FastQCFastQC Report
Thu 2 Feb 2017
SRR4062414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171921
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG66220.5650551530350595No Hit
TCCATGTACTCTGCGTTGATACCAC61050.5209395513861429No Hit
GTATCAACGCAGAGTACTTTTTTTT40820.348316994063593No Hit
GAGTACATGGAAGCAGTGGTATCAA30520.26042711070114793No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29750.2538567019449263No Hit
CATGTACTCTGCGTTGATACCACTG28510.2432757839478941No Hit
TATCAACGCAGAGTACTTTTTTTTT27310.23303618588625002No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21830.18627535473807535No Hit
GCGCAAGACGGACCAGAGCGAAAGC18760.1600790496970359No Hit
CCCATGTACTCTGCGTTGATACCAC18700.1595670697939537No Hit
GCGTTGATACCACTGCTTCCATGTA18250.15572722052083715No Hit
GCTTCCATGTACTCTGCGTTGATAC18050.15402062084389648No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16720.14267173299224092No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16260.1387465537352774No Hit
GTACATGGGAAGCAGTGGTATCAAC15670.13371208468830237No Hit
GTATCAACGCAGAGTACATGGAAGC15480.13209081499520872No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15360.13106685518904432No Hit
CATGGAAGCAGTGGTATCAACGCAG15130.12910426556056254No Hit
GAATAGGACCGCGGTTCTATTTTGT14720.12560573622283414No Hit
GTATCTGATCGTCTTCGAACCTCCG13850.11818202762814217No Hit
GAACTACGACGGTATCTGATCGTCT13690.11681674788658963No Hit
GGTATCAACGCAGAGTACTTTTTTT13550.11562212811273116No Hit
GATTAAGAGGGACGGCCGGGGGCAT12840.10956369925959172No Hit
GAATAACGCCGCCGCATCGCCAGTC12750.10879572940496841No Hit
GTCCTATTCCATTATTCCTAGCTGC12540.10700379974418071No Hit
TCGTAGTTCCGACCATAAACGATGC12260.10461456019646374No Hit
GGGTAGGCACACGCTGAGCCAGTCA12210.10418791027722858No Hit
ACGCAGAGTACATGGAAGCAGTGGT12080.10307862048721714No Hit
ACTCTGCGTTGATACCACTGCTTCC12070.10299329050337011No Hit
CTATTGGAGCTGGAATTACCGCGGC11930.10179867072951164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT250.00602909419.00115212
CGCATCG1900.013.49966713
GCTCGTA852.6936868E-713.4131539
CGGCTAA655.4449592E-513.1552079
CGGTCCA3100.012.87229910
GTCCTAG751.483423E-512.6620261
GGTCCAA3550.012.04298411
ATTACAC951.0436415E-611.99560453
TCGCGTA1353.6925485E-1011.9642019
AATCGCT802.8656703E-511.87470515
GATCTAC802.8757093E-511.8706491
CGCCAGT2100.011.76664118
AAGCTCG1052.7153146E-711.7626197
GCATCGC2200.011.65880214
AGAATTT3350.011.63005617
CGGACAT1800.011.608845
CAAGACG2950.011.5904584
GTCCTAT2950.011.5889731
TTTGCGC2050.011.58854517
GTCTTGA1157.10188E-811.5609811
Produced by FastQC (version 0.11.5)