FastQCFastQC Report
Thu 2 Feb 2017
SRR4062415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787428
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30450.023.125931
GGTATCA14450.019.9498651
TACCGTC5250.019.2760567
ATACCGT5700.018.1402246
TAACGGC4500.017.5998836
GTATTAG9200.017.4610981
ACCGTCG5850.017.2990258
TAGATCG1551.4415491E-817.032145
GTGTACG1451.2472083E-716.694031
AACGCAG42700.016.6415716
CGTCGTA6100.016.59004810
TCAACGC42650.016.5063344
CGCAATA8050.016.39740236
TTAACGG4900.016.16315535
TATCAAC44750.016.0272792
TAGGACG2201.8189894E-1115.9998894
CAACGCA44200.015.9772645
TATAACG850.002980671615.5298612
TAGCGGC9050.015.3148130
CTAGCGG8950.015.24011729
Produced by FastQC (version 0.11.5)