##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062415_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2787428
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23563514465665	32.0	32.0	32.0	32.0	32.0
2	30.90025284958033	32.0	32.0	32.0	32.0	32.0
3	30.877184989172815	32.0	32.0	32.0	32.0	32.0
4	30.955315796497704	32.0	32.0	32.0	32.0	32.0
5	30.76184353461327	32.0	32.0	32.0	32.0	32.0
6	34.415370011350966	36.0	36.0	36.0	32.0	36.0
7	34.321702300471976	36.0	36.0	36.0	32.0	36.0
8	34.33289613220503	36.0	36.0	36.0	32.0	36.0
9	34.47583399463591	36.0	36.0	36.0	32.0	36.0
10	34.1660638409315	36.0	36.0	36.0	32.0	36.0
11	34.43659997675276	36.0	36.0	36.0	32.0	36.0
12	34.234991181835014	36.0	36.0	36.0	32.0	36.0
13	34.34854783693068	36.0	36.0	36.0	32.0	36.0
14	34.26491267218382	36.0	36.0	36.0	32.0	36.0
15	34.20115568904381	36.0	36.0	36.0	32.0	36.0
16	34.20181436076555	36.0	36.0	36.0	32.0	36.0
17	34.14087000632841	36.0	36.0	36.0	32.0	36.0
18	34.14225407795286	36.0	36.0	36.0	32.0	36.0
19	34.140588025950805	36.0	36.0	36.0	32.0	36.0
20	34.141976043865526	36.0	36.0	36.0	32.0	36.0
21	34.125692215189055	36.0	36.0	36.0	32.0	36.0
22	34.082411456008906	36.0	36.0	36.0	32.0	36.0
23	34.049290241756914	36.0	36.0	36.0	32.0	36.0
24	34.01655791647354	36.0	36.0	36.0	32.0	36.0
25	33.57945604334892	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	108.0
5	232.0
6	485.0
7	121.0
8	415.0
9	485.0
10	316.0
11	102.0
12	208.0
13	158.0
14	394.0
15	463.0
16	811.0
17	1101.0
18	1467.0
19	1909.0
20	3021.0
21	4376.0
22	6566.0
23	9983.0
24	14744.0
25	21421.0
26	30272.0
27	40117.0
28	54784.0
29	74013.0
30	96387.0
31	132000.0
32	190331.0
33	275117.0
34	623549.0
35	1201961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28965931445272	18.013743379186455	11.402993578218972	26.29360372814185
2	16.99554629464199	19.96378628815553	36.71314822508325	26.32751919211923
3	18.734086205096503	23.42539311580489	28.927920128670102	28.9126005504285
4	12.915481865136247	15.422375391769389	35.990398398297835	35.67174434479653
5	14.669216615628212	36.17567229543137	33.94434732802648	15.210763760913935
6	34.013692120390736	35.37001889360432	16.796931081414783	13.819357904590168
7	29.96619398556648	30.70890677373161	21.052599458859657	18.272299781842253
8	27.632727951684366	32.95645991770257	19.834543378503568	19.5762687521095
9	26.918236102916858	14.686095393906445	19.194803525266167	39.200864977910534
10	15.93223869821869	26.714568177042835	31.923704782548572	25.429488342189906
11	36.73158668843238	21.453849317307156	22.63885970630649	19.17570428795397
12	24.10527941720579	24.060664623917408	28.934956516741323	22.899099442135476
13	28.707570465218602	19.68651395835391	25.49476954573617	26.11114603069132
14	22.97543631282127	20.028634347783765	25.340068798582223	31.655860540812746
15	24.711459310519203	27.454247948179038	22.399901449383627	25.43439129191813
16	25.5298486557151	26.16384976188595	23.75534230241562	24.550959279983335
17	23.872044608169926	26.177738730144135	25.410514988161193	24.539701673524746
18	24.44754720261446	25.306557017252395	26.21883736610076	24.027058414032386
19	25.081281114654956	25.535722560461977	25.60303923656998	23.779957088313086
20	25.05525039482527	25.042320024510232	25.19655060821229	24.705878972452204
21	25.94370934671692	24.6821015637011	24.967850949097457	24.406338140484525
22	25.230666200561842	24.829638805885423	25.116595068318727	24.823099925234008
23	24.215311396683205	24.597291615474923	25.736550402133602	25.45084658570827
24	24.376811406779638	25.375141715413896	25.350283143445456	24.89776373436101
25	24.42476846156029	24.994548972854062	25.57233988977251	25.00834267581314
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	270.0
1	270.0
2	692.0
3	1114.0
4	1114.0
5	1114.0
6	2847.0
7	4580.0
8	4580.0
9	4580.0
10	5464.5
11	6349.0
12	6349.0
13	6349.0
14	8425.0
15	10501.0
16	10501.0
17	10501.0
18	16699.5
19	22898.0
20	22898.0
21	22898.0
22	35233.5
23	47569.0
24	47569.0
25	47569.0
26	68304.0
27	89039.0
28	89039.0
29	89039.0
30	108212.0
31	127385.0
32	127385.0
33	127385.0
34	156040.0
35	184695.0
36	184695.0
37	184695.0
38	208470.0
39	232245.0
40	232245.0
41	232245.0
42	261244.5
43	290244.0
44	290244.0
45	290244.0
46	321503.0
47	352762.0
48	352762.0
49	352762.0
50	358971.5
51	365181.0
52	365181.0
53	365181.0
54	343895.0
55	322609.0
56	322609.0
57	322609.0
58	294014.0
59	265419.0
60	265419.0
61	265419.0
62	232295.0
63	199171.0
64	199171.0
65	199171.0
66	162864.5
67	126558.0
68	126558.0
69	126558.0
70	96082.0
71	65606.0
72	65606.0
73	65606.0
74	50679.5
75	35753.0
76	35753.0
77	35753.0
78	28884.5
79	22016.0
80	22016.0
81	22016.0
82	15297.5
83	8579.0
84	8579.0
85	8579.0
86	6342.0
87	4105.0
88	4105.0
89	4105.0
90	2751.5
91	1398.0
92	1398.0
93	1398.0
94	839.5
95	281.0
96	281.0
97	281.0
98	691.0
99	1101.0
100	1101.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0069956963910816706
2	0.003085281485297558
3	0.005201928085676114
4	0.014099018880487675
5	0.034691479026543466
6	0.046637975940544474
7	0.06561604461173526
8	0.0863878815883316
9	0.09388583310492683
10	0.11634381228860441
11	0.1159850586275233
12	0.12907956725698386
13	0.11670256594968553
14	0.1205053547571453
15	0.1102091246841174
16	0.10934811589752275
17	0.12068473158768586
18	0.12646066553109175
19	0.12811093237206486
20	0.11785057766514506
21	0.1262812887005512
22	0.14626386762276908
23	0.13546538242422765
24	0.13202134727784898
25	0.12732167431768643
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2787428.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.72094691952045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17471675036238	44.96678313423208
2	10.568012821643919	11.56583337315977
3	2.8820997782487017	4.731336869469266
4	1.3001946633106465	2.8459153262426264
5	0.7325502512315505	2.0042921706761523
6	0.4591109242287905	1.5073790708937373
7	0.31605960265016597	1.2106556518017122
8	0.23935306473462392	1.0478101080294318
9	0.17293237891186886	0.8516721174392496
>10	0.9810434665567835	9.659143310575198
>50	0.08133184894971607	3.112327617117354
>100	0.07731172580463348	9.014658978807677
>500	0.011593790139988298	4.374167556704716
>1k	0.0036889332263449464	3.1080247148511853
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3722	0.13352811265438966	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2906	0.10425381391017095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.587536610811113E-5
2	0.0	0.0	0.0	0.0	3.587536610811113E-5
3	0.0	0.0	0.0	3.587536610811113E-5	3.587536610811113E-5
4	0.0	0.0	0.0	3.587536610811113E-5	3.587536610811113E-5
5	0.0	0.0	0.0	7.175073221622226E-5	3.587536610811113E-5
6	3.587536610811113E-5	0.0	0.0	7.175073221622226E-5	3.587536610811113E-5
7	3.587536610811113E-5	0.0	0.0	1.4350146443244452E-4	3.587536610811113E-5
8	3.587536610811113E-5	0.0	0.0	1.7937683054055565E-4	3.587536610811113E-5
9	3.587536610811113E-5	0.0	0.0	2.152521966486668E-4	3.587536610811113E-5
10	7.175073221622226E-5	0.0	0.0	2.152521966486668E-4	3.587536610811113E-5
11	7.175073221622226E-5	0.0	0.0	2.152521966486668E-4	3.587536610811113E-5
12	7.175073221622226E-5	0.0	0.0	2.152521966486668E-4	3.587536610811113E-5
13	7.175073221622226E-5	0.0	0.0	2.5112756275677794E-4	7.175073221622226E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1020	0.0	12.4726095	1
CGAGCTA	85	3.942312E-6	12.2952795	14
ACGGTAT	465	0.0	12.054904	9
CAAGACG	590	0.0	11.912957	4
TCGCGTA	170	1.8189894E-12	11.7364025	9
GTATCAA	2700	0.0	11.709361	1
CGTCGTA	400	0.0	11.63881	10
CGAGCCG	525	0.0	11.582254	15
GCGTTAT	280	0.0	11.528521	1
CGCAAGA	545	0.0	11.49927	2
CGGTCCA	495	0.0	11.324506	10
ACGGTAC	60	0.005868007	11.085775	17
CGACCAT	360	0.0	11.08458	10
AGAACCG	310	0.0	11.031715	5
AACCGCG	225	0.0	10.978224	7
AAGACGG	600	0.0	10.924463	5
ACGCTTA	70	0.0014893054	10.859535	17
CGCATCG	265	0.0	10.7557335	13
CCGTCGT	435	0.0	10.702161	9
GCGTAAC	205	0.0	10.659352	11
>>END_MODULE