FastQCFastQC Report
Thu 2 Feb 2017
SRR4062415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787428
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37220.13352811265438966No Hit
GCGCAAGACGGACCAGAGCGAAAGC29060.10425381391017095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.012.47260951
CGAGCTA853.942312E-612.295279514
ACGGTAT4650.012.0549049
CAAGACG5900.011.9129574
TCGCGTA1701.8189894E-1211.73640259
GTATCAA27000.011.7093611
CGTCGTA4000.011.6388110
CGAGCCG5250.011.58225415
GCGTTAT2800.011.5285211
CGCAAGA5450.011.499272
CGGTCCA4950.011.32450610
ACGGTAC600.00586800711.08577517
CGACCAT3600.011.0845810
AGAACCG3100.011.0317155
AACCGCG2250.010.9782247
AAGACGG6000.010.9244635
ACGCTTA700.001489305410.85953517
CGCATCG2650.010.755733513
CCGTCGT4350.010.7021619
GCGTAAC2050.010.65935211
Produced by FastQC (version 0.11.5)