	24.899117225916314	24.744837644483525	25.1188145377457	25.237230591854466
26	24.554628121787225	25.452677411556557	25.42876839334054	24.563926073315674
27	25.455865280652024	24.988842458165863	24.59035882123227	24.964933439949842
28	24.398090466411816	24.918244440489126	25.569433117178097	25.114231975920966
29	24.545462998137754	25.03978194975387	25.175199972371804	25.239555079736576
30	24.451686515579382	25.097827732867195	25.609347894810945	24.841137856742478
31	25.095636072864064	24.91678333382037	24.58863205880556	25.398948534510012
32	24.84406007007999	25.023842604276524	24.648404604345597	25.483692721297892
33	24.507009327173662	24.67868936075255	25.201831962106862	25.612469349966926
34	24.98711569573915	24.859202448283465	25.578000515372167	24.575681340605218
35	25.757982291387176	24.63200036129183	25.15461165113023	24.455405696190763
36	24.578404312124263	25.07896617405234	25.02470598548988	25.317923528333512
37	25.54970817714989	25.014743894566543	24.541610989647396	24.893936938636177
38	24.578005828487328	24.838813368860365	25.31114930650564	25.272031496146663
39	25.165437123266926	24.63140263583643	24.70279762078703	25.500362620109613
40	25.48927149221496	24.693765325016535	25.316595249543738	24.50036793322477
41	24.337255297839953	24.983263687248787	25.78295393263501	24.896527082276247
42	25.62289633846669	25.242610120953067	25.19877692089037	23.935716619689874
43	24.669258581345122	24.174740387910536	25.540675881379393	25.61532514936495
44	24.286913531707345	24.820815191258863	25.316196765906803	25.57607451112699
45	24.465633442872058	25.07684092798869	25.342031788367997	25.11549384077125
46	24.316351056347614	24.63500534964638	25.50496212078446	25.543681473221554
47	24.60404009276699	24.42571866523921	25.880781665502212	25.089459576491592
48	25.146841220210025	25.22766698456806	24.937438069001427	24.688053726220488
49	24.18901938490066	25.133956915949174	25.38586498843069	25.291158710719476
50	24.079967696259832	25.39914777632848	25.11130976258345	25.409574764828243
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.5
2	15.0
3	44.0
4	73.0
5	91.0
6	109.0
7	122.0
8	135.0
9	170.0
10	205.0
11	285.0
12	365.0
13	674.0
14	983.0
15	1347.5
16	1712.0
17	2193.0
18	2674.0
19	3147.0
20	3620.0
21	4713.0
22	5806.0
23	6516.0
24	7226.0
25	9292.5
26	11359.0
27	14672.5
28	17986.0
29	20517.0
30	23048.0
31	24904.0
32	26760.0
33	30607.5
34	34455.0
35	40430.0
36	46405.0
37	54085.5
38	61766.0
39	65289.5
40	68813.0
41	74342.5
42	79872.0
43	80475.0
44	81078.0
45	87584.0
46	94090.0
47	99796.5
48	105503.0
49	108530.0
50	111557.0
51	106928.5
52	102300.0
53	100058.0
54	97816.0
55	99552.0
56	101288.0
57	99905.5
58	98523.0
59	89658.0
60	80793.0
61	71483.5
62	62174.0
63	55266.5
64	48359.0
65	42090.0
66	35821.0
67	31914.5
68	28008.0
69	25831.5
70	23655.0
71	18517.0
72	13379.0
73	12047.0
74	10715.0
75	8073.5
76	5432.0
77	4840.5
78	4249.0
79	3688.0
80	3127.0
81	2477.5
82	1828.0
83	1556.0
84	1284.0
85	1023.5
86	763.0
87	574.0
88	385.0
89	250.0
90	115.0
91	77.5
92	40.0
93	26.5
94	13.0
95	9.5
96	6.0
97	7.0
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014079755171653467
2	0.003453524853424435
3	6.641393948893145E-4
4	1.9924181846679435E-4
5	0.0
6	6.641393948893145E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.641393948893145E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1505708.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.63407852395114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.37445158388402	47.47575610952983
2	11.229890483436458	12.94448779875497
3	2.874308833521912	4.969744230398649
4	1.1122281353275263	2.564089747520576
5	0.5963257575244636	1.7184342767509793
6	0.35874353921478075	1.2405511985438893
7	0.25360107684665906	1.0231245063717243
8	0.1745809016472451	0.8049447515455613
9	0.12910745010684804	0.6696890025736665
>10	0.7079670444903066	7.691630258983101
>50	0.092620837653228	3.7312424069186036
>100	0.08610565468624073	10.463738502838675
>500	0.007754057600503011	3.000086067608447
>1k	0.002314644059851645	1.702481141661212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1701	0.11297011107067241	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1657	0.11004789773315941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.328278789778629E-4
7	0.0	0.0	0.0	0.0	1.328278789778629E-4
8	0.0	0.0	0.0	0.0	1.328278789778629E-4
9	0.0	0.0	0.0	0.0	1.328278789778629E-4
10	0.0	0.0	0.0	0.0	1.328278789778629E-4
11	0.0	0.0	0.0	0.0	1.328278789778629E-4
12	0.0	0.0	0.0	0.0	1.328278789778629E-4
13	0.0	0.0	0.0	0.0	1.9924181846679435E-4
14	0.0	0.0	0.0	0.0	1.9924181846679435E-4
15	0.0	0.0	0.0	0.0	3.984836369335887E-4
16	0.0	0.0	0.0	0.0	4.6489757642252015E-4
17	0.0	0.0	0.0	6.641393948893145E-5	4.6489757642252015E-4
18	0.0	0.0	0.0	1.328278789778629E-4	5.313115159114516E-4
19	0.0	0.0	0.0	1.9924181846679435E-4	5.977254554003831E-4
20	0.0	0.0	0.0	4.6489757642252015E-4	6.641393948893145E-4
21	0.0	0.0	0.0	5.313115159114516E-4	6.641393948893145E-4
22	0.0	0.0	0.0	7.30553334378246E-4	7.30553334378246E-4
23	0.0	0.0	0.0	0.001328278789778629	7.30553334378246E-4
24	6.641393948893145E-5	0.0	0.0	0.0016603484872232863	7.30553334378246E-4
25	6.641393948893145E-5	0.0	0.0	0.002191660003134738	7.30553334378246E-4
26	6.641393948893145E-5	0.0	0.0	0.002922213337512984	7.30553334378246E-4
27	6.641393948893145E-5	0.0	0.0	0.00411766424831375	7.969672738671774E-4
28	6.641393948893145E-5	0.0	0.0	0.00916512364947254	7.969672738671774E-4
29	6.641393948893145E-5	0.0	0.0	0.01872873093587867	7.969672738671774E-4
30	6.641393948893145E-5	0.0	0.0	0.04230567945444934	7.969672738671774E-4
31	6.641393948893145E-5	0.0	0.0	0.09158482255523648	7.969672738671774E-4
32	6.641393948893145E-5	0.0	0.0	0.14704046202849425	7.969672738671774E-4
33	6.641393948893145E-5	0.0	0.0	0.2056839705972207	7.969672738671774E-4
34	6.641393948893145E-5	0.0	0.0	0.2743559840287758	9.297951528450403E-4
35	6.641393948893145E-5	0.0	0.0	0.3490052520143348	9.297951528450403E-4
36	6.641393948893145E-5	0.0	0.0	0.45679507580487055	9.297951528450403E-4
37	6.641393948893145E-5	0.0	0.0	0.6189779160368412	9.297951528450403E-4
38	6.641393948893145E-5	0.0	0.0	0.8384095721082706	9.962090923339717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGTT	105	7.7496225E-8	20.952318	7
GTATCAA	2070	0.0	19.663734	1
GTCGGTT	60	0.0074130557	18.333279	38
GTATTAT	215	0.0	17.39703	1
ACCGTCG	395	0.0	17.265772	8
TACACGC	90	2.2158767E-4	17.11106	5
TACACCG	90	2.2158767E-4	17.11106	5
ATACCGT	425	0.0	17.0823	6
GGAGCGT	155	1.439912E-8	17.032207	6
CCGTCGT	405	0.0	16.839457	9
CGTCGTA	410	0.0	16.634096	10
CGGTCCA	595	0.0	16.268858	10
ACCGTAC	95	3.3470735E-4	16.210478	8
TAATAGA	150	1.8385253E-7	16.133284	4
CGCAATA	560	0.0	16.107096	36
ATTTCGT	220	1.8189894E-11	15.999952	42
TAATACC	165	3.112109E-8	15.999951	4
TAGGACG	455	0.0	15.9559965	4
GATATAC	305	0.0	15.870385	1
TCCTACA	770	0.0	15.71476	2
>>END_MODULE